Vector Name:
pET-BAD
Synonyms:
''
Sequencing Primer:
Forward:
ARAC5R
Reverse:
T7 terminator
Description:
ligation independent cloning (LIC) clone, bacterial expression expression with Operator O2 and araC promoter and T7 term and Operator O1 and B-lactamase gene and CAP site and T7 term primer and pBR322 origin and Operator I1 and I2 and arabinose BAD promoter and rrnB T1 T2 and RBS and ARAC3F Primer and PCBSREV Primer and ORICOL Primer and BADT1A Primer and BADT1B Primer and AMP5R Primer and -35 AP and F1ORI Primer and ARAC5R and -10 AP and rep_origin and ROP and araC; ampicillin resistance in bacterial;
Comments:
TEV protease cleavage site is designed for use with ligation-independent cloning into the BseRI site. This TEV site contains only the amino acids DNLYFQ. Cleavage occurs after the Q, and requires a compatible amino acid C-terminal to the Q, such as S, G, A, M. See Kapust et al 2002 Biochem Biophys Res Commun 294:949
Size (bp):
4968
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC)
Author Name:
Debra T. Hansen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
-10 signal
-10 AP
3192
3198
-35 signal
-35 AP
3216
3221
CDS
ROP
4342
4533
CDS
B-lactamase gene
2290
3150
CDS
araC
102
980
Miscellaneous Feature
Operator O2
1009
1026
Miscellaneous Feature
Operator O1
1167
1188
Miscellaneous Feature
CAP site
1210
1223
Miscellaneous Feature
Operator I1 and I2
1219
1257
Miscellaneous Feature
RBS
1311
1320
Replication Origin
rep_origin
1721
2168
Terminator
T7 term
1636
1683
Terminator
rrnB T1 T2
1349
1580
primer
T7 term primer
1622
1640
primer
ARAC3F Primer
111
131
primer
PCBSREV Primer
4731
4750
primer
ORICOL Primer
3937
3957
primer
BADT1A Primer
366
385
primer
BADT1B Primer
823
842
primer
AMP5R Primer
3088
3107
primer
F1ORI Primer
1725
1744
primer
ARAC5R
957
976
promoter
araC promoter
1131
1159
promoter
arabinose BAD promoter
1256
1283
rep_origin
pBR322 origin
(pMBI,ColE1)
3298
3971
