Vector Name:
pPPL
Synonyms:
''
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
ligation independent cloning (LIC) clone with pBR322 origin and Ampicillin Resistance and ROP and ORF frame 2 – Tetracycline resistance and ORF frame 1 and pBRrevBam and T7 and T7 term primer and BseRI Stuffer and MBP signal sequence (M1-A26 of NP_290668) and RBS and MBP (K27-T392 of NP_290668) and HIS10 and TEV and FC sequencing primer pET26 pelB seq R and FC sequencing primer MBP pelB seqF and FC sequencing primer pET-PPL seq 1 F and FC sequencing primer pET-PPL seq 1 R and FC sequencing primer pET-PPL seq 2 F and T7 term and FC sequencing primer pET-PPL seq 2 R and AmpR; ampicillin resistance in bacterial;
Comments:
TEV protease cleavage site is designed for use with ligation-independent cloning into the BseRI site. This TEV site contains only the amino acids DNLYFQ. Cleavage occurs after the Q, and requires a compatible amino acid C-terminal to the Q, such as S, G, A, M. See Kapust et al 2002 Biochem Biophys Res Commun 294:949
Size (bp):
6447
Parent Vector:
Name:
pET PPL His6 MBP LIC cloning vector (2K-T)
Description:
Properties:
ligation independent cloning (LIC)
Author Name:
Debra T. Hansen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
Ampicillin Resistance
209
1069
CDS
ORF frame 2 – Tetracycline resistance
3088
3873
CDS
ORF frame 1
3283
3741
CDS
BseRI Stuffer
5456
5867
Miscellaneous Feature
ROP
2258
2449
Miscellaneous Feature
MBP signal sequence (M1-A26 of NP_290668)
4147
4224
Miscellaneous Feature
MBP (K27-T392 of NP_290668)
4276
5373
Miscellaneous Feature
TEV
5428
5445
Replication Origin
pBR322 origin
1224
184
Terminator
T7 term
5941
6069
primer
pBRrevBam
3907
3926
primer
T7 term primer
5983
6001
primer
FC sequencing primer pET26 pelB seq R
6026
6047
primer
FC sequencing primer MBP pelB seqF
5250
5274
primer
FC sequencing primer pET-PPL seq 1 F
3979
4000
primer
FC sequencing primer pET-PPL seq 1 R
5943
5964
primer
FC sequencing primer pET-PPL seq 2 F
4678
4702
primer
FC sequencing primer pET-PPL seq 2 R
6102
6122
promoter
AmpR
139
167
promoter
T7
4010
4028
ribosome binding site
RBS
4130
4139
tag
HIS10
4234
4263
