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Detailed Vector Information: pMCSG53


Vector Name: pMCSG53
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Synonyms: None


Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with a T7 promoter and TEV-cleavable N-terminal 6xHis tag; Contains rare argU and ileX tRNA genes; ampicillin resistance in bacteria; ligation independent cloning.
Comments: 1 kb of sequence (including the rop repressor) removed from pMCSG52 by digestion with PshAI and Tth111I, followed by treatment with Klenow, dNTPs and T4 DNA ligase
Size (bp): 4808
Empty Vector: EvNO00450863
Parent Vector: None
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Argonne National Laboratory
Publications: PMID: 24057978
Title: New LIC vectors for production of proteins from genes containing rare codons

Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 origin 2801 3389
ligation independent cloning LIC Ligation independent cloning site 204 235
promoter T7 T7 promoter 360 376
protease cleavage site TEV TEV protease cleavage site 223 243
repressor protein gene LacI LacI CDS 1413 2372
selectable marker AmpR ampicillin resistance gene 3560 4420
tRNA argU argU tRNA gene 443 799
tRNA ileX ileX tRNA gene 959 1110
tag His N-terminal 6xHis tag 268 285
trxn termination sequence T7 term T7 terminator 26 72