Detailed Vector Information: pRSET-C6xHis

Vector Name:
pRSET-C6xHis
Synonyms:
''
Sequencing Primer:
Forward:  T7
Reverse:  T7 terminator
Description:
Bacterial expression vector with T7 promoter and C-terminal 6xHis tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
Comments:
ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
Size (bp):
3242
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Arizona State University
Felicia M. Craciunescu
Center for Membrane Proteins in Infectious Diseases
PSI:Biology
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
DNA Vector Map
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Vector Features:

Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
2342
3024
promoter
T7
T7 promoter
20
39
ribosome binding site
RBS
Ribosome binding site
85
92
selectable marker
AmpR
ampicillin resistance
1387
2247
ssDNA origin
f1
f1 origin
801
1256
tag
His
C-terminal 6xHis tag
532
549
trxn termination sequence
T7 term
T7 terminator
601
730