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Detailed Vector Information: pMCSG63


Vector Name: pMCSG63
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Synonyms: ''


Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial co-expresion vector with T7 promoter and N-terminal TEV-cleavable 6xHis tag; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning
Comments: LIC regions plus a portion of the beta-lactamase gene from pMCSG60 removed by digestion with BglI and XbaI then ligated into pMCSG53 ligested with the same enzymes. Product is dual-LIC plasmid with truncated ori and the argU & ileX tRNA genes
Size (bp): 4864
Empty Vector: EvNO00599484
Parent Vector: Name: pMCSG53
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
Publications: PMID: 24057978
Title: New LIC vectors for production of proteins from genes containing rare codons

Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin 2857 3445
ligation independent cloning LIC MCSG7 LIC site 260 291
ligation independent cloning LIC2 MCSG26 LIC site 223 244
promoter T7 T7 promoter 416 432
protease cleavage site TEV TEV protease cleavage site 279 299
repressor binding site lac operator lac operator 391 413
repressor protein gene LacI LacI 1349 2428
ribosome binding site RBS ribosome binding site 243 248
ribosome binding site RBS2 ribosome binding site 353 358
selectable marker AmpR ampicillin resistance 3616 4476
tRNA ileX ileX rare tRNA 1015 1166
tRNA argU argU rare tRNA 499 855
tag His N-terminal 6xHis tag 324 341
trxn termination sequence T7 term T7 terminator 26 72