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Detailed Vector Information: pMCSG73


Vector Name: pMCSG73
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Synonyms: None


Sequencing Primer: Forward:  MCSG73F
Reverse:  T7 terminator
Description: Bacterial expression vector with a T7 promoter and TVMV-cleavable N-terminal NusA tag and TEV-cleavable N-terminal 6xHis and StrepII tag; Contains rate argU and ileX tRNA genes; ampicillin resistance in bacteria; ligation independent cloning.
Comments: None
Size (bp): 6227
Empty Vector: None
Parent Vector: Name: pMCSG53
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 origin of replication 4220 4808
ligation independent cloning LIC ligation independent cloning site 138 16
promoter T7 T7 promoter 1779 1795
protease cleavage site TEV TEV protease cleavage site 155 175
protease cleavage site TVMV TVMV protease cleavage site 218 238
repressor binding site lac operator lac operator 1754 1776
repressor protein gene LacI LacI CDS 2832 3791
selectable marker AmpR ampicillin resistance gene 4979 5839
tRNA ileX ileX tRNA gene 2378 2529
tRNA argU argU tRNA 1862 2218
tag NusA N-terminal NusA 249 1708
tag His N-terminal 6xHis tag 200 217
tag strep N-terminal StrepII tag 176 199
trxn termination sequence T7 term T7 terminator 26 72