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Detailed Vector Information: pJFT7_cHalo_empty


Vector Name: pJFT7_cHalo_empty
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Synonyms: None


Sequencing Primer: Forward:  NAP150
Reverse:  cHaloseqR
Description: In vitro expression vector with T7 promoter and C-terminal TEV-cleavable Halo tag; does not contain a death cassette; ampicillin resistance; restriction enzyme cloning
Comments: Modified from pANT7_cGST, expresses Halo without an insert
Size (bp): 4520
Empty Vector: EvNO00424544
Parent Vector: None
Properties: cell-free expression, multiple cloning site, with tag/fusion/marker
Author Name: Joshua LaBaer
Center for Personalized Diagnostics
Fernanda Festa
Justin Saul
Publications: None
Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
bacterial origin M13 Ori M13 origin 3881 4336
bacterial origin ColE ColE1 origin 1805 2487
enhancer CITE cap-independent translational enhancer (CITE) 17 515
primer cHaloseqR cHalo reverse sequencing primer CAACATCGACGTAGTGCAT 666 684
primer M13 forward M13 forward sequencing primer 4480 4497
primer HaloseqF HaloseqF forward sequencing primer aagcctgcctaactgcaa 1373 1390
promoter T7 T7 promoter 4496 4520
protease cleavage site TEV TEV protease cleavage site (EDLYFQS) 573 593
reporter gene LacZ LacZ alpha gene 4341 4409
selectable marker AmpR ampicillin resistance gene 2585 3244
tag HALO C-terminal Halo tag 609 1493
trxn termination sequence T7 term T7 terminator 1565 1583