Vector Name:
pMCSG53
Synonyms:
''
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector with a T7 promoter and TEV-cleavable N-terminal 6xHis tag; Contains rare argU and ileX tRNA genes; ampicillin resistance in bacteria; ligation independent cloning.
Comments:
1 kb of sequence (including the rop repressor) removed from pMCSG52 by digestion with PshAI and Tth111I, followed by treatment with Klenow, dNTPs and T4 DNA ligase
Size (bp):
4808
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
PSI:Biology
Argonne National Laboratory
PSI:Biology
Argonne National Laboratory
Publications:
PMID:
24057978
Title: New LIC vectors for production of proteins from genes containing rare codons
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR322 origin
2801
3389
ligation independent cloning
LIC
Ligation independent cloning site
204
235
promoter
T7
T7 promoter
360
376
protease cleavage site
TEV
TEV protease cleavage site
223
243
repressor protein gene
LacI
LacI CDS
1413
2372
selectable marker
AmpR
ampicillin resistance gene
3560
4420
tRNA
ileX
ileX tRNA gene
959
1110
tRNA
argU
argU tRNA gene
443
799
tag
His
N-terminal 6xHis tag
268
285
trxn termination sequence
T7 term
T7 terminator
26
72
