Vector Name:
pJFT7_nHALO_DC
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable HALO tag; ampicillin resistance; Gateway or Flexi cloning compatible
Comments:
Modified from pANT7_cGST, can be used for either Gateway or Flexi cloning, contains Gateway Death Cassette
Size (bp):
6616
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Justin Saul
Fernanda Festa
Justin Saul
Fernanda Festa
Publications:
PMID:
201200062
Title: A versatile protein microarray platform enabling antibody profiling against denatured proteins.
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3451
4133
bacterial origin
M13 Ori
M13 origin
5527
5982
death cassette
ccdB
ccdB death cassette (removed in plasmids with an insert)
1483
3137
death cassette 2
ccdB (BsrGI removed)
ccdB death cassette with BsrGI restriction site removed
2692
2997
enhancer
CITE
cap-independent translational enhancer (CITE)
17
515
primer
HaloseqF
HaloseqF forward sequencing primer aagcctgcctaactgcaa
1289
1306
primer
NAP150
NAP150 forward sequencing primer cccattgtatgggatctgatc
388
408
primer
pANT1491R
pANT1491R reverse sequencing primer
3155
3176
primer
M13 forward
M13 forward sequencing primer
6126
6143
promoter
T7
T7 promoter
6142
6166
protease cleavage site
TEV
TEV protease cleavage site (EDLYFQS)
1422
1442
recombination site
attR1
attR1 recombinational cloning site
1458
1482
recombination site
attR2
attR2 recombinational cloning site
3138
3162
reporter gene
LacZ
LacZ alpha gene
5987
6055
selectable marker
AmpR
ampicillin resistance gene
4231
4890
selectable marker
CmR
chloramphenicol resistance gene (removed in plasmids with an insert)
1691
2347
tag
HALO
N-terminal HALO tag
519
1409
trxn termination sequence
T7 term
T7 termination
3211
3229
