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Detailed Vector Information: pMCSG71

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Vector Name: pMCSG71
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Synonyms: None

pMCSG71

Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with T7 promoter, N-terminal TVMV-cleavable MBP tag and N-terminal TEV-cleavable 6xHis tag; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning
Comments: LIC region plus a portion of the beta-lactamase gene from pMCSG32 removed by digestion with BglI and XbaI then ligated into pMCSG53 digested with the same enzymes. Product is pMCSG32 with truncated ori and the argU & ileX tRNA genes
Size (bp): 5940
Empty Vector: EvNO00599486
Parent Vector: Name: pMCSG53
Description:
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI:Biology
Publications: PMID: 24057978
Title: New LIC vectors for production of proteins from genes containing rare codons

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 origin of replication 3933 4521
ligation independent cloning LIC ligation independent cloning site 245 265
promoter T7 T7 promoter 1492 1508
protease cleavage site TEV TEV protease cleavage site 232 252
protease cleavage site TVMV TVMV protease cleavage site 263 283
repressor binding site lac operator lac operator 1476 1489
repressor protein gene LacI LacI 2545 2504
selectable marker AmpR ampicilin resistance 4692 5552
tRNA argU argU rare tRNA 1575 1931
tRNA ileX ileX rare tRNA 2091 2242
tag MBP N-terminal Maltose binding protein tag 314 1420
tag His C-terminal 6xHis tag 208 225