Vector Name:
pJFT7_n3xHA-Halo_DC
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable 3xHA-Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
None
Size (bp):
6313
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning
Author Name:
Arizona State University
Joshua LaBaer
Justin Saul
Ji Qiu
Fernanda Festa
Center for Personalized Diagnostics
Joshua LaBaer
Justin Saul
Ji Qiu
Fernanda Festa
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
M13 Ori
M13 origin
5674
6129
bacterial origin
ColE
ColE1 origin
3598
4280
death cassette
ccdB
Gateway ccdB death Cassette (DC) (removed in plasmids with inserts)
1585
3239
death cassette 2
ccdB (BsrGI removed)
ccdB (bsrgi removed)
2794
3099
enhancer
CITE
CITE enhancer
17
515
primer
M13 forward
M13 forward sequencing primer
6273
6290
primer
forward primer
HaloseqF aagcctgcctaactgcaa (used for sequencing the gene insert)
1391
1408
primer
T7 reverse
T7 term primer (can be used to sequence the insert)
3358
3376
primer
NAP138
NAP138r reverse sequencing primer tgtttcgccatttatcacctt (can be used to sequence the gene insert)
3299
3319
promoter
T7
T7 promoter
6297
6313
protease cleavage site
TEV
TEV protease cleavage site EDLYFQS (used to separate expressed protein from 3xHA-HALO tag)
1524
1544
recombination site
attR2
attR2 recombination site
3240
3264
recombination site
attR1
attR1 recombination site
1560
1584
reporter gene
LacZ
LacZ alpha
6134
6202
selectable marker
AmpR
ampicillin resistance gene
4378
5037
selectable marker
CmR
Chloramphenicol resistance gene (removed in plasmids containing gene inserts)
1793
2449
ssDNA origin
f1
f1 origin
5806
6112
tag
HALO
N-terminal HALO tag
624
1511
tag
HA
N-terminal 3xHA tag
525
617
trxn termination sequence
T7 term
T7 terminator
3369
3416
