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This is a list of clones without gene inserts (empty vectors). Please log in to see all empty vectors available to you. Many other vectors are represented in the repository, which can be found using using one of our other search options or in our list of all empty vectors.

Explanation of Terms


  Clone ID Vector Name Description Special
MTA
 
1 EvNO00023103 Plasmid Details pANT7_cGST with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
2 EvNO00023110 Plasmid Details pANT7_nHA In vitro expression vector, makes N-term HA fusion; amp resistance; recombinational cloning.
3 EvNO00023115 Plasmid Details pJP1563 Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (blasticidin for mammalian selection); recombinational cloning.
4 EvNO00084258 Plasmid Details pMCSG10 with tag/fusion/marker assay, multiple cloning site clone, bacterial expression, improves solubility of insert expression with lacI and LIC and AmpR and T7 and T7 term and ori and TEV and His and His 2 and GST; ampicillin resistance in bacterial; PSI
5 EvNO00084259 Plasmid Details pMCSG11 Coexpression vector with T7 promoter, adds N-terminal His tag, TEV, SNA; chloramphenicol resistance; ligation independent cloning (LIC). PSI
6 EvNO00084260 Plasmid Details pMCSG12 Coexpression vector with T7 promoter, adds N-terminal His tag, Sloop, TEV, SNA; chloramphenicol resistance; ligation independent cloning (LIC). PSI
7 EvNO00084261 Plasmid Details pMCSG13 Coexpression vector with T7 promoter, adds N-terminal His tag, MBP, TEV, SNA; chloramphenicol resistance; ligation independent cloning (LIC). PSI
8 EvNO00084262 Plasmid Details pMCSG14 Coexpression vector with T7 promoter, adds N-terminal His tag, GST, TEV, SNA; chloramphenicol resistance; ligation independent cloning (LIC). PSI
9 EvNO00084263 Plasmid Details pMCSG17 Coexpression vector with T7 promoter, adds N-terminal Stag, TEV, SNA; amp resistance; ligation independent cloning (LIC). PSI
10 EvNO00084264 Plasmid Details pMCSG18 Screening vector with T7 promoter, adds N-terminal His tag, TEV, LICs, GFP; amp resistance; ligation independent cloning (LIC). PSI
11 EvNO00084265 Plasmid Details pMCSG19 Vector for solubility of insert and for purification, with T7 promoter, adds N-terminal MBP, TVMV, His, TEV, NA; amp resistance; ligation independent cloning (LIC). PSI
12 EvNO00084266 Plasmid Details pMCSG20 Coexpression vector with T7 promoter, adds N-terminal Stag, GST, TEV, SNA; amp resistance; ligation independent cloning (LIC PSI
13 EvNO00084267 Plasmid Details pMCSG21 Coexpression vector with T7 promoter, adds N-terminal His, TEV, SNA; spectinomycin resistance; ligation independent cloning (LIC). PSI
14 EvNO00084268 Plasmid Details pMCSG22 Coexpression vector with T7 promoter, adds N-terminal His, Sloop, TEV, SNA; spectinomycin resistance; ligation independent cloning (LIC). PSI
15 EvNO00084269 Plasmid Details pMCSG23 Coexpression vector with T7 promoter, adds N-terminal His, MBP, TEV, SNA; spectinomycin resistance; ligation independent cloning (LIC). PSI
16 EvNO00084270 Plasmid Details pMCSG24 Coexpression vector with T7 promoter, adds N-terminal His, GST, TEV, SNA; spectinomycin resistance; ligation independent cloning (LIC). PSI
17 EvNO00084271 Plasmid Details pMCSG7 with tag/fusion/marker assay, ligation independent cloning SspI (LIC-SspI), bacterial expression, in vitro transcription expression with AmpR and His and LIC and ori and T7 and T7 term and TEV and His 2 and LacI and lac operator; ampicillin resistance in bacterial; PSI
18 EvNO00084272 Plasmid Details pMCSG8 Vector facillitating reduced protein toxicity, with T7 promoter, adds N-terminal His tag, Sloop, TEV, SNA; amp resistance; ligation independent cloning (LIC). PSI
19 EvNO00084273 Plasmid Details pMCSG9 Vector with tags for solubility of insert, with T7 promoter, adds N-terminal His tag, MBP, TEV, SNA; amp resistance; ligation independent cloning (LIC). PSI
20 EvNO00084274 Plasmid Details pMCSG19C Vector for solubility of insert and for purification, with T7 promoter, adds N-terminal MBP, TVMV, His, TEV, NA; amp resistance; ligation independent cloning (LIC). PSI
21 EvNO00084275 Plasmid Details pMCentr1 cloning vector to link to Gateway system, with attL1-TEV-LIC site-attL2; zeocin resistance; ligation independent cloning to make pENTR vector for recombinational cloning PSI
22 EvNO00084276 Plasmid Details pMCentr2 cloning vector to link to Gateway system, with attL1-TEV-LIC site-attL2; zeocin resistance; ligation independent cloning to make pENTR vector for recombinational cloning PSI
23 EvNO00084277 Plasmid Details pMCentr3 destination vector to accept inserts from pENTR clones; attL1-TVMV-LIC site-attL2; kanamycin resistance; ligation independent cloning to Gateway PSI
24 EvNO00084278 Plasmid Details pVP13 Bacterial (E. coli) expression, adds N-terminal S-tag, 6xHis and MBP, adds TEV site on 5p end of insert; amp resistance; recombinational cloning; Gateway cloning into this vector will remove the Gateway ccdB death casette. PSI
25 EvNO00084279 Plasmid Details pVP16 Bacterial (E. coli) expression, adds N-terminal 8xHis and MBP, adds TEV site on 5p end of insert; amp resistance; recombinational cloning; Gateway cloning into this vector will remove the Gateway ccdB death casette. PSI
26 EvNO00084280 Plasmid Details pVP33A Bacterial (E. coli) expression, adds N-terminal 8xHis, MBP, 3CP cleavage, tetra-cys, Tev cleavage; amp resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte. PSI,Promega
27 EvNO00084281 Plasmid Details pVP56A Bacterial (E. coli) expression, adds N-terminal 8xHis, MBP, 3CP cleavage, Tev cleavage; amp resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte. PSI,Promega
28 EvNO00084282 Plasmid Details pVP56K Bacterial (E. coli) expression, adds N-terminal 8xHis, MBP, 3CP cleavage, Tev cleavage; kanamycin resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte. PSI,Promega
29 EvNO00084283 Plasmid Details pEU-His-FV Cell-free expression vector, adds N-terminal 6xHis tag; amp resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte. PSI,Promega
30 EvNO00084284 Plasmid Details pEU-GST-FV Cell-free expression vector, adds N-terminal GST tag and 3CP cleavage site; amp resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte. PSI,Promega
31 EvNO00084285 Plasmid Details pVP33K Bacterial (E. coli) expression, adds N-terminal 6xHis tag, MBP, 3CP cleavage, tetra-cys, Tev cleavage; kanamycin resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte. PSI,Promega
32 EvNO00085126 Plasmid Details p11 "Bacterial (E. coli) expression vector, adds His tag, TEV cleavage site; amp resistance; restriction enzyme cloning." PSI
33 EvNO00085127 Plasmid Details p15Tv-LIC Bacterial (E. coli) expression vector, adds His tag, TEV cleavage site; ampicillin resistance; recombinational cloning (InFusion, BseRI). PSI
34 EvNO00085128 Plasmid Details pDB.GST Bacterial expression vector that adds GST and TEV protease site; kanamycin resistance; restriction enzyme cloning. PSI
35 EvNO00085129 Plasmid Details pDB.HBP Bacterial expression vector that adds HBP and TEV protease site; kanamycin resistance; restriction enzyme cloning. PSI
36 EvNO00085130 Plasmid Details pDB.His.MBP "Bacterial expression vector that adds 6xHis tag, MBP, TEV protease site; kanamycin resistance; restriction enzyme cloning." PSI
37 EvNO00085131 Plasmid Details pDB.His.NusA Bacterial expression vector that adds N-terminal 6xHis tag, NusA, Tev protease site; kanamycin resistance; restriction enzyme cloning. PSI
38 EvNO00085132 Plasmid Details pDB.His.TRX "Bacterial expression vector that adds N-terminal 6xHis tag, thioredoxin and TEV protease sites; kanamycin resistance; restriction enzyme cloning." PSI
39 EvNO00085133 Plasmid Details pDB.TRX Bacterial expression vector that adds thioredoxin and TEV protease sites; kanamycin resistance; restriction enzyme cloning. PSI
40 EvNO00085134 Plasmid Details pDB-HisGST "Bacterial expression vector that adds N-terminal His6 tag, GST, TEV protease site; kanamycin resistance; restriction enzyme cloning." PSI
41 EvNO00085135 Plasmid Details pHM6g "Vector that adds N-terminal 6xHis tag, MBP, Tev protease site; kanamycin resistance; restriction enzyme cloning." PSI
42 EvNO00085136 Plasmid Details pLIC.HM6g "Bacterial expression vector, adds N-terminal 6xHis tag, MBP, Tev protease site; amp resistance; ligation independent cloning SmaI (LIC-SmaI)." PSI
43 EvNO00085137 Plasmid Details pLICB.H Bacterial expression vector that adds N-terminal 6xHis tag; amp resistance; ligation independent cloning SmaI (LIC-SmaI). PSI
44 EvNO00085138 Plasmid Details pLICB.HM "Bacterial expression vector that adds N-terminal 6xHis tag, MBP, TEV protease site; amp resistance; ligation independent cloning SmaI (LIC-SmaI)." PSI
45 EvNO00085139 Plasmid Details pLICB.HV "Bacterial expression vector that adds N-terminal 6xHis tag, TEV protease site; amp resistance; ligation independent cloning SmaI (LIC-SmaI)." PSI
46 EvNO00091443 Plasmid Details pNYCOMPS-LIC-FH10T+ (N term) Bacterial expression vector; T7 promoter; N terminal Flag epitope and 10X His tags; tags can be removed by TEV protease; kanamycin resistance; ligation independent cloning. PSI
47 EvNO00285791 Plasmid Details pSpeedET Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHHENLYFQG tag; kanamycin resistance; PIPE cloning. PSI
48 EvNO00285801 Plasmid Details pBY011 Yeast expression vector with ARS1, CEN4, Gal1-10 promoter; amp resistance (URA3 for yeast); recombinational cloning.
