Vector Name:
pRSF-BAD
Synonyms:
''
Sequencing Primer:
Forward:
ARAC5R
Reverse:
T7 terminator
Description:
ligation independent cloning (LIC) clone, bacterial expression expression with Kan-R and T7TER primer (#35) and T7 Terminator and RNA II and RNA I and RSF ori - RSF1030 Homology and -10 RNA I and -35 RNA I and -35 AP and -10 AP and RBS-reverse-weak and araC and Operator O2 and araC promoter and Operator O1 and CAP site and Operator I1 and I2 and arabinose BAD promoter and rrnB T1 T2 and RBS and ligation junction Xbal and BADT1A Primer and BADT1B Primer and ARAC3F primer (#145) and ARAC5R primer (#148) and wrong primer #62 off by 3 nt and KAN1F primer (#120) and ligation junction Pacl; 50ug/mL of Kanamycin resistance in bacterial;
Comments:
TEV protease cleavage site is designed for use with ligation-independent cloning into the BseRI site. This TEV site contains only the amino acids DNLYFQ. Cleavage occurs after the Q, and requires a compatible amino acid C-terminal to the Q, such as S, G, A, M. See Kapust et al 2002 Biochem Biophys Res Commun 294:949
Size (bp):
3600
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC)
Author Name:
Dr. Debbie Hansen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
-10 signal
-10 RNA I
3420
3425
-10 signal
-10 AP
2777
2783
-35 signal
-35 RNA I
3396
3401
-35 signal
-35 AP
2800
2805
CDS
Kan-R
1920
2735
CDS
araC
109
987
Miscellaneous Feature
Operator O2
1016
1033
Miscellaneous Feature
Operator O1
1174
1195
Miscellaneous Feature
CAP site
1217
1230
Miscellaneous Feature
Operator I1 and I2
1226
1264
Miscellaneous Feature
RBS
1318
1327
Miscellaneous Feature
ligation junction Xbal
3595
3600
Miscellaneous Feature
ligation junction Pacl
1606
1613
Miscellaneous RNA
RNA II
2843
3541
Miscellaneous RNA
RNA I
3432
3538
RBS
RBS-reverse-weak
2741
2749
Terminator
T7 Terminator
1643
1690
Terminator
rrnB T1 T2
1356
1587
primer
T7TER primer (#35)
1629
1647
primer
BADT1A Primer
373
392
primer
BADT1B Primer
830
849
primer
ARAC3F primer (#145)
118
138
primer
ARAC5R primer (#148)
964
983
primer
wrong primer #62 off by 3 nt
1275
1296
primer
KAN1F primer (#120)
1929
1950
promoter
araC promoter
1138
1166
promoter
arabinose BAD promoter
1263
1290
rep_origin
RSF ori - RSF1030 Homology
2843
3592
