Vector Name:
pMCSG54
Synonyms:
''
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector with a T7 promoter and N-terminal Flag and 6xHiis tag followed by TEV protease cleavage site ; ampicillin resistance in bacteria; ligation independent cloning.
Comments:
origin of replication similar to pUC vectors
Size (bp):
4302
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR322 origin of replication
2295
2883
ligation independent cloning
LIC
ligation independent cloning site
204
235
protease cleavage site
TEV
TEV protease cleavage site
223
243
repressor binding site
lac operator
LacO fragment
359
381
repressor protein gene
LacI
Lac I
914
1873
selectable marker
AmpR
Ampicillin resistance
3054
3914
tag
Flag
N-terminal Flag tag
250
273
tag
His
N-terminal 6xHis tag
292
309
trxn termination sequence
T7 term
T7 terminator
26
72