49 EvNO00287601 Plasmid Details pMCSG29 Bacterial expression vector with T7 promoter, C-terminal 10xHis tag, TEV site, C-terminal MBP with TVMV site; ampicillin resistance in bacterial; ligation independent cloning (LIC) PSI
50 EvNO00292960 Plasmid Details pH3c-LIC Bacterial expression vector with N-terminal 8x His tag separated from the insert by a 3C protease cleavage site; kanamycin resistance in bacteria; ligation independent cloning (LIC) PSI
51 EvNO00292961 Plasmid Details pLIC-CH Bacterial expression vector with C-terminal 8x His tag separated from the insert by a TEV protease cleavage site; kanamycin resistance in bacteria; ligation independent cloning (LIC) PSI
52 EvNO00292962 Plasmid Details pHM3C-LIC Bacterial expression vector with N-terminal 6xHis and MBP tags separated from the insert by a 3C protease cleavage site; kanamycin resistance in bacteria; ligation independent cloning (LIC) PSI
53 EvNO00304413 Plasmid Details pHIS8 Gateway compatible bacterial expression vector with T7 promoter, N- and C-terminal His tags and thrombin cleavage site; contains ccdB death cassette that is removed after recombinational cloning; kanamycin resistance in bacteria; recombinational cloning. PSI
54 EvNO00304414 Plasmid Details pHIS9 Gateway compatible bacterial expression vector with T7 promoter and N-terminal His tag; contains ccdB death cassette that is removed after recombinational cloning; kanamycin resistance in bacteria; recombinational cloning. PSI
55 EvNO00304415 Plasmid Details pMIS2.1mv Gateway compatible bacterial expression destination vector with N-terminal 8xHis tag and Mistic (from B. subtilis) tag; contains ccdB death cassette that is removed after recombinational cloning; ampicillin resistance in bacteria; recombinational cloning. PSI
56 EvNO00304416 Plasmid Details pSGP46 Yeast expression vector with Adh2 promoter, adds C-terminal 3C-ZZ-10XHis tag; ampicillin resistance (bacteria); uracil resistance (yeast); ligation independent cloning (LIC). PSI
57 EvNO00304417 Plasmid Details pSGP47 Yeast expression vector with Adh2 promoter, adds 3C protease cleavage site and 10xHis tag; ampicillin resistance (bacteria); uracil (yeast); ligation independent cloning (LIC). PSI
58 EvNO00304418 Plasmid Details pSGP36 Yeast expression vector with Gal1 promoter, adds C-terminal 3C-his10 tag; amp resistance (URA for yeast); ligation independent cloning (LIC). PSI
59 EvNO00304419 Plasmid Details pSGP40 Yeast expression vector with Gal1 promoter, adds C-terminal 3C-ZZ-His10 tag; amp resistance (URA for yeast); ligation independent cloning (LIC). PSI
60 EvNO00304420 Plasmid Details pNYCOMPS-TH-ccdB (C-term.) Bacterial (e. coli) expression vector with T7 promoter, C-terminal 10xHis tag and TEV protease cleavage site; contains ccdB death cassette which is removed after LIC cloning; kanamycin resistance in bacteria; ligation independent cloning. PSI
61 EvNO00305686 Plasmid Details pMA507 E. coli expression vector with a T7 promoter, N-terminal 6xHis tag and TEV protease cleavage site; contains the ccdB death cassette; kanamycin resistance in bacteria; ligation independent cloning (LIC). PSI
62 EvNO00305687 Plasmid Details pMA510 Bacterial expression vector with a T7 promoter, N-terminal MBP fusion-6xHis tag-TEV protease site; contains the ccdB death cassette; kanamycin resistance in bacteria; ligation independent cloning (LIC). PSI
63 EvNO00305688 Plasmid Details pMAPLe003 Bacterial expression vector with a T7 promoter, N-terminal MBP and C-terminal 6xHis tag-TEV protease site; constitutively expresses TEV protease to produce C-terminal His-tagged protein; contains the ccdB death cassette; kanamycin resistance in bacteria; ligation independent cloning (LIC). PSI
64 EvNO00306565 Plasmid Details pMYC4-H Mycoplasma coexpression expression vector with the Phsp promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning ( NdeI, SbfI). PSI
65 EvNO00306566 Plasmid Details pMYC5-H Mycoplasma coexpression expression vector with the Phsp promoter and C-termial c-myc tag; modified from pMYC4-H with unique SrfI site; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (NdeI, SbfI). PSI
66 EvNO00306567 Plasmid Details pMYC4-I Mycoplasma coexpression expression vector with the Pimyc promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PacI, SbfI). PSI
67 EvNO00306568 Plasmid Details pMYC4-S Mycoplasma coexpression expression vector with the Psmyc promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PacI, SbfI). PSI
68 EvNO00306569 Plasmid Details pMYC4-Hp Mycoplasma coexpression expression vector with the Phsp promoter and C-termial c-myc tag; newer version of pMYC4-H suitable for universal cloning by PacI and SbfI sites; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PacI, SbfI). PSI
69 EvNO00306570 Plasmid Details p4-Hb Mycoplasma coexpression expression vector with the Phsp promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (AsiSI, PmeI). PSI
70 EvNO00306571 Plasmid Details p4-Ib Mycoplasma coexpression expression vector with the Pimyc promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (AsiSI, PmeI). PSI
71 EvNO00306572 Plasmid Details p4-Sb Mycoplasma coexpression expression vector with the Psmyc promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (AsiSI, PmeI). PSI
72 EvNO00306573 Plasmid Details p9T-c Mycoplasma coexpression expression vector with the PtetO promoter; tetracycline-inducible expression of insert; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PtetO gene: AsiSI, PmeI; second constitutively expressed gene: PacI, SbfI). PSI
73 EvNO00306585 Plasmid Details p9T-H Mycoplasma coexpression expression vector with the PtetO promoter and Phsp promoter; tetracycline-inducible expression of insert; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PtetO gene: AsiSI, PmeI; Phsp gene: PacI, SbfI). PSI
74 EvNO00306605 Plasmid Details p9T-I Mycoplasma coexpression expression vector with the PtetO promoter and Pimyc promoter; tetracycline-inducible expression of insert; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PtetO gene: AsiSI, PmeI; Pimyc gene: PacI, SbfI). PSI
75 EvNO00306625 Plasmid Details p9T-S Mycoplasma coexpression expression vector with the PtetO promoter and Psmyc promoter; tetracycline-inducible expression of insert; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PtetO gene: AsiSI, PmeI; Psmyc gene: PacI, SbfI). PSI
76 EvNO00306645 Plasmid Details pVP80K Bacterial expression vector with a T5 promoter and N-terminal 8xHis-MBP tag; kanamycin resistance in bacteria; restriction enzyme cloning. PSI
77 EvNO00306665 Plasmid Details pVP81K Bacterial expression vector with a T5 promoter with an auto-cleaving (TVMV protease) N-terminal MBP tag that leaves an N-terminal 8xHis tagged protein; kanamycin resistance in bacteria; restriction enzyme cloning. PSI
78 EvNO00306666 Plasmid Details pVP91A Bacterial expression vector with a T5 promoter and N-terminal 8xHis tag; ampicilliin resistance in bacteria; restriction enzyme cloning. PSI
79 EvNO00306667 Plasmid Details pEU-HSBC In Vitro SP6 transcription and wheat germ cell free protein expression vector with a SP6 promoter and N-terminal 6xHis tag; ampicillin resistance in bacteria; restriction enzyme cloning. PSI
80 EvNO00306668 Plasmid Details pVP65K Bacterial (E. coli) expression with N-terminal 8xHis, MBP tag; kanamycin resistance in bacteria; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat cassette. PSI,Promega
81 EvNO00306669 Plasmid Details pVP68K Bacterial (E. coli) expression with N-terminal 8xHis, MBP tag; kanamycin resistance in bacteria; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat cassette. PSI,Promega
82 EvNO00335423 Plasmid Details ZM552 Yeast expression vector with N-terminal 3X V5 epitope tag; ampicillin resistance in bacteria; LEU2 resistance in yeast; Gateway cloning.
