Vector Name:
pMCSG48
Synonyms:
''
Sequencing Primer:
Forward:
pMCSG48F
Reverse:
T7 terminator
Description:
Bacterial expression vector with a T7 promoter and N-terminal 8xHis NusA tag, TEV cleavable; ampicillin resistance in bacteria; Ligation Independent cloning
Comments:
None
Size (bp):
6741
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Not Available
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR322 origin of replication
4735
4735
gene fragment
NusA
NusA cds
252
1710
ligation independent cloning
LIC
LIC site
205
234
primer
forward primer
Forward sequencing primer GAAGGGTTGACCGACGAAAAAGC
0
0
promoter
T7
T7 promoter
2345
3304
protease cleavage site
TEV
TEV protease cleavage site
223
243
repressor protein gene
LacI
LacI cds
2345
3304
selectable marker
AmpR
Ampicillin resistance gene
5493
6353
tag
His
N-terminal 8xHis tag
1717
1740
trxn termination sequence
T7 term
T7 terminator
26
72
