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Detailed Vector Information: pMCSG54

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Vector Name: pMCSG54
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Synonyms: None

pMCSG54

Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with a T7 promoter and N-terminal Flag and 6xHiis tag followed by TEV protease cleavage site ; ampicillin resistance in bacteria; ligation independent cloning.
Comments: origin of replication similar to pUC vectors
Size (bp): 4302
Empty Vector: EvNO00454845
Parent Vector: None
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI:Biology
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 origin of replication 2295 2883
ligation independent cloning LIC ligation independent cloning site 204 235
protease cleavage site TEV TEV protease cleavage site 223 243
repressor binding site lac operator LacO fragment 359 381
repressor protein gene LacI Lac I 914 1873
selectable marker AmpR Ampicillin resistance 3054 3914
tag Flag N-terminal Flag tag 250 273
tag His N-terminal 6xHis tag 292 309
trxn termination sequence T7 term T7 terminator 26 72