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Detailed Vector Information: pET-32b-TEV

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Vector Name: pET-32b-TEV
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Synonyms: None

pET-32b-TEV

Sequencing Primer: Forward:  TRXF
Reverse:  T7 terminator
Description: Bacterial expression vector with T7 promoter and N-terminal E. coli thioredoxin tag, 6xHis-tag, thrombin cleavage site, S-tag, and TEV cleavage site; ampicillin resistance in bacteria; restriction enzyme cloning
Comments: Clone insert in-frame using the BamHI and HindIII restriction sites
Size (bp): 5905
Empty Vector: EvNO00629003
Parent Vector: None
Properties: bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name: PSI:Biology
Arizona State University
Center for Membrane Proteins in Infectious Diseases
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 origin 1554 2236
promoter T7 T7 promoter 5121 5137
protease cleavage site TEV TEV protease cleavage site 5668 5688
protease cleavage site Thrombin thrombin protease cleavage site 5584 5601
repressor binding site lac operator lac operator 5140 5164
repressor protein gene LacI LacI 3651 4730
selectable marker AmpR ampicillin resistance 599 1456
ssDNA origin f1 f1 origin 12 467
tag His N-terminal 6xHis tag 5749 5766
tag S-tag N-terminal S-tag 5608 5652
tag TRX N-terminal E.Coli thioredoxin tag 5209 5535
trxn termination sequence T7 term T7 terminator 5834 5880