Detailed Vector Information: pJFT7_nHIS_cHalo_DC

Vector Name:
pJFT7_nHIS_cHalo_DC
Synonyms:
''
Sequencing Primer:
Forward:  NAP150
Reverse:  cHaloseqR
Description:
In vitro expression vector with a T7 promoter and N-terminal HIS and C-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
None
Size (bp):
6232
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Justin Saul
Ji Qiu
Fernanda Festa
Publications:
None
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3511
4193
death cassette
ccdB (BsrGI removed)
ccdB death cassette (removed in plasmids that contain an insert)
1786
2091
enhancer
CITE
CITE enhancer
17
515
primer
reverse primer
cHaloseqR reverse sequencing primer CAACATCGACGTAGTGCAT
2372
2390
primer
forward primer
nap150f forward sequencing primer cccattgtatgggatctgatc
388
408
promoter
T7
T7 promoter
6210
6226
protease cleavage site
TEV
TEV protease cleavage site (removes Halo tag)
2279
2299
recombination site
attR1
attR1 recombinational cloning site
552
576
recombination site
attR2
attR2 recombinational cloning site
2232
2256
selectable marker
AmpR
ampicillin resistance
4291
4950
selectable marker
CmR
chloramphenicol resistance (removed in plasmids containing an insert)
785
1441
ssDNA origin
f1
f1 origin
5719
6025
tag
HALO
C-terminal Halo tag
2315
3199
tag
His
N-terminal 6xHis tag
525
542
trxn termination sequence
T7 term
T7 transcriptional terminator
3282
3329