Detailed Vector Information: pET16b

Vector Name:
pET16b
Synonyms:
''
Sequencing Primer:
Forward:  T7
Reverse:  T7 terminator
Description:
Bacterial expression vector with a T7 promoter and Factor Xa cleavable N-terminal 6xHis tag; ampicillin resistance in bacteria; restriction enzyme cloning.
Comments:
None
Size (bp):
5711
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name:
Novagen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
DNA Vector Map
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Vector Features:

Type
Name
Description
Start
End
MCS
MCS
Multiple cloning sites (NdeI - BamHI)
319
335
bacterial origin
pBR322 origin
pBR322 origin
3885
3885
promoter
T7
T7 promoter
466
482
protease cleavage site
Factor X
Factor Xa protease cleavage site
402
412
repressor protein gene
LacI
LacI coding sequence
869
1948
selectable marker
AmpR
ampicillin resistance
4646
5503
tag
His
N-terminal 6xHis tag
360
389
trxn regulatory element
lac operator
lac operator
441
463
trxn termination sequence
T7 term
T7 terminator
213
259