Vector Name:
pelB-MBP
Synonyms:
''
Sequencing Primer:
Forward:
MBPpelBseqF
Reverse:
T7 terminator
Description:
ligation independent cloning (LIC) clone, bacterial expression expression with RBS (extended) and TET-2 and pelB and pBR322 origin and KanR and ROP and BseRI and HIS10 and MBP and TEV and FC sequencing primer pET26 pelB seq R and FC sequencing primer MBP pelB seqF and TET and LacI and FC sequencing primer pET-PPL seq 1 F and FC sequencing primer pET-PPL seq 1 R and FC sequencing primer pET-PPL seq 2 F and pBRrevBam and T7 and lac operator and T7 term primer; kanamycin resistance in bacterial;
Comments:
TEV protease cleavage site is designed for use with ligation-independent cloning into the BseRI site. This TEV site contains only the amino acids DNLYFQ. Cleavage occurs after the Q, and requires a compatible amino acid C-terminal to the Q, such as S, G, A, M. See Kapust et al 2002 Biochem Biophys Res Commun 294:949
Size (bp):
6932
Parent Vector:
Name:
pET26-MBP-ELIC
Description:
Properties:
bacterial expression, ligation independent cloning (LIC)
Author Name:
Dr. Debbie Hansen
Felicia Craciunescu
Felicia Craciunescu
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
Stuffer
BseRI
6339
6770
bacterial origin
pBR322 origin
1482
2101
copy control gene
ROP
2516
2707
primer
pBRrevBam
4882
4901
primer
T7 term primer
6847
6865
primer
FC sequencing primer pET26 pelB seq R
6890
6911
primer
FC sequencing primer MBP pelB seqF
6118
6212
primer
FC sequencing primer pET-PPL seq 1 F
4954
4975
primer
FC sequencing primer pET-PPL seq 1 R
6807
6828
primer
FC sequencing primer pET-PPL seq 2 F
5616
5640
promoter
T7
4985
5003
promoter
lac operator
5003
5030
protease cleavage site
TEV
6321
6338
repressor protein gene
LacI
3516
4607
ribosome binding site
RBS (extended)
5048
5064
selectable marker
KanR
561
1376
selectable marker
TET
611
336
selectable marker
TET-2
4699
4905
signal sequence
pelB
pelB signal sequence (cleavage after QPAMA), BseRI removed
5073
5138
tag
HIS10
5178
5207
tag
MBP
K27-T392 from NP_290668
5214
6311
