Vector Name:
pMCSG59
Synonyms:
''
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector with T7 promoter and TEV-cleavable C-terminal 6xHis tag; contain argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning
Comments:
LIC region plus a portion of the beta-lactamase gene from pMCSG28 removed by digestion with BglI and XbaI then ligated into pMCSG53 digested with the same enzymes. Product is C-terminal His-tag plasmid with truncated ori and the argU & ileX tRNA genes
Size (bp):
4775
Parent Vector:
Name:
pMCSG53
Description:
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
Publications:
PMID:
24057978
Title: New LIC vectors for production of proteins from genes containing rare codons
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR322 origin of replication
2768
3356
ligation independent cloning
LIC
ligation independent cloning site
245
265
promoter
T7
T7 promoter
327
343
protease cleavage site
TEV
TEV protease cleavage site
232
252
repressor binding site
LacI
lac I cds
1380
2339
repressor binding site
lac operator
lac operator
302
324
selectable marker
AmpR
ampicillin resistance
3527
4387
tRNA
ileX
ileX tRNA gene
926
1077
tRNA
argU
argU rare tRNA
410
766
tag
His
C-terminal 6xHis tag
208
225
trxn termination sequence
T7 term
T7 terminator
23
72
