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Detailed Vector Information: pSFV3


Vector Name: pSFV3
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Synonyms: ''


Sequencing Primer: Forward:  pFAB007
Reverse:  Unknown
Description: Bacterial expression vector for recombinant antibody expression with a Tac promoter and C-terminal 6xHis tag; ampicillin resistance in bacteria
Comments: None
Size (bp): 6775
Empty Vector: None
Parent Vector: None
Properties: bacterial expression, loxP, with tag/fusion/marker
Author Name: Structural Genomics Consortium
Publications: None
Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 origin 1612 2231
gene fragment heavy chain 5625 6470
gene fragment light chain Light chain 4796 5506
promoter lac lac promoter 3171 3200
promoter lac 2 Second lac promoter 4497 4520
promoter tac promoter Tac promoter 4607 4635
promoter tac promoter 2 Second Tav promoter 4702 4730
recombination site LoxP Lox P site 4908 4938
reporter gene LacZ Lac Z reporter gene 2950 3109
repressor protein gene LacI Lac I 3249 4340
selectable marker AmpR ampicillin resistance 597 1457
selectable marker sacB Sac B gene allowing negative selection on 5% sucrose 4947 4882
ssDNA origin f1 f1 origin 29 335
tag His C-terminal 6xHis tag 6535 6615
trxn termination sequence TrpA TrpA transcription terminator 6657 6684