Vector Name:
pJFT7_nHA_cHalo_DC
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal HA and C-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
None
Size (bp):
6241
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Justin Saul
Ji Qiu
Fernanda Festa
Justin Saul
Ji Qiu
Fernanda Festa
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3526
4208
bacterial origin
M13 Ori
M13 origin
5602
6057
death cassette
ccdB (BsrGI removed)
ccdB death cassette (removed in plasmids containing an insert)
1801
2106
enhancer
CITE
CITE enhancer
17
515
primer
reverse primer
cHaloseqR reverse sequencing primer CAACATCGACGTAGTGCAT
2387
2405
primer
forward primer
nap150f forward sequencing primer cccattgtatgggatctgatc
388
408
promoter
T7
T7 promoter
6217
3
protease cleavage site
TEV
TEV protease cleavage site (removes HALO tag)
2294
2314
recombination site
attR1
attR1 recombinational cloning site
567
590
recombination site
attR2
attR2 recombinational cloning site
2247
2271
reporter gene
LacZ
LacZ alpha
6062
6130
selectable marker
AmpR
ampicillin resistance
4306
4965
selectable marker
CmR
chloramphenicol resistance (removed in plasmids containing an insert)
800
1356
ssDNA origin
f1
f1 origin
5734
6040
tag
HA
N-terminal HA tag
531
557
tag
HALO
C-terminal HALO tag
2330
3214
trxn termination sequence
T7 term
T7 transcriptional termination
3297
3344
