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Detailed Vector Information: pBAD24sigNtermHis

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Vector Name: pBAD24sigNtermHis
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Synonyms: None

pBAD24sigNtermHis

Sequencing Primer: Forward:  PBADFOR
Reverse:  RRNBTER
Description: Arabinose-inducible bacterial expression with a pBAD promoter and N-terminal E. coli YtfM (aa1-21) and 6xHis-tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
Comments: Signal sequence is aa 1-21 from E. coli YtfM, which was supposedly used in PMID 20060837. Unfortunately, the actual sequence used in this publication was actually YtfM aa 1-27 with the V20A mutation, followed by 6xHis + LE (van den Berg, personal communication)
Size (bp): 5013
Empty Vector: EvNO00629007
Parent Vector: None
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: PSI:Biology
Arizona State University
Center for Membrane Proteins in Infectious Diseases
Felicia M. Craciunescu
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 origin 3719 4416
promoter pBAD pBad promoter 1250 1277
repressor binding site arabinose O2 operator arabinose O2 operator 1003 1020
repressor binding site arabinose O1 operator arabinose O1 operator 1161 1182
repressor protein gene araC araC 96 974
ribosome binding site RBS ribosome binding site 1306 1311
selectable marker AmpR ampicillin resistance 2356 3219
signal sequence YtfM N-terminal E. coli YtfM (aa1-21) signal sequence 1320 1382
tag His N-terminal 6xHis tag 1383 1400
trxn termination sequence rrnB rrnB transcription terminsator 1838 2263