83 EvNO00336943 Plasmid Details pET15_NESG Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning. PSI/NESG
84 EvNO00336944 Plasmid Details pET21_NESG Bacterial (E. coli) expression vector with T7 lac promoter, adds C-terminal 6xHis tag; ampicillin resistance; restriction enzyme cloning. PSI/NESG
85 EvNO00340963 Plasmid Details pMCSG26 Bacterial expression vector with C-terminal 6xHis tag and T7 promoter; ampicillin resistance in bacterial; ligation independent cloning (LIC) PSI
86 EvNO00340964 Plasmid Details pMCSG28 Bacterial expression vector with T7 promoter and TEV-cleavage C-terminal 6xHis; ampicillin resistance in bacterial; ligation independent cloning (LIC) PSI
87 EvNO00340965 Plasmid Details pMCSG32 Bacterial expression vector with T7 promoter and C-terminal 6xHis, TEV-protease cleavage site, N-terminal MBP tag with TVMV site; ampicillin resistance in bacterial; ligation independent cloning (LIC) PSI
88 EvNO00412303 Plasmid Details pCPD_nGST-SBP_DC Bacterial expression vector with a T7 promoter, N-terminal GST, Thrombin prtease cleavage site, SBP tag, and TEV protease cleavage site; ampicillin and chloamphenicol resistance in bacteria (CmR removed in clones with inserts); Gateway recombinational cloning.
89 EvNO00412304 Plasmid Details pCPD_nMBP-SBP_DC with tag/fusion/marker assay, Gateway&reg, acceptor (destination), recombinational cloning clone, bacterial expression expression with pCPD5698_F and pCPD6268_R and MBP and Thrombin and SBP and TEV and ccdB and CmR and T7 term and LacI and AmpR and ColE and T7 and lac operator and RBS; chloramphenicol resistance in bacterial, ampicillin resistance in bacterial;
90 EvNO00413803 Plasmid Details pCPD_nSBP-Halo_DC Bacterial expression vector with a T7 promoter and N-terminal SBP and Halo tags, seperated by protease cleavage sites for thrombin and TEV protease; ampicillin resistance in bacteria; Gateway cloning HALO
91 EvNO00413804 Plasmid Details pCPD_nHalo_DC Bacterial expression vector with a T7 promoter and TEV cleavable N-terminal Halo tag; ampicillin resistance in bacteria; Gateway cloning HALO
92 EvNO00424503 Plasmid Details pJFT7_nHALO_DC In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable HALO tag; ampicillin resistance; Gateway or Flexi cloning compatible HALO
93 EvNO00424523 Plasmid Details pJFT7_cHalo_DC In vitro expression vector with T7 promoter and C-terminal TEV-cleavable Halo tag; ampicillin resistance; Gateway or Flexi cloning compatible HALO
94 EvNO00424543 Plasmid Details pJFT7_nHalo_empty In vitro expression vector with T7 promoter and N-terminal TEV-cleavable Halo tag; does not contain a death cassette; ampicillin resistance; restriction enzyme cloning HALO
95 EvNO00424544 Plasmid Details pJFT7_cHalo_empty In vitro expression vector with T7 promoter and C-terminal TEV-cleavable Halo tag; does not contain a death cassette; ampicillin resistance; restriction enzyme cloning HALO
96 EvNO00430383 Plasmid Details pSGP17 Yeast expression vector with a AOX1 promoter C-terminal CBP and RSG-6xHis tag with 3C protease cleavage site and pre-pro-alpha-factor leader sequence; zeocin resistance in Pichia and in bacteria; ligation independent cloning (LIC) PSI
97 EvNO00430384 Plasmid Details pSGP18 Yeast expression vector with a AOX1 promoter C-terminal CBP and RSG-6xHis tag with 3C protease cleavage site; zeocin resistance in Pichia and in bacteria; ligation independent cloning (LIC) PSI
98 EvNO00450863 Plasmid Details pMCSG53 Bacterial expression vector with a T7 promoter and TEV-cleavable N-terminal 6xHis tag; Contains rare argU and ileX tRNA genes; ampicillin resistance in bacteria; ligation independent cloning. PSI
99 EvNO00450864 Plasmid Details pMCSG68 Bacterial expression vector with a T7 promoter and TEV-cleavable N-terminal 6xHis and StrepII tags; rare argU and ileX codons; ampicillin resistance in bacteria; ligation independent cloning PSI
100 EvNO00450865 Plasmid Details pMCSG69 Bacterial expression vector with a T7 promoter and N-terminal MBP tag, TVMV cleavage site, 6xHis tag and TEV protease cleavage site with rare codon argU and ileX tRNA genes; ampicillin resistance in bacteria; ligation independent cloning PSI
101 EvNO00450883 Plasmid Details pJSP6_nHalo_DC Wheat germ expression vector with a SP6 promoter and N-terminal TEV-cleavable HALO tag; ampicillin resistance in bacteria and chloramphenicol resistance in empty vector; Gateway or Flexi cloning HALO
102 EvNO00450884 Plasmid Details pJSP6_nHalo_empty Wheat germ expression vector with SP6 promoter and TEV-cleavable N-terminal HALO tag; ampicillin resistance in bacteria; gateway cloning HALO
103 EvNO00454843 Plasmid Details pMCSG49 Bacterial expression vector with a T7 promoter and N-terminal 6xHis tag, TEV cleavable; ampicillin resistance in bacteria; Ligation Independent cloning PSI
104 EvNO00454845 Plasmid Details pMCSG54 Bacterial expression vector with a T7 promoter and N-terminal Flag and 6xHiis tag followed by TEV protease cleavage site ; ampicillin resistance in bacteria; ligation independent cloning. PSI
105 EvNO00454846 Plasmid Details pMCSG55 Bacterial expression vector with T7 promoter and N-terminal 6xHis and HA tags followed by TEV protease cleavage site; ampicillin resistance in bacteria; ligation independent cloning PSI
106 EvNO00454847 Plasmid Details pMCSG56 Bacterial expression vector with T7 promoter and N-terminal 6xHis and double HA tags followed by TEV protease cleavage site; ampicillin resistance in bacteria; ligation independent cloning PSI
107 EvNO00534623 Plasmid Details pGEn1-DEST Mammalian expression vector with a CMV promoter and N-terminal TCM 8xHis and Strep tag; ampicillin resistance in bacteria; Gateway recombinational cloning SPTA
108 EvNO00534624 Plasmid Details pGEn2-DEST Mammalian expression vector with a CMV promoter and N-terminal 8xHis, AviTag, and Super GFP tags; ampicillin resistance in bacteria; Gateway recombinational cloning SPTA
109 EvNO00534626 Plasmid Details pGEc1-DEST Mammalian expression vector with a CMV promoter and C-terminal 8xHis and Strep tags; ampicillin resistance in bacteria; Gateway recombinational cloning SPTA
110 EvNO00534627 Plasmid Details pGEc2-DEST Mammalian expression vector with a CMV promoter and C-terminal Superfolder GFP, AviTag and 10xHis tag; ampicillin resistance in bacteria; Gateway recombinational cloning SPTA
111 EvNO00548203 Plasmid Details pET16-DEST Bacterial expression vector with a T7 promoter and a N-terminal 10xHis tag; ampicillin resistance in bacteria; Gateway recombinational cloning. SPTA
112 EvNO00548204 Plasmid Details pET32-DEST Bacterial expression vector with T7 promoter and N-terminal Trx, 6xHis and S-tags; Thrombin and enterokinase cleavage sites; ampicillin resistance in bacteria; Gateway recombinational cloning SPTA
113 EvNO00587843 Plasmid Details pJSP6_nGST_DC Wheat germ expression vector with a SP6 promoter and N-terminal TEV-cleavable GST tag; ampicillin resistance; Gateway or Flexi cloning compatible
114 EvNO00595403 Plasmid Details pJFT7_nHIS_cHalo_DC In vitro expression vector with a T7 promoter and N-terminal HIS and C-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible HALO
115 EvNO00595404 Plasmid Details pJFT7_cHalo-3xHA_DC In vitro expression vector with a T7 promoter and C-terminal TEV-cleavable HALO-3xHA tag; ampicillin resistance; Gateway or Flexi cloning compatible HALO
116 EvNO00595405 Plasmid Details pJFT7_nFlag_cHalo_DC In vitro expression vector with a T7 promoter and N-terminal Flag and C-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible HALO
117 EvNO00595406 Plasmid Details pJFT7_nHA_cHalo_DC In vitro expression vector with a T7 promoter and N-terminal HA and C-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible HALO
118 EvNO00595407 Plasmid Details pJFT7_n3xFlag-Halo_DC In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable 3xFlag-Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible HALO
119 EvNO00595408 Plasmid Details pJFT7_cHalo-3xFlag_DC In vitro expression vector with a T7 promoter and C-terminal TEV-cleavable HALO-3xFlag tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible HALO
120 EvNO00595409 Plasmid Details pJFT7_n3xHA-Halo_DC In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable 3xHA-Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible HALO
