Vector Name:
pMCSG69
Synonyms:
''
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector with a T7 promoter and N-terminal MBP tag, TVMV cleavage site, 6xHis tag and TEV protease cleavage site with rare codon argU and ileX tRNA genes; ampicillin resistance in bacteria; ligation independent cloning
Comments:
LIC region plus a portion of the beta-lactamase gene from pMCSG19 removed by digestion with BglI and XbaI then ligated into pMCSG53 digested with the same enzymes. Product is pMCSG19 with truncated ori and the argU & ileX tRNA genes
Size (bp):
58963
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
Publications:
PMID:
24057978
Title: New LIC vectors for production of proteins from genes containing rare codons
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR332 origin of replication
3956
4544
ligation independent cloning
LIC
ligation independent cloning site
204
235
promoter
T7
T7 promoter
1515
1531
protease cleavage site
TEV
TEV protease cleavage site
223
243
protease cleavage site
TVMV
TVMV cleavage site
286
306
repressor protein gene
LacI
LacI gene
2568
3527
selectable marker
AmpR
ampicillin resistance gene
4715
5575
tRNA
ileX
ileX tRNA gene
2114
2265
tRNA
argU
argU tRNA gene
1598
1954
tag
His
N-terminal 6xHis tag
268
285
tag
MBP
N-terminal maltose binding protein (MBP) tag
337
1443
trxn termination sequence
T7 term
T7 termination sequence
26
72
