Vector Name:
pMCSG71
Synonyms:
''
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector with T7 promoter, N-terminal TVMV-cleavable MBP tag and N-terminal TEV-cleavable 6xHis tag; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning
Comments:
LIC region plus a portion of the beta-lactamase gene from pMCSG32 removed by digestion with BglI and XbaI then ligated into pMCSG53 digested with the same enzymes. Product is pMCSG32 with truncated ori and the argU & ileX tRNA genes
Size (bp):
5940
Parent Vector:
Name:
pMCSG53
Description:
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
Publications:
PMID:
24057978
Title: New LIC vectors for production of proteins from genes containing rare codons
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR322 origin of replication
3933
4521
ligation independent cloning
LIC
ligation independent cloning site
245
265
promoter
T7
T7 promoter
1492
1508
protease cleavage site
TEV
TEV protease cleavage site
232
252
protease cleavage site
TVMV
TVMV protease cleavage site
263
283
repressor binding site
lac operator
lac operator
1476
1489
repressor protein gene
LacI
LacI
2545
2504
selectable marker
AmpR
ampicilin resistance
4692
5552
tRNA
ileX
ileX rare tRNA
2091
2242
tRNA
argU
argU rare tRNA
1575
1931
tag
His
C-terminal 6xHis tag
208
225
tag
MBP
N-terminal Maltose binding protein tag
314
1420
