Vector Name:
pBAD24sigNtermHis
Synonyms:
''
Description:
Arabinose-inducible bacterial expression with a pBAD promoter and N-terminal E. coli YtfM (aa1-21) and 6xHis-tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
Comments:
Signal sequence is aa 1-21 from E. coli YtfM, which was supposedly used in PMID 20060837. Unfortunately, the actual sequence used in this publication was actually YtfM aa 1-27 with the V20A mutation, followed by 6xHis + LE (van den Berg, personal communication)
Size (bp):
5013
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Arizona State University
Felicia M. Craciunescu
Center for Membrane Proteins in Infectious Diseases
PSI:Biology
Felicia M. Craciunescu
Center for Membrane Proteins in Infectious Diseases
PSI:Biology
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR322 origin
3719
4416
promoter
pBAD
pBad promoter
1250
1277
repressor binding site
arabinose O2 operator
arabinose O2 operator
1003
1020
repressor binding site
arabinose O1 operator
arabinose O1 operator
1161
1182
repressor protein gene
araC
araC
96
974
ribosome binding site
RBS
ribosome binding site
1306
1311
selectable marker
AmpR
ampicillin resistance
2356
3219
signal sequence
YtfM
N-terminal E. coli YtfM (aa1-21) signal sequence
1320
1382
tag
His
N-terminal 6xHis tag
1383
1400
trxn termination sequence
rrnB
rrnB transcription terminsator
1838
2263
