Vector Name:
pMCSG63
Synonyms:
''
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial co-expresion vector with T7 promoter and N-terminal TEV-cleavable 6xHis tag; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning
Comments:
LIC regions plus a portion of the beta-lactamase gene from pMCSG60 removed by digestion with BglI and XbaI then ligated into pMCSG53 ligested with the same enzymes. Product is dual-LIC plasmid with truncated ori and the argU & ileX tRNA genes
Size (bp):
4864
Parent Vector:
Name:
pMCSG53
Description:
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
Publications:
PMID:
24057978
Title: New LIC vectors for production of proteins from genes containing rare codons
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR322 bacterial origin
2857
3445
ligation independent cloning
LIC
MCSG7 LIC site
260
291
ligation independent cloning
LIC2
MCSG26 LIC site
223
244
promoter
T7
T7 promoter
416
432
protease cleavage site
TEV
TEV protease cleavage site
279
299
repressor binding site
lac operator
lac operator
391
413
repressor protein gene
LacI
LacI
1349
2428
ribosome binding site
RBS
ribosome binding site
243
248
ribosome binding site
RBS2
ribosome binding site
353
358
selectable marker
AmpR
ampicillin resistance
3616
4476
tRNA
ileX
ileX rare tRNA
1015
1166
tRNA
argU
argU rare tRNA
499
855
tag
His
N-terminal 6xHis tag
324
341
trxn termination sequence
T7 term
T7 terminator
26
72