121 EvNO00599463 Plasmid Details pMCSG58 Bacterial expression vector with T7 promoter and C-terminal 6xHis tag; contain argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning PSI
122 EvNO00599464 Plasmid Details pMCSG59 Bacterial expression vector with T7 promoter and TEV-cleavable C-terminal 6xHis tag; contain argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning PSI
123 EvNO00599484 Plasmid Details pMCSG63 Bacterial co-expresion vector with T7 promoter and N-terminal TEV-cleavable 6xHis tag; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning PSI
124 EvNO00599485 Plasmid Details pMCSG70 Bacterial expression vector with T7 promoter and C-terminal TEV-cleavable 10xHis tag and TVMV-cleavable MBP tag; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning PSI
125 EvNO00599486 Plasmid Details pMCSG71 Bacterial expression vector with T7 promoter, N-terminal TVMV-cleavable MBP tag and N-terminal TEV-cleavable 6xHis tag; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning PSI
126 EvNO00599503 Plasmid Details pMCSG75 Bacterial expression vector with T7 promoter and TEV-cleavable 6xHis tag BirA gene; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning PSI
127 EvNO00599504 Plasmid Details pMCSG76 Bacterial co-expression vector with T7 promoter and N-terminal TEV-cleavable 6xHis tag; contains argU and ileX rare tRNA; spectinomycin resistance in bacteria; ligation independent cloning PSI
128 EvNO00599523 Plasmid Details pMCSG77 Bacterial co-expression vector with T7 promoter and N-terminal TEV-cleavable 6xHis tag; contains argU and ileX rare tRNA; kanamycin resistance in bacteria; ligation independent cloning PSI
129 EvNO00601503 Plasmid Details pLIFE-3'UTR Mammalian expression vector for detecting the expression of 3'UTRs by Firefly luciferase; also contains Renilla luciferase for normalization; ampicillin resistance in bacteria; Gateway cloning with P2RP3 Gateway Cassette SPTA
130 EvNO00601504 Plasmid Details pLIFE-miRNA Mammalian expression vector for expressing miRNAs; also contains red fluorescent protein, gateway cassette located within an intron of RFP 3'UTR, for ncRNA expression; ampicillin resistance in bacteria; Gateway cloning with L2R3 Gateway Cassette SPTA
131 EvNO00601603 Plasmid Details pCMV(delta1) Mammalian expression vector with a deletion mutant of the CMV promoter; ampicillin resistance in bacteria, neomycin resistance in mammalian cells; restriction enzyme cloning SPTA
132 EvNO00601604 Plasmid Details pCMV(delta2) Mammalian expression vector with a deletion mutant of the CMV promoter; ampicillin resistance in bacteria, neomycin resistance in mammalian cells; restriction enzyme cloning SPTA
133 EvNO00601605 Plasmid Details pCMV(delta3) Mammalian expression vector with a deletion mutant of the CMV promoter; ampicillin resistance in bacteria, neomycin resistance in mammalian cells; restriction enzyme cloning SPTA
134 EvNO00601606 Plasmid Details pCMV(delta4) Mammalian expression vector with a deletion mutant of the CMV promoter; ampicillin resistance in bacteria, neomycin resistance in mammalian cells; restriction enzyme cloning SPTA
135 EvNO00601607 Plasmid Details pCMV(delta5) Mammalian expression vector with a deletion mutant of the CMV promoter; ampicillin resistance in bacteria, neomycin resistance in mammalian cells; restriction enzyme cloning SPTA
136 EvNO00601608 Plasmid Details pCMV(delta6) Mammalian expression vector with a deletion mutant of the CMV promoter; ampicillin resistance in bacteria, neomycin resistance in mammalian cells; restriction enzyme cloning SPTA
137 EvNO00601609 Plasmid Details pCMV(WT) Mammalian expression vector with a CMV promoter; ampicillin resistance in bacteria, neomycin resisatance in mammalian cells; restriction enzyme cloning SPTA
138 EvNO00621023 Plasmid Details pCPD_nHalo_cHIS_DC Bacterial expression with a T7 promoter, N-terminal TEV-cleavable HaloTag and C-terminal 3C protease cleavable 6xHis tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible HALO
139 EvNO00621024 Plasmid Details pJFT7_nHalo_DC(r3) In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible HALO
140 EvNO00623103 Plasmid Details pJFT7_nGST_DC-r2 In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
141 EvNO00623703 Plasmid Details pRSET-natGFP Bacterial expression vector with a T7 promoter; adds C-terminal GFP tag; ampicillin resistance in bacteria; ligation independent cloning PSI
142 EvNO00623704 Plasmid Details pRSET-natGFPHis Bacterial expression vector with a T7 promoter; adds C-terminal GFP-His6 tag; ampicillin resistance in bacteria; ligation independent cloning PSI
143 EvNO00623723 Plasmid Details pJFT7_nHalo_DC(r4) In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible HALO
144 EvNO00623724 Plasmid Details pJSP6_cHalo_DC Wheat germ expression vector with SP6 promoter and TEV-cleavable C-terminal HALO tag; ampicillin resistance in bacteria; gateway cloning HALO
145 EvNO00629003 Plasmid Details pET-32b-TEV Bacterial expression vector with T7 promoter and N-terminal E. coli thioredoxin tag, 6xHis-tag, thrombin cleavage site, S-tag, and TEV cleavage site; ampicillin resistance in bacteria; restriction enzyme cloning PSI
146 EvNO00629004 Plasmid Details pRSET-natTEVGFPHis Bacterial expression vector with T7 promoter and C-terminal TEV-cleavavable GFP and 6xHis-tags; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI PSI
147 EvNO00629005 Plasmid Details pCMVi-LSrCOMPTT with tag/fusion/marker assay, ligation independent cloning (LIC) clone, mammalian expression expression with intron and CMV and oligomerization domain and hGH and SV40 and leader sequence and AmpR and f1 and ColE and peptide and -10/Spec-R and COMP and Tetanus Toxoid and epitope and Stop Codons and InTron For and 013_DO3_DTH21 and RBS and 014_C03_DTH22 and 2012 and HGHTER and CMV-F; ampicillin resistance in bacterial; PSI
148 EvNO00629006 Plasmid Details pCMVi-UB Vector for antibody production in mice upon genetic immunization by gene gun with CMV promoter and N-terminal mouse ubiqiutin protein with Gly76Ala mutation; ampicillin resistance in bacteria; restriction enzyme cloning PSI
149 EvNO00629007 Plasmid Details pBAD24sigNtermHis Arabinose-inducible bacterial expression with a pBAD promoter and N-terminal E. coli YtfM (aa1-21) and 6xHis-tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI PSI
150 EvNO00629008 Plasmid Details pBAD24sigCtermHis Arabinose-inducible expression in bacteria with N-terminal E. coli YtfM (aa1-21) signal sequence and C-terminal His-tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI PSI
151 EvNO00629009 Plasmid Details pRSET-YtfM-8xHis-N-term Bacterial expression with T7 promoter and N-terminal E. coli YtfM (aa1-21) signal sequence and 8xHis-tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI PSI
152 EvNO00629010 Plasmid Details pRSET-C8xHis Bacterial expression vector with T7 promoter and C-terminal 8xHis tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI PSI
153 EvNO00629011 Plasmid Details pRSET-C6xHis Bacterial expression vector with T7 promoter and C-terminal 6xHis tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI PSI
154 EvNO00631283 Plasmid Details pCMVi-LS with tag/fusion/marker assay, multiple cloning site clone, bacterial expression expression with leader sequence and MCS and intron and hGH and forward primer and AmpR and ColE and f1 and reverse primer and DTH and -10/Spec-R and Stop Codons and CMV and SV40; ampicillin resistance in bacterial; PSI
155 EvNO00631284 Plasmid Details pRSET-YtfM-AN Bacterial expression vector with T7 promoter and N-terminal E. coli YtfM (aa1-23) and 7xHis-tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI PSI
156 EvNO00631285 Plasmid Details pCDF-BAD Arabinose-inducible expression in bacteria; spectinomycin resistance in bacteria; ligation into multiple cloning site downstream of RBS: NdeI, XhoI/SciI, AscI, ZraI/AatII; or just upstream of RBS: EagI/NotI, PmeI PSI
157 EvNO00665463 Plasmid Details pANT7_GST-control In vitro expression vector expressing two GSTs as a control vector for clones in pANT7_cGST; ampicillin resistance in bacteria; recombinational cloning.
