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Detailed Vector Information: pJSP6_cHalo_DC

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Vector Name: pJSP6_cHalo_DC
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Synonyms: None

pJSP6_cHalo_DC

Sequencing Primer: Forward:  SP6
Reverse:  cHaloseqR
Description: Wheat germ expression vector with SP6 promoter and TEV-cleavable C-terminal HALO tag; ampicillin resistance in bacteria; gateway cloning
Comments: None
Size (bp): 6502
Empty Vector: EvNO00623724
Parent Vector: None
Properties: Gateway, acceptor (destination), cell-free expression, recombinational cloning, with tag/fusion/marker
Author Name: Joshua LaBaer
Center for Personalized Diagnostics
Arizona State University
Ji Qiu
Justin Saul
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin 3956 4638
death cassette ccdB ccdB death cassette (removed in plasmids containing a gene insert) 1471 1680
enhancer TMV Omega TMV Omega enhancer 9 129
protease cleavage site TEV TEV protease cleavage site 1871 1891
recombination site attR1 attR1 recombination site 144 160
recombination site attR2 attR2 recombinational cloning site 1831 1846
selectable marker AmpR ampicillin resistance 4736 5395
selectable marker CmR Chloramphenicol resistance (removed in plasmids containing a gene insert) 377 1033