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Detailed Vector Information: pelB-MBP

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Vector Name: pelB-MBP
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Synonyms: None

pelB-MBP

Sequencing Primer: Forward:  MBPpelBseqF
Reverse:  T7 terminator
Description: ligation independent cloning (LIC) clone, bacterial expression expression with RBS (extended) and TET-2 and pelB and pBR322 origin and KanR and ROP and BseRI and HIS10 and MBP and TEV and FC sequencing primer pET26 pelB seq R and FC sequencing primer MBP pelB seqF and TET and LacI and FC sequencing primer pET-PPL seq 1 F and FC sequencing primer pET-PPL seq 1 R and FC sequencing primer pET-PPL seq 2 F and pBRrevBam and T7 and lac operator and T7 term primer; kanamycin resistance in bacterial;
Comments: TEV protease cleavage site is designed for use with ligation-independent cloning into the BseRI site. This TEV site contains only the amino acids DNLYFQ. Cleavage occurs after the Q, and requires a compatible amino acid C-terminal to the Q, such as S, G, A, M. See Kapust et al 2002 Biochem Biophys Res Commun 294:949
Size (bp): 6932
Empty Vector: EvNO00813783
Parent Vector: Name: pET26-MBP-ELIC
Description:
Properties: bacterial expression, ligation independent cloning (LIC)
Author Name: Dr. Debbie Hansen
Felicia Craciunescu
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
Stuffer BseRI 6339 6770
bacterial origin pBR322 origin 1482 2101
copy control gene ROP 2516 2707
primer FC sequencing primer MBP pelB seqF 6118 6212
primer T7 term primer 6847 6865
primer pBRrevBam 4882 4901
primer FC sequencing primer pET-PPL seq 2 F 5616 5640
primer FC sequencing primer pET-PPL seq 1 R 6807 6828
primer FC sequencing primer pET-PPL seq 1 F 4954 4975
primer FC sequencing primer pET26 pelB seq R 6890 6911
promoter lac operator 5003 5030
promoter T7 4985 5003
protease cleavage site TEV 6321 6338
repressor protein gene LacI 3516 4607
ribosome binding site RBS (extended) 5048 5064
selectable marker TET 611 336
selectable marker KanR 561 1376
selectable marker TET-2 4699 4905
signal sequence pelB pelB signal sequence (cleavage after QPAMA), BseRI removed 5073 5138
tag MBP K27-T392 from NP_290668 5214 6311
tag HIS10 5178 5207