158 EvNO00813763 Plasmid Details pPPL ligation independent cloning (LIC) clone with pBR322 origin and Ampicillin Resistance and ROP and ORF frame 2 Tetracycline resistance and ORF frame 1 and pBRrevBam and T7 and T7 term primer and BseRI Stuffer and MBP signal sequence (M1-A26 of NP_290668) and RBS and MBP (K27-T392 of NP_290668) and HIS10 and TEV and FC sequencing primer pET26 pelB seq R and FC sequencing primer MBP pelB seqF and FC sequencing primer pET-PPL seq 1 F and FC sequencing primer pET-PPL seq 1 R and FC sequencing primer pET-PPL seq 2 F and T7 term and FC sequencing primer pET-PPL seq 2 R and AmpR; ampicillin resistance in bacterial;
159 EvNO00813764 Plasmid Details pET-BAD ligation independent cloning (LIC) clone, bacterial expression expression with Operator O2 and araC promoter and T7 term and Operator O1 and B-lactamase gene and CAP site and T7 term primer and pBR322 origin and Operator I1 and I2 and arabinose BAD promoter and rrnB T1 T2 and RBS and ARAC3F Primer and PCBSREV Primer and ORICOL Primer and BADT1A Primer and BADT1B Primer and AMP5R Primer and -35 AP and F1ORI Primer and ARAC5R and -10 AP and rep_origin and ROP and araC; ampicillin resistance in bacterial;
160 EvNO00813765 Plasmid Details pRSF-BAD ligation independent cloning (LIC) clone, bacterial expression expression with Kan-R and T7TER primer (#35) and T7 Terminator and RNA II and RNA I and RSF ori - RSF1030 Homology and -10 RNA I and -35 RNA I and -35 AP and -10 AP and RBS-reverse-weak and araC and Operator O2 and araC promoter and Operator O1 and CAP site and Operator I1 and I2 and arabinose BAD promoter and rrnB T1 T2 and RBS and ligation junction Xbal and BADT1A Primer and BADT1B Primer and ARAC3F primer (#145) and ARAC5R primer (#148) and wrong primer #62 off by 3 nt and KAN1F primer (#120) and ligation junction Pacl; 50ug/mL of Kanamycin resistance in bacterial;
161 EvNO00813783 Plasmid Details pelB-MBP ligation independent cloning (LIC) clone, bacterial expression expression with RBS (extended) and TET-2 and pelB and pBR322 origin and KanR and ROP and BseRI and HIS10 and MBP and TEV and FC sequencing primer pET26 pelB seq R and FC sequencing primer MBP pelB seqF and TET and LacI and FC sequencing primer pET-PPL seq 1 F and FC sequencing primer pET-PPL seq 1 R and FC sequencing primer pET-PPL seq 2 F and pBRrevBam and T7 and lac operator and T7 term primer; kanamycin resistance in bacterial;
162 EvNO00813803 Plasmid Details pCPD-pRL-CMV-ccdb fluorescent marker assay, Gateway cloning, bacterial expression expression with Renilla Luciferase and pCEP Fwd Primer and ccdB and CMV and CAGF and CMV-F and Amp-R and lac promoter and attR1 and attR2 and T7 promoter and Chloramphenicol-R; chloramphenicol resistance in bacterial, ampicillin resistance in bacterial;
163 EvNO00846203 Plasmid Details pPSU1 High copy number plasmid which provides 500, 1000, 1500, 2000 and 5000 bp EcoRV fragments of kilobase DNA ladder, and 500, 700, 800, 900, 1000, 2000 and 4100 bp PstI fragments of 100 bp DNA ladder. Confers ampicillin resistance.
164 EvNO00846223 Plasmid Details pPSU2 High copy number plasmid which provides 750, 3000 and 4000 bp EcoRV fragments of kilobase DNA ladder, and 50, 100, 200, 300, 400, 500, 600, 1500 and 4100 bp PstI fragments of 100 bp DNA ladder. Confers ampicillin resistance.
