Detailed Vector Information: pET-32b-TEV

Vector Name:
pET-32b-TEV
Synonyms:
''
Sequencing Primer:
Forward:  TRXF
Reverse:  T7 terminator
Description:
Bacterial expression vector with T7 promoter and N-terminal E. coli thioredoxin tag, 6xHis-tag, thrombin cleavage site, S-tag, and TEV cleavage site; ampicillin resistance in bacteria; restriction enzyme cloning
Comments:
Clone insert in-frame using the BamHI and HindIII restriction sites
Size (bp):
5905
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name:
Arizona State University
PSI:Biology
Center for Membrane Proteins in Infectious Diseases
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
DNA Vector Map
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Vector Features:

Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
1554
2236
promoter
T7
T7 promoter
5121
5137
protease cleavage site
Thrombin
thrombin protease cleavage site
5584
5601
protease cleavage site
TEV
TEV protease cleavage site
5668
5688
repressor binding site
lac operator
lac operator
5140
5164
repressor protein gene
LacI
LacI
3651
4730
selectable marker
AmpR
ampicillin resistance
599
1456
ssDNA origin
f1
f1 origin
12
467
tag
His
N-terminal 6xHis tag
5749
5766
tag
S-tag
N-terminal S-tag
5608
5652
tag
TRX
N-terminal E.Coli thioredoxin tag
5209
5535
trxn termination sequence
T7 term
T7 terminator
5834
5880