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Detailed Vector Information: pPPL

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Vector Name: pPPL
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Synonyms: None

pPPL

Sequencing Primer: Forward:  Unknown
Reverse:  Unknown
Description: ligation independent cloning (LIC) clone with pBR322 origin and Ampicillin Resistance and ROP and ORF frame 2 Tetracycline resistance and ORF frame 1 and pBRrevBam and T7 and T7 term primer and BseRI Stuffer and MBP signal sequence (M1-A26 of NP_290668) and RBS and MBP (K27-T392 of NP_290668) and HIS10 and TEV and FC sequencing primer pET26 pelB seq R and FC sequencing primer MBP pelB seqF and FC sequencing primer pET-PPL seq 1 F and FC sequencing primer pET-PPL seq 1 R and FC sequencing primer pET-PPL seq 2 F and T7 term and FC sequencing primer pET-PPL seq 2 R and AmpR; ampicillin resistance in bacterial;
Comments: TEV protease cleavage site is designed for use with ligation-independent cloning into the BseRI site. This TEV site contains only the amino acids DNLYFQ. Cleavage occurs after the Q, and requires a compatible amino acid C-terminal to the Q, such as S, G, A, M. See Kapust et al 2002 Biochem Biophys Res Commun 294:949
Size (bp): 6447
Empty Vector: EvNO00813763
Parent Vector: Name: pET PPL His6 MBP LIC cloning vector (2K-T)
Description:
Properties: ligation independent cloning (LIC)
Author Name: Debra T. Hansen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
CDS ORF frame 1 3283 3741
CDS BseRI Stuffer 5456 5867
CDS Ampicillin Resistance 209 1069
CDS ORF frame 2 Tetracycline resistance 3088 3873
Miscellaneous Feature MBP signal sequence (M1-A26 of NP_290668) 4147 4224
Miscellaneous Feature ROP 2258 2449
Miscellaneous Feature TEV 5428 5445
Miscellaneous Feature MBP (K27-T392 of NP_290668) 4276 5373
Replication Origin pBR322 origin 1224 184
Terminator T7 term 5941 6069
primer T7 term primer 5983 6001
primer pBRrevBam 3907 3926
primer FC sequencing primer pET-PPL seq 2 R 6102 6122
primer FC sequencing primer pET-PPL seq 2 F 4678 4702
primer FC sequencing primer pET-PPL seq 1 R 5943 5964
primer FC sequencing primer pET-PPL seq 1 F 3979 4000
primer FC sequencing primer pET26 pelB seq R 6026 6047
primer FC sequencing primer MBP pelB seqF 5250 5274
promoter T7 4010 4028
promoter AmpR 139 167
ribosome binding site RBS 4130 4139
tag HIS10 4234 4263