165 EvNO00877063 Plasmid Details pLX304 with tag/fusion/marker assay, Gateway&reg, acceptor (destination), recombinational cloning clone, lentiviral infection expression with BlastR and T7 and attR1 and CMV and cPPT and V5 and AmpR and attR2 and CAT and ccdB and WPRE; ampicillin resistance in bacterial, blasticidin resistance in mammalian;
166 EvNO00877064 Plasmid Details pLV_R4R2_GTW3 with tag/fusion/marker assay, Gateway&reg clone, retroviral vector expression with RRE; chloramphenicol resistance in bacterial, ampicillin resistance in bacterial, blasticidin resistance in mammalian;
167 EvNO00877066 Plasmid Details pMK-Dest with tag/fusion/marker assay, Gateway&reg clone, bacterial expression expression with CMV and CMV-F and attR1 and attR2 and lac promoter and pBR322 origin and NeoR and AttB2 and TEV; kanamycin resistance in bacterial, neomycin resistance in bacterial;

  Clone ID Vector Name Description Special
MTA
 
1 EvNO00084258 Plasmid Details pMCSG10 with tag/fusion/marker assay, multiple cloning site clone, bacterial expression, improves solubility of insert expression with lacI and LIC and AmpR and T7 and T7 term and ori and TEV and His and His 2 and GST; ampicillin resistance in bacterial; PSI
2 EvNO00084259 Plasmid Details pMCSG11 Coexpression vector with T7 promoter, adds N-terminal His tag, TEV, SNA; chloramphenicol resistance; ligation independent cloning (LIC). PSI
3 EvNO00084260 Plasmid Details pMCSG12 Coexpression vector with T7 promoter, adds N-terminal His tag, Sloop, TEV, SNA; chloramphenicol resistance; ligation independent cloning (LIC). PSI
4 EvNO00084261 Plasmid Details pMCSG13 Coexpression vector with T7 promoter, adds N-terminal His tag, MBP, TEV, SNA; chloramphenicol resistance; ligation independent cloning (LIC). PSI
5 EvNO00084262 Plasmid Details pMCSG14 Coexpression vector with T7 promoter, adds N-terminal His tag, GST, TEV, SNA; chloramphenicol resistance; ligation independent cloning (LIC). PSI
6 EvNO00084263 Plasmid Details pMCSG17 Coexpression vector with T7 promoter, adds N-terminal Stag, TEV, SNA; amp resistance; ligation independent cloning (LIC). PSI
7 EvNO00084264 Plasmid Details pMCSG18 Screening vector with T7 promoter, adds N-terminal His tag, TEV, LICs, GFP; amp resistance; ligation independent cloning (LIC). PSI
8 EvNO00084265 Plasmid Details pMCSG19 Vector for solubility of insert and for purification, with T7 promoter, adds N-terminal MBP, TVMV, His, TEV, NA; amp resistance; ligation independent cloning (LIC). PSI
9 EvNO00084266 Plasmid Details pMCSG20 Coexpression vector with T7 promoter, adds N-terminal Stag, GST, TEV, SNA; amp resistance; ligation independent cloning (LIC PSI
10 EvNO00084267 Plasmid Details pMCSG21 Coexpression vector with T7 promoter, adds N-terminal His, TEV, SNA; spectinomycin resistance; ligation independent cloning (LIC). PSI
11 EvNO00084268 Plasmid Details pMCSG22 Coexpression vector with T7 promoter, adds N-terminal His, Sloop, TEV, SNA; spectinomycin resistance; ligation independent cloning (LIC). PSI
12 EvNO00084269 Plasmid Details pMCSG23 Coexpression vector with T7 promoter, adds N-terminal His, MBP, TEV, SNA; spectinomycin resistance; ligation independent cloning (LIC). PSI
13 EvNO00084270 Plasmid Details pMCSG24 Coexpression vector with T7 promoter, adds N-terminal His, GST, TEV, SNA; spectinomycin resistance; ligation independent cloning (LIC). PSI
14 EvNO00084271 Plasmid Details pMCSG7 with tag/fusion/marker assay, ligation independent cloning SspI (LIC-SspI), bacterial expression, in vitro transcription expression with AmpR and His and LIC and ori and T7 and T7 term and TEV and His 2 and LacI and lac operator; ampicillin resistance in bacterial; PSI
15 EvNO00084272 Plasmid Details pMCSG8 Vector facillitating reduced protein toxicity, with T7 promoter, adds N-terminal His tag, Sloop, TEV, SNA; amp resistance; ligation independent cloning (LIC). PSI
16 EvNO00084273 Plasmid Details pMCSG9 Vector with tags for solubility of insert, with T7 promoter, adds N-terminal His tag, MBP, TEV, SNA; amp resistance; ligation independent cloning (LIC). PSI
17 EvNO00084274 Plasmid Details pMCSG19C Vector for solubility of insert and for purification, with T7 promoter, adds N-terminal MBP, TVMV, His, TEV, NA; amp resistance; ligation independent cloning (LIC). PSI
18 EvNO00084275 Plasmid Details pMCentr1 cloning vector to link to Gateway system, with attL1-TEV-LIC site-attL2; zeocin resistance; ligation independent cloning to make pENTR vector for recombinational cloning PSI
19 EvNO00084276 Plasmid Details pMCentr2 cloning vector to link to Gateway system, with attL1-TEV-LIC site-attL2; zeocin resistance; ligation independent cloning to make pENTR vector for recombinational cloning PSI
20 EvNO00084277 Plasmid Details pMCentr3 destination vector to accept inserts from pENTR clones; attL1-TVMV-LIC site-attL2; kanamycin resistance; ligation independent cloning to Gateway PSI
21 EvNO00084278 Plasmid Details pVP13 Bacterial (E. coli) expression, adds N-terminal S-tag, 6xHis and MBP, adds TEV site on 5p end of insert; amp resistance; recombinational cloning; Gateway cloning into this vector will remove the Gateway ccdB death casette. PSI
22 EvNO00084279 Plasmid Details pVP16 Bacterial (E. coli) expression, adds N-terminal 8xHis and MBP, adds TEV site on 5p end of insert; amp resistance; recombinational cloning; Gateway cloning into this vector will remove the Gateway ccdB death casette. PSI
23 EvNO00084280 Plasmid Details pVP33A Bacterial (E. coli) expression, adds N-terminal 8xHis, MBP, 3CP cleavage, tetra-cys, Tev cleavage; amp resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte. PSI,Promega
24 EvNO00084281 Plasmid Details pVP56A Bacterial (E. coli) expression, adds N-terminal 8xHis, MBP, 3CP cleavage, Tev cleavage; amp resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte. PSI,Promega
25 EvNO00084282 Plasmid Details pVP56K Bacterial (E. coli) expression, adds N-terminal 8xHis, MBP, 3CP cleavage, Tev cleavage; kanamycin resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte. PSI,Promega
26 EvNO00084283 Plasmid Details pEU-His-FV Cell-free expression vector, adds N-terminal 6xHis tag; amp resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte. PSI,Promega
27 EvNO00084284 Plasmid Details pEU-GST-FV Cell-free expression vector, adds N-terminal GST tag and 3CP cleavage site; amp resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte. PSI,Promega
28 EvNO00084285 Plasmid Details pVP33K Bacterial (E. coli) expression, adds N-terminal 6xHis tag, MBP, 3CP cleavage, tetra-cys, Tev cleavage; kanamycin resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte. PSI,Promega
29 EvNO00085126 Plasmid Details p11 "Bacterial (E. coli) expression vector, adds His tag, TEV cleavage site; amp resistance; restriction enzyme cloning." PSI
30 EvNO00085127 Plasmid Details p15Tv-LIC Bacterial (E. coli) expression vector, adds His tag, TEV cleavage site; ampicillin resistance; recombinational cloning (InFusion, BseRI). PSI
31 EvNO00085128 Plasmid Details pDB.GST Bacterial expression vector that adds GST and TEV protease site; kanamycin resistance; restriction enzyme cloning. PSI
32 EvNO00085129 Plasmid Details pDB.HBP Bacterial expression vector that adds HBP and TEV protease site; kanamycin resistance; restriction enzyme cloning. PSI
33 EvNO00085130 Plasmid Details pDB.His.MBP "Bacterial expression vector that adds 6xHis tag, MBP, TEV protease site; kanamycin resistance; restriction enzyme cloning." PSI
34 EvNO00085131 Plasmid Details pDB.His.NusA Bacterial expression vector that adds N-terminal 6xHis tag, NusA, Tev protease site; kanamycin resistance; restriction enzyme cloning. PSI
35 EvNO00085132 Plasmid Details pDB.His.TRX "Bacterial expression vector that adds N-terminal 6xHis tag, thioredoxin and TEV protease sites; kanamycin resistance; restriction enzyme cloning." PSI
36 EvNO00085133 Plasmid Details pDB.TRX Bacterial expression vector that adds thioredoxin and TEV protease sites; kanamycin resistance; restriction enzyme cloning. PSI
37 EvNO00085134 Plasmid Details pDB-HisGST "Bacterial expression vector that adds N-terminal His6 tag, GST, TEV protease site; kanamycin resistance; restriction enzyme cloning." PSI
38 EvNO00085135 Plasmid Details pHM6g "Vector that adds N-terminal 6xHis tag, MBP, Tev protease site; kanamycin resistance; restriction enzyme cloning." PSI
