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Detailed Vector Information: pMCSG68

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Vector Name: pMCSG68
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Synonyms: None

pMCSG68

Sequencing Primer: Forward:  T7short
Reverse:  T7 terminator
Description: Bacterial expression vector with a T7 promoter and TEV-cleavable N-terminal 6xHis and StrepII tags; rare argU and ileX codons; ampicillin resistance in bacteria; ligation independent cloning
Comments: BglI-XbaI fragment removed from pMCSG57 and ligated into pMCSG53 digested with the same enzymes
Size (bp): 4832
Empty Vector: EvNO00450864
Parent Vector: None
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI:Biology
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 origin of replication 2825 3413
ligation independent cloning LIC ligation independent cloning site 204 235
promoter T7 T7 promoter 384 400
protease cleavage site TEV TEV protease cleavage site 223 243
repressor protein gene LacI Lac I gene 1437 2396
selectable marker AmpR ampicillin resistance gene 3584 4444
tRNA ileX ileX tRNA gene 983 1134
tRNA argU argU tRNA gene 467 853
tag His N-terminal 6xHis tag 292 309
tag strep N-terminal strepII tag 250 273
trxn regulatory element lac operator lac operator sequence 359 381
trxn termination sequence T7 term T7 terminator 26 72