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Detailed Vector Information: pCW-FABAVI-LIC


Vector Name: pCW-FABAVI-LIC
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Synonyms: ''


Sequencing Primer: Forward:  pFAB007
Reverse:  Unknown
Description: Recombinant antibody expression vector with Avi-tagged heavy chain; ampicillin resistance in bacteria
Comments: None
Size (bp): 6637
Empty Vector: None
Parent Vector: None
Properties: bacterial expression, with tag/fusion/marker
Author Name: Structural Genomics Consortium
Publications: None
Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin 1612 2231
gene fragment light chain Light chain, PelB signal sequence 4796 5506
gene fragment heavy chain Heavy chain 5625 6470
promoter lac lac promoter 3171 3200
promoter lac 2 second lac promoter 4497 4520
promoter tac promoter tac promoter 4607 4635
promoter tac promoter 2 Second tac promoter 4702 4730
repressor protein gene LacI LacI 3249 4340
selectable marker AmpR Ampicillin resistance 597 1454
ssDNA origin f1 f1 origin 29 335
tag AviTag Avi tagged heavy chain 6426 6470
trxn termination sequence TrpA TrpA transcription terminator 6519 6546