Detailed Vector Information: pCW-FABAVI-LIC

Vector Name:
pCW-FABAVI-LIC
Synonyms:
''
Sequencing Primer:
Forward:  pFAB007
Reverse:  Unknown
Description:
Recombinant antibody expression vector with Avi-tagged heavy chain; ampicillin resistance in bacteria
Comments:
None
Size (bp):
6637
Parent Vector:
None
Properties:
bacterial expression, with tag/fusion/marker
Author Name:
Structural Genomics Consortium
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
DNA Vector Map
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Vector Features:

Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR322 bacterial origin
1612
2231
gene fragment
light chain
Light chain, PelB signal sequence
4796
5506
gene fragment
heavy chain
Heavy chain
5625
6470
promoter
lac
lac promoter
3171
3200
promoter
lac 2
second lac promoter
4497
4520
promoter
tac promoter
tac promoter
4607
4635
promoter
tac promoter 2
Second tac promoter
4702
4730
repressor protein gene
LacI
LacI
3249
4340
selectable marker
AmpR
Ampicillin resistance
597
1454
ssDNA origin
f1
f1 origin
29
335
tag
AviTag
Avi tagged heavy chain
6426
6470
trxn termination sequence
TrpA
TrpA transcription terminator
6519
6546