39 EvNO00085136 Plasmid Details pLIC.HM6g "Bacterial expression vector, adds N-terminal 6xHis tag, MBP, Tev protease site; amp resistance; ligation independent cloning SmaI (LIC-SmaI)." PSI
40 EvNO00085137 Plasmid Details pLICB.H Bacterial expression vector that adds N-terminal 6xHis tag; amp resistance; ligation independent cloning SmaI (LIC-SmaI). PSI
41 EvNO00085138 Plasmid Details pLICB.HM "Bacterial expression vector that adds N-terminal 6xHis tag, MBP, TEV protease site; amp resistance; ligation independent cloning SmaI (LIC-SmaI)." PSI
42 EvNO00085139 Plasmid Details pLICB.HV "Bacterial expression vector that adds N-terminal 6xHis tag, TEV protease site; amp resistance; ligation independent cloning SmaI (LIC-SmaI)." PSI
43 EvNO00091443 Plasmid Details pNYCOMPS-LIC-FH10T+ (N term) Bacterial expression vector; T7 promoter; N terminal Flag epitope and 10X His tags; tags can be removed by TEV protease; kanamycin resistance; ligation independent cloning. PSI
44 EvNO00285791 Plasmid Details pSpeedET Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHHENLYFQG tag; kanamycin resistance; PIPE cloning. PSI
45 EvNO00287601 Plasmid Details pMCSG29 Bacterial expression vector with T7 promoter, C-terminal 10xHis tag, TEV site, C-terminal MBP with TVMV site; ampicillin resistance in bacterial; ligation independent cloning (LIC) PSI
46 EvNO00292960 Plasmid Details pH3c-LIC Bacterial expression vector with N-terminal 8x His tag separated from the insert by a 3C protease cleavage site; kanamycin resistance in bacteria; ligation independent cloning (LIC) PSI
47 EvNO00292961 Plasmid Details pLIC-CH Bacterial expression vector with C-terminal 8x His tag separated from the insert by a TEV protease cleavage site; kanamycin resistance in bacteria; ligation independent cloning (LIC) PSI
48 EvNO00292962 Plasmid Details pHM3C-LIC Bacterial expression vector with N-terminal 6xHis and MBP tags separated from the insert by a 3C protease cleavage site; kanamycin resistance in bacteria; ligation independent cloning (LIC) PSI
49 EvNO00304413 Plasmid Details pHIS8 Gateway compatible bacterial expression vector with T7 promoter, N- and C-terminal His tags and thrombin cleavage site; contains ccdB death cassette that is removed after recombinational cloning; kanamycin resistance in bacteria; recombinational cloning. PSI
50 EvNO00304414 Plasmid Details pHIS9 Gateway compatible bacterial expression vector with T7 promoter and N-terminal His tag; contains ccdB death cassette that is removed after recombinational cloning; kanamycin resistance in bacteria; recombinational cloning. PSI
51 EvNO00304415 Plasmid Details pMIS2.1mv Gateway compatible bacterial expression destination vector with N-terminal 8xHis tag and Mistic (from B. subtilis) tag; contains ccdB death cassette that is removed after recombinational cloning; ampicillin resistance in bacteria; recombinational cloning. PSI
52 EvNO00304416 Plasmid Details pSGP46 Yeast expression vector with Adh2 promoter, adds C-terminal 3C-ZZ-10XHis tag; ampicillin resistance (bacteria); uracil resistance (yeast); ligation independent cloning (LIC). PSI
53 EvNO00304417 Plasmid Details pSGP47 Yeast expression vector with Adh2 promoter, adds 3C protease cleavage site and 10xHis tag; ampicillin resistance (bacteria); uracil (yeast); ligation independent cloning (LIC). PSI
54 EvNO00304418 Plasmid Details pSGP36 Yeast expression vector with Gal1 promoter, adds C-terminal 3C-his10 tag; amp resistance (URA for yeast); ligation independent cloning (LIC). PSI
55 EvNO00304419 Plasmid Details pSGP40 Yeast expression vector with Gal1 promoter, adds C-terminal 3C-ZZ-His10 tag; amp resistance (URA for yeast); ligation independent cloning (LIC). PSI
56 EvNO00304420 Plasmid Details pNYCOMPS-TH-ccdB (C-term.) Bacterial (e. coli) expression vector with T7 promoter, C-terminal 10xHis tag and TEV protease cleavage site; contains ccdB death cassette which is removed after LIC cloning; kanamycin resistance in bacteria; ligation independent cloning. PSI
57 EvNO00305686 Plasmid Details pMA507 E. coli expression vector with a T7 promoter, N-terminal 6xHis tag and TEV protease cleavage site; contains the ccdB death cassette; kanamycin resistance in bacteria; ligation independent cloning (LIC). PSI
58 EvNO00305687 Plasmid Details pMA510 Bacterial expression vector with a T7 promoter, N-terminal MBP fusion-6xHis tag-TEV protease site; contains the ccdB death cassette; kanamycin resistance in bacteria; ligation independent cloning (LIC). PSI
59 EvNO00305688 Plasmid Details pMAPLe003 Bacterial expression vector with a T7 promoter, N-terminal MBP and C-terminal 6xHis tag-TEV protease site; constitutively expresses TEV protease to produce C-terminal His-tagged protein; contains the ccdB death cassette; kanamycin resistance in bacteria; ligation independent cloning (LIC). PSI
60 EvNO00306565 Plasmid Details pMYC4-H Mycoplasma coexpression expression vector with the Phsp promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning ( NdeI, SbfI). PSI
61 EvNO00306566 Plasmid Details pMYC5-H Mycoplasma coexpression expression vector with the Phsp promoter and C-termial c-myc tag; modified from pMYC4-H with unique SrfI site; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (NdeI, SbfI). PSI
62 EvNO00306567 Plasmid Details pMYC4-I Mycoplasma coexpression expression vector with the Pimyc promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PacI, SbfI). PSI
63 EvNO00306568 Plasmid Details pMYC4-S Mycoplasma coexpression expression vector with the Psmyc promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PacI, SbfI). PSI
64 EvNO00306569 Plasmid Details pMYC4-Hp Mycoplasma coexpression expression vector with the Phsp promoter and C-termial c-myc tag; newer version of pMYC4-H suitable for universal cloning by PacI and SbfI sites; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PacI, SbfI). PSI
65 EvNO00306570 Plasmid Details p4-Hb Mycoplasma coexpression expression vector with the Phsp promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (AsiSI, PmeI). PSI
66 EvNO00306571 Plasmid Details p4-Ib Mycoplasma coexpression expression vector with the Pimyc promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (AsiSI, PmeI). PSI
67 EvNO00306572 Plasmid Details p4-Sb Mycoplasma coexpression expression vector with the Psmyc promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (AsiSI, PmeI). PSI
68 EvNO00306573 Plasmid Details p9T-c Mycoplasma coexpression expression vector with the PtetO promoter; tetracycline-inducible expression of insert; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PtetO gene: AsiSI, PmeI; second constitutively expressed gene: PacI, SbfI). PSI
69 EvNO00306585 Plasmid Details p9T-H Mycoplasma coexpression expression vector with the PtetO promoter and Phsp promoter; tetracycline-inducible expression of insert; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PtetO gene: AsiSI, PmeI; Phsp gene: PacI, SbfI). PSI
70 EvNO00306605 Plasmid Details p9T-I Mycoplasma coexpression expression vector with the PtetO promoter and Pimyc promoter; tetracycline-inducible expression of insert; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PtetO gene: AsiSI, PmeI; Pimyc gene: PacI, SbfI). PSI
71 EvNO00306625 Plasmid Details p9T-S Mycoplasma coexpression expression vector with the PtetO promoter and Psmyc promoter; tetracycline-inducible expression of insert; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PtetO gene: AsiSI, PmeI; Psmyc gene: PacI, SbfI). PSI
72 EvNO00306645 Plasmid Details pVP80K Bacterial expression vector with a T5 promoter and N-terminal 8xHis-MBP tag; kanamycin resistance in bacteria; restriction enzyme cloning. PSI
73 EvNO00306665 Plasmid Details pVP81K Bacterial expression vector with a T5 promoter with an auto-cleaving (TVMV protease) N-terminal MBP tag that leaves an N-terminal 8xHis tagged protein; kanamycin resistance in bacteria; restriction enzyme cloning. PSI
74 EvNO00306666 Plasmid Details pVP91A Bacterial expression vector with a T5 promoter and N-terminal 8xHis tag; ampicilliin resistance in bacteria; restriction enzyme cloning. PSI
75 EvNO00306667 Plasmid Details pEU-HSBC In Vitro SP6 transcription and wheat germ cell free protein expression vector with a SP6 promoter and N-terminal 6xHis tag; ampicillin resistance in bacteria; restriction enzyme cloning. PSI
76 EvNO00306668 Plasmid Details pVP65K Bacterial (E. coli) expression with N-terminal 8xHis, MBP tag; kanamycin resistance in bacteria; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat cassette. PSI,Promega
