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Detailed Vector Information: pJSP6_nHalo_empty


Vector Name: pJSP6_nHalo_empty
       DyNA Vector Map

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Synonyms: pEU-nHalo_empty


Sequencing Primer: Forward:  HaloseqF
Reverse:  pF1KseqR
Description: Wheat germ expression vector with SP6 promoter and TEV-cleavable N-terminal HALO tag; ampicillin resistance in bacteria; gateway cloning
Comments: Modified from pEU_HSBC, expresses Halo tag without an insert
Size (bp): 4775
Empty Vector: EvNO00450884
Parent Vector: None
Properties: Gateway, acceptor (destination), cell-free expression, recombinational cloning, with tag/fusion/marker
Author Name: Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Center for Personalized Diagnostics
Publications: None
Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
IRES TMV Omega TMV Omega IRES 9 131
bacterial origin ColE ColE1 bacterial origin 2229 2911
primer reverse primer pF1KseqR reverse sequencing primer CTTTCGGGCTTTGTTAGCAG 1163 1182
primer forward primer HaloseqF forward sequencing primer aagcctgcctaactgcaa 902 919
promoter SP6 SP6 promoter 4759 4775
protease cleavage site TEV TEV protease cleavage site EDLYFQS 1035 1055
selectable marker AmpR Ampicillin resistance 3009 3668
tag HALO N-terminal HALO tag 132 1022
trxn termination sequence T7 term T7 terminator 1132 1145