Vector Name:
pLIFE-3'UTR
Synonyms:
''
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
Mammalian expression vector for detecting the expression of 3'UTRs by Firefly luciferase; also contains Renilla luciferase for normalization; ampicillin resistance in bacteria; Gateway cloning with P2RP3 Gateway Cassette
Comments:
Growth before gateway recombination requires DB3.1 E. coli cells.
Size (bp):
9206
Parent Vector:
None
Properties:
Gateway, acceptor (destination), fluorescent marker, mammalian expression, recombinational cloning
Author Name:
Arizona State University
Marco Mangone
Justin Wolter
Center for Personalized Diagnostics
Marco Mangone
Justin Wolter
Center for Personalized Diagnostics
Publications:
PMID:
25074381
Title: 3pLIFE: a functional assay to detect miRNA targets in high-throughput
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
3pUTR
SV40
SV40 3'UTR
3692
3813
bacterial origin
ColE
ColE1 bacterial origin
1117
1705
binding site
MS2
N-terminal 4xMS2 repeat
6038
6204
fluorescent marker
Renilla Luciferase
Renilla Luciferase
2745
3680
fluorescent marker
Firefly Luciferase
Firefly luciferase
4375
6027
promoter
CMV
CMV promoter driving the expression of the Renilla luciferase
2141
2649
promoter
PGK
PGK promoter driving the expression of the Firefly luciferase and 3'UTR
3854
4352
selectable marker
CmR
Chloramphenicol resistance (part of the P2RP3 Gateway Cassette); removed in plasmids containing a 3'UTR
6725
7381
selectable marker
AmpR
ampicillin resistance
1
946
ssDNA origin
f1
f1 origin
8706
9161