77 EvNO00306669 Plasmid Details pVP68K Bacterial (E. coli) expression with N-terminal 8xHis, MBP tag; kanamycin resistance in bacteria; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat cassette. PSI,Promega
78 EvNO00336943 Plasmid Details pET15_NESG Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning. PSI/NESG
79 EvNO00336944 Plasmid Details pET21_NESG Bacterial (E. coli) expression vector with T7 lac promoter, adds C-terminal 6xHis tag; ampicillin resistance; restriction enzyme cloning. PSI/NESG
80 EvNO00340963 Plasmid Details pMCSG26 Bacterial expression vector with C-terminal 6xHis tag and T7 promoter; ampicillin resistance in bacterial; ligation independent cloning (LIC) PSI
81 EvNO00340964 Plasmid Details pMCSG28 Bacterial expression vector with T7 promoter and TEV-cleavage C-terminal 6xHis; ampicillin resistance in bacterial; ligation independent cloning (LIC) PSI
82 EvNO00340965 Plasmid Details pMCSG32 Bacterial expression vector with T7 promoter and C-terminal 6xHis, TEV-protease cleavage site, N-terminal MBP tag with TVMV site; ampicillin resistance in bacterial; ligation independent cloning (LIC) PSI
83 EvNO00430383 Plasmid Details pSGP17 Yeast expression vector with a AOX1 promoter C-terminal CBP and RSG-6xHis tag with 3C protease cleavage site and pre-pro-alpha-factor leader sequence; zeocin resistance in Pichia and in bacteria; ligation independent cloning (LIC) PSI
84 EvNO00430384 Plasmid Details pSGP18 Yeast expression vector with a AOX1 promoter C-terminal CBP and RSG-6xHis tag with 3C protease cleavage site; zeocin resistance in Pichia and in bacteria; ligation independent cloning (LIC) PSI
85 EvNO00450863 Plasmid Details pMCSG53 Bacterial expression vector with a T7 promoter and TEV-cleavable N-terminal 6xHis tag; Contains rare argU and ileX tRNA genes; ampicillin resistance in bacteria; ligation independent cloning. PSI
86 EvNO00450864 Plasmid Details pMCSG68 Bacterial expression vector with a T7 promoter and TEV-cleavable N-terminal 6xHis and StrepII tags; rare argU and ileX codons; ampicillin resistance in bacteria; ligation independent cloning PSI
87 EvNO00450865 Plasmid Details pMCSG69 Bacterial expression vector with a T7 promoter and N-terminal MBP tag, TVMV cleavage site, 6xHis tag and TEV protease cleavage site with rare codon argU and ileX tRNA genes; ampicillin resistance in bacteria; ligation independent cloning PSI
88 EvNO00454843 Plasmid Details pMCSG49 Bacterial expression vector with a T7 promoter and N-terminal 6xHis tag, TEV cleavable; ampicillin resistance in bacteria; Ligation Independent cloning PSI
89 EvNO00454845 Plasmid Details pMCSG54 Bacterial expression vector with a T7 promoter and N-terminal Flag and 6xHiis tag followed by TEV protease cleavage site ; ampicillin resistance in bacteria; ligation independent cloning. PSI
90 EvNO00454846 Plasmid Details pMCSG55 Bacterial expression vector with T7 promoter and N-terminal 6xHis and HA tags followed by TEV protease cleavage site; ampicillin resistance in bacteria; ligation independent cloning PSI
91 EvNO00454847 Plasmid Details pMCSG56 Bacterial expression vector with T7 promoter and N-terminal 6xHis and double HA tags followed by TEV protease cleavage site; ampicillin resistance in bacteria; ligation independent cloning PSI
92 EvNO00599463 Plasmid Details pMCSG58 Bacterial expression vector with T7 promoter and C-terminal 6xHis tag; contain argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning PSI
93 EvNO00599464 Plasmid Details pMCSG59 Bacterial expression vector with T7 promoter and TEV-cleavable C-terminal 6xHis tag; contain argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning PSI
94 EvNO00599484 Plasmid Details pMCSG63 Bacterial co-expresion vector with T7 promoter and N-terminal TEV-cleavable 6xHis tag; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning PSI
95 EvNO00599485 Plasmid Details pMCSG70 Bacterial expression vector with T7 promoter and C-terminal TEV-cleavable 10xHis tag and TVMV-cleavable MBP tag; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning PSI
96 EvNO00599486 Plasmid Details pMCSG71 Bacterial expression vector with T7 promoter, N-terminal TVMV-cleavable MBP tag and N-terminal TEV-cleavable 6xHis tag; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning PSI
97 EvNO00599503 Plasmid Details pMCSG75 Bacterial expression vector with T7 promoter and TEV-cleavable 6xHis tag BirA gene; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning PSI
98 EvNO00599504 Plasmid Details pMCSG76 Bacterial co-expression vector with T7 promoter and N-terminal TEV-cleavable 6xHis tag; contains argU and ileX rare tRNA; spectinomycin resistance in bacteria; ligation independent cloning PSI
99 EvNO00599523 Plasmid Details pMCSG77 Bacterial co-expression vector with T7 promoter and N-terminal TEV-cleavable 6xHis tag; contains argU and ileX rare tRNA; kanamycin resistance in bacteria; ligation independent cloning PSI
100 EvNO00623703 Plasmid Details pRSET-natGFP Bacterial expression vector with a T7 promoter; adds C-terminal GFP tag; ampicillin resistance in bacteria; ligation independent cloning PSI
101 EvNO00623704 Plasmid Details pRSET-natGFPHis Bacterial expression vector with a T7 promoter; adds C-terminal GFP-His6 tag; ampicillin resistance in bacteria; ligation independent cloning PSI
102 EvNO00629003 Plasmid Details pET-32b-TEV Bacterial expression vector with T7 promoter and N-terminal E. coli thioredoxin tag, 6xHis-tag, thrombin cleavage site, S-tag, and TEV cleavage site; ampicillin resistance in bacteria; restriction enzyme cloning PSI
103 EvNO00629004 Plasmid Details pRSET-natTEVGFPHis Bacterial expression vector with T7 promoter and C-terminal TEV-cleavavable GFP and 6xHis-tags; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI PSI
104 EvNO00629005 Plasmid Details pCMVi-LSrCOMPTT with tag/fusion/marker assay, ligation independent cloning (LIC) clone, mammalian expression expression with intron and CMV and oligomerization domain and hGH and SV40 and leader sequence and AmpR and f1 and ColE and peptide and -10/Spec-R and COMP and Tetanus Toxoid and epitope and Stop Codons and InTron For and 013_DO3_DTH21 and RBS and 014_C03_DTH22 and 2012 and HGHTER and CMV-F; ampicillin resistance in bacterial; PSI
105 EvNO00629006 Plasmid Details pCMVi-UB Vector for antibody production in mice upon genetic immunization by gene gun with CMV promoter and N-terminal mouse ubiqiutin protein with Gly76Ala mutation; ampicillin resistance in bacteria; restriction enzyme cloning PSI
106 EvNO00629007 Plasmid Details pBAD24sigNtermHis Arabinose-inducible bacterial expression with a pBAD promoter and N-terminal E. coli YtfM (aa1-21) and 6xHis-tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI PSI
107 EvNO00629008 Plasmid Details pBAD24sigCtermHis Arabinose-inducible expression in bacteria with N-terminal E. coli YtfM (aa1-21) signal sequence and C-terminal His-tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI PSI
108 EvNO00629009 Plasmid Details pRSET-YtfM-8xHis-N-term Bacterial expression with T7 promoter and N-terminal E. coli YtfM (aa1-21) signal sequence and 8xHis-tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI PSI
109 EvNO00629010 Plasmid Details pRSET-C8xHis Bacterial expression vector with T7 promoter and C-terminal 8xHis tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI PSI
110 EvNO00629011 Plasmid Details pRSET-C6xHis Bacterial expression vector with T7 promoter and C-terminal 6xHis tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI PSI
111 EvNO00631283 Plasmid Details pCMVi-LS with tag/fusion/marker assay, multiple cloning site clone, bacterial expression expression with leader sequence and MCS and intron and hGH and forward primer and AmpR and ColE and f1 and reverse primer and DTH and -10/Spec-R and Stop Codons and CMV and SV40; ampicillin resistance in bacterial; PSI
112 EvNO00631284 Plasmid Details pRSET-YtfM-AN Bacterial expression vector with T7 promoter and N-terminal E. coli YtfM (aa1-23) and 7xHis-tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI PSI
113 EvNO00631285 Plasmid Details pCDF-BAD Arabinose-inducible expression in bacteria; spectinomycin resistance in bacteria; ligation into multiple cloning site downstream of RBS: NdeI, XhoI/SciI, AscI, ZraI/AatII; or just upstream of RBS: EagI/NotI, PmeI PSI