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Detailed Vector Information: pMCSG59


Vector Name: pMCSG59
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Synonyms: None


Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with T7 promoter and TEV-cleavable C-terminal 6xHis tag; contain argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning
Comments: LIC region plus a portion of the beta-lactamase gene from pMCSG28 removed by digestion with BglI and XbaI then ligated into pMCSG53 digested with the same enzymes. Product is C-terminal His-tag plasmid with truncated ori and the argU & ileX tRNA genes
Size (bp): 4775
Empty Vector: EvNO00599464
Parent Vector: Name: pMCSG53
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
Publications: PMID: 24057978
Title: New LIC vectors for production of proteins from genes containing rare codons

Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 origin of replication 2768 3356
ligation independent cloning LIC ligation independent cloning site 245 265
promoter T7 T7 promoter 327 343
protease cleavage site TEV TEV protease cleavage site 232 252
repressor binding site lac operator lac operator 302 324
repressor binding site LacI lac I cds 1380 2339
selectable marker AmpR ampicillin resistance 3527 4387
tRNA argU argU rare tRNA 410 766
tRNA ileX ileX tRNA gene 926 1077
tag His C-terminal 6xHis tag 208 225
trxn termination sequence T7 term T7 terminator 23 72