Detailed Vector Information: pSFV3

Vector Name:
pSFV3
Synonyms:
''
Sequencing Primer:
Forward:  pFAB007
Reverse:  Unknown
Description:
Bacterial expression vector for recombinant antibody expression with a Tac promoter and C-terminal 6xHis tag; ampicillin resistance in bacteria
Comments:
None
Size (bp):
6775
Parent Vector:
None
Properties:
bacterial expression, loxP, with tag/fusion/marker
Author Name:
Structural Genomics Consortium
Publications:
None
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR322 origin
1612
2231
gene fragment
heavy chain
5625
6470
gene fragment
light chain
Light chain
4796
5506
promoter
lac
lac promoter
3171
3200
promoter
lac 2
Second lac promoter
4497
4520
promoter
tac promoter
Tac promoter
4607
4635
promoter
tac promoter 2
Second Tav promoter
4702
4730
recombination site
LoxP
Lox P site
4908
4938
reporter gene
LacZ
Lac Z reporter gene
2950
3109
repressor protein gene
LacI
Lac I
3249
4340
selectable marker
AmpR
ampicillin resistance
597
1457
selectable marker
sacB
Sac B gene allowing negative selection on 5% sucrose
4947
4882
ssDNA origin
f1
f1 origin
29
335
tag
His
C-terminal 6xHis tag
6535
6615
trxn termination sequence
TrpA
TrpA transcription terminator
6657
6684