Detailed Vector Information: pSGP18

Vector Name:
pSGP18
Synonyms:
''
Sequencing Primer:
Forward:  pSGP18for
Reverse:  Unknown
Description:
Yeast expression vector with a AOX1 promoter C-terminal CBP and RSG-6xHis tag with 3C protease cleavage site; zeocin resistance in Pichia and in bacteria; ligation independent cloning (LIC)
Comments:
Similar to pSGP17 but without the prepro-alpha-factor signal sequence
Size (bp):
3424
Parent Vector:
None
Properties:
expression in Pichia pastoris, ligation independent cloning (LIC), with tag/fusion/marker, yeast expression
Author Name:
University of Rochester
Mark Dumont
Sara M Connelly
Membrane Protein Structural Biology Consortium
PSI:Biology
Publications:
PMID: 16893193
Title: Purification and ATP hydrolysis of the putative cholesterol transporters ABCG5 and ABCG8
PMID: 17569508
Title: Expression of 25 human ABC transporters in the yeast Pichia pastoris and characterization of the purified ABCC3 ATPase activity
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
bacterial origin
pUC ori
pUC origin for bacterial replication
2738
3420
ligation independent cloning
LIC
LIC cloning site (using an inverted pair of BsmBI sites with a PstI site in between)
956
975
primer
primer
forward sequencing primer CACCTGTGCCGAAACGCAAATG
625
646
promoter
AOX1
Pichia AOX1 promoter
7
935
protease cleavage site
3C
3C protease cleavage site
988
1011
selectable marker
ZeoR
Zeocin resistance gene
1994
2368
tag
His
C-terminal RGS-6xHis tag
1151
1168
tag
CBP
C-terminal calmodulin binding peptide (CBP) tag (start and end location estimated)
1012
1150