Detailed Vector Information: pPSU2

Vector Name:
pPSU2
Synonyms:
''
Sequencing Primer:
Forward:  None
Reverse:  None
Description:
High copy number plasmid which provides 750, 3000 and 4000 bp EcoRV fragments of kilobase DNA ladder, and 50, 100, 200, 300, 400, 500, 600, 1500 and 4100 bp PstI fragments of 100 bp DNA ladder. Confers ampicillin resistance.
Comments:
Published in Nature Scientific Reports, this plasmid produces DNA molecular weight reference markers when digested with common restriction enzymes. Usually used with pPSU1.Map of the key restriction sites
Standard protocol for using this plasmid
Size (bp):
7750
Parent Vector:
Name: pUC9
Description:
Properties:
high copy number bacterial plasmid
Author Name:
Song Tan
Publications:
PMID: 28550309
Title: The pPSU Plasmids for Generating DNA Molecular Weight Markers
Vector Map:
Vector Sequence:
Sequence:
DNA Vector Map
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Vector Features:

Type
Name
Description
Start
End
restriction site
EcoRV 750bp
750 BP Fragment after EcoRV digestion
241
991
restriction site
EcoRV 3kb
3000 BP Fragment after EcoRV Digestion
991
3991
restriction site
EcoRV 4kb
4000 bp Fragment after EcoRV Digestion
3991
241
restriction site
PstI 1.5kb
1500 bp fragment after PstI digestion
331
1831
restriction site
PstI 50bp
50 bp fragment after PstI digestion
1831
1881
restriction site
PstI 100bp
100 bp fragment after PstI digestion
1881
1981
restriction site
PstI 200bp
200 bp fragment after PstI digestion
1981
2181
restriction site
PstI 300bp
300 bp fragment after PstI Digestion
2181
2481
restriction site
PstI 400bp
400 bp fragment after PstI digestion
2481
2881
restriction site
PstI 500bp
500 bp fragment after PstI digestion
2881
3381
restriction site
PstI 600bp
600 bp fragment after PstI digestion
3381
3981
restriction site
PstI 4100 bp
4100 bp fragment after PstI digestion
3981
331