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Detailed Vector Information: pRSET-C6xHis


Vector Name: pRSET-C6xHis
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Synonyms: ''


Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with T7 promoter and C-terminal 6xHis tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
Comments: ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
Size (bp): 3242
Empty Vector: EvNO00629011
Parent Vector: None
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Arizona State University
Felicia M. Craciunescu
Center for Membrane Proteins in Infectious Diseases
Publications: None
Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 origin 2342 3024
promoter T7 T7 promoter 20 39
ribosome binding site RBS Ribosome binding site 85 92
selectable marker AmpR ampicillin resistance 1387 2247
ssDNA origin f1 f1 origin 801 1256
tag His C-terminal 6xHis tag 532 549
trxn termination sequence T7 term T7 terminator 601 730