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Detailed Vector Information: pGEc2-DEST


Vector Name: pGEc2-DEST
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Synonyms: ''


Sequencing Primer: Forward:  pGEc1-DEST F
Reverse:  pGEn1-DEST R
Description: Mammalian expression vector with a CMV promoter and C-terminal Superfolder GFP, AviTag and 10xHis tag; ampicillin resistance in bacteria; Gateway recombinational cloning
Comments: None
Size (bp): 6852
Empty Vector: EvNO00534627
Parent Vector: Name: pXKM
Properties: Gateway, acceptor (destination), fluorescent marker, mammalian transduction, recombinational cloning, with tag/fusion/marker
Author Name: University of Georgia
Kelley Moremen
Publications: None
Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 origin 1137 1804
death cassette ccdB ccdB death cassette (removed in plasmids with insert) 4211 4516
intron artificial intron artificial intron 2828 2960
poly-A signal BGH pA bovine growth hormone (BGH) polyadenylation signal 6028 6242
promoter CMV CMV promoter for expression in mammalian cells 2208 2790
recombination site 3'-ITR inverted terminal repeat sequence 6330 6459
recombination site attR1 attR1 recombinational cloning site 2974 3101
recombination site attR2 attR2 recombinational cloning site 4557 4681
selectable marker AmpR ampicillin resistance 129 989
selectable marker CmR Chloramphenicol resistance (CmR) gene (removed in plasmids with insert) 3210 3859
tag GFP C-terminal super GFP 4682 5377
tag AviTag C-terminal AviTag 5378 5428
tag His C-terminal 10xHis tag 5429 5452
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 5480 6021