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Detailed Vector Information: pBAD24sigCtermHis

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Vector Name: pBAD24sigCtermHis
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Synonyms: None

pBAD24sigCtermHis

Sequencing Primer: Forward:  PBADFOR
Reverse:  HGHTER
Description: Arabinose-inducible expression in bacteria with N-terminal E. coli YtfM (aa1-21) signal sequence and C-terminal His-tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
Comments: Signal sequence is aa 1-21 from E. coli YtfM, which was supposedly used in PMID 20060837. Unfortunately, the actual sequence used in this publication was actually YtfM aa 1-27 with the V20A mutation, followed by 6xHis + LE (van den Berg, personal communication)
Size (bp): 5017
Empty Vector: EvNO00629008
Parent Vector: None
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: PSI:Biology
Arizona State University
Center for Membrane Proteins in Infectious Diseases
Felicia M. Craciunescu
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 origin 3723 4420
promoter pBAD pBAD promoter 1250 1277
repressor binding site arabinose O2 operator arabinose O2 operator 1003 1020
repressor binding site arabinose O1 operator arabinose O1 operator 1161 1182
repressor protein gene araC araC 96 974
ribosome binding site RBS ribosomal binding site 1306 1311
selectable marker AmpR ampicillin resistance 2360 3223
signal sequence YtfM N-terminal E. coli YtfM (aa1-21) signal sequence 1323 1382
tag His C-terminal 6xHis tag 1814 1831
trxn termination sequence rrnB rrnB termination 1842 2267