Empty Vectors

This is a list of clones without gene inserts (empty vectors). Please log in to see all empty vectors available to you. Many other vectors are represented in the repository, which can be found using using one of our other search options or in our list of all empty vectors.

Explanation of Terms

Clone ID Vector Name Description Special
MTA		  
EvNO00023103 pANT7_cGST with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
EvNO00023110 pANT7_nHA In vitro expression vector, makes N-term HA fusion; amp resistance; recombinational cloning.
EvNO00023115 pJP1563 Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (blasticidin for mammalian selection); recombinational cloning.
EvNO00084258 pMCSG10 with tag/fusion/marker assay, multiple cloning site clone, bacterial expression, improves solubility of insert expression with lacI and LIC and AmpR and T7 and T7 term and ori and TEV and His and His 2 and GST; ampicillin resistance in bacterial; PSI
EvNO00084259 pMCSG11 Coexpression vector with T7 promoter, adds N-terminal His tag, TEV, SNA; chloramphenicol resistance; ligation independent cloning (LIC). PSI
EvNO00084260 pMCSG12 Coexpression vector with T7 promoter, adds N-terminal His tag, Sloop, TEV, SNA; chloramphenicol resistance; ligation independent cloning (LIC). PSI
EvNO00084261 pMCSG13 Coexpression vector with T7 promoter, adds N-terminal His tag, MBP, TEV, SNA; chloramphenicol resistance; ligation independent cloning (LIC). PSI
EvNO00084262 pMCSG14 Coexpression vector with T7 promoter, adds N-terminal His tag, GST, TEV, SNA; chloramphenicol resistance; ligation independent cloning (LIC). PSI
EvNO00084263 pMCSG17 Coexpression vector with T7 promoter, adds N-terminal Stag, TEV, SNA; amp resistance; ligation independent cloning (LIC). PSI
EvNO00084264 pMCSG18 Screening vector with T7 promoter, adds N-terminal His tag, TEV, LICs, GFP; amp resistance; ligation independent cloning (LIC). PSI
EvNO00084265 pMCSG19 Vector for solubility of insert and for purification, with T7 promoter, adds N-terminal MBP, TVMV, His, TEV, NA; amp resistance; ligation independent cloning (LIC). PSI
EvNO00084266 pMCSG20 Coexpression vector with T7 promoter, adds N-terminal Stag, GST, TEV, SNA; amp resistance; ligation independent cloning (LIC PSI
EvNO00084267 pMCSG21 Coexpression vector with T7 promoter, adds N-terminal His, TEV, SNA; spectinomycin resistance; ligation independent cloning (LIC). PSI
EvNO00084268 pMCSG22 Coexpression vector with T7 promoter, adds N-terminal His, Sloop, TEV, SNA; spectinomycin resistance; ligation independent cloning (LIC). PSI
EvNO00084269 pMCSG23 Coexpression vector with T7 promoter, adds N-terminal His, MBP, TEV, SNA; spectinomycin resistance; ligation independent cloning (LIC). PSI
EvNO00084270 pMCSG24 Coexpression vector with T7 promoter, adds N-terminal His, GST, TEV, SNA; spectinomycin resistance; ligation independent cloning (LIC). PSI
EvNO00084271 pMCSG7 with tag/fusion/marker assay, ligation independent cloning SspI (LIC-SspI), bacterial expression, in vitro transcription expression with AmpR and His and LIC and ori and T7 and T7 term and TEV and His 2 and LacI and lac operator; ampicillin resistance in bacterial; PSI
EvNO00084272 pMCSG8 Vector facillitating reduced protein toxicity, with T7 promoter, adds N-terminal His tag, Sloop, TEV, SNA; amp resistance; ligation independent cloning (LIC). PSI
EvNO00084273 pMCSG9 Vector with tags for solubility of insert, with T7 promoter, adds N-terminal His tag, MBP, TEV, SNA; amp resistance; ligation independent cloning (LIC). PSI
EvNO00084274 pMCSG19C Vector for solubility of insert and for purification, with T7 promoter, adds N-terminal MBP, TVMV, His, TEV, NA; amp resistance; ligation independent cloning (LIC). PSI
EvNO00084275 pMCentr1 cloning vector to link to Gateway system, with attL1-TEV-LIC site-attL2; zeocin resistance; ligation independent cloning to make pENTR vector for recombinational cloning PSI
EvNO00084276 pMCentr2 cloning vector to link to Gateway system, with attL1-TEV-LIC site-attL2; zeocin resistance; ligation independent cloning to make pENTR vector for recombinational cloning PSI
EvNO00084277 pMCentr3 destination vector to accept inserts from pENTR clones; attL1-TVMV-LIC site-attL2; kanamycin resistance; ligation independent cloning to Gateway PSI
EvNO00084278 pVP13 Bacterial (E. coli) expression, adds N-terminal S-tag, 6xHis and MBP, adds TEV site on 5p end of insert; amp resistance; recombinational cloning; Gateway cloning into this vector will remove the Gateway ccdB death casette. PSI
EvNO00084279 pVP16 Bacterial (E. coli) expression, adds N-terminal 8xHis and MBP, adds TEV site on 5p end of insert; amp resistance; recombinational cloning; Gateway cloning into this vector will remove the Gateway ccdB death casette. PSI
EvNO00084280 pVP33A Bacterial (E. coli) expression, adds N-terminal 8xHis, MBP, 3CP cleavage, tetra-cys, Tev cleavage; amp resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte. PSI,Promega
EvNO00084281 pVP56A Bacterial (E. coli) expression, adds N-terminal 8xHis, MBP, 3CP cleavage, Tev cleavage; amp resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte. PSI,Promega
EvNO00084282 pVP56K Bacterial (E. coli) expression, adds N-terminal 8xHis, MBP, 3CP cleavage, Tev cleavage; kanamycin resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte. PSI,Promega
EvNO00084283 pEU-His-FV Cell-free expression vector, adds N-terminal 6xHis tag; amp resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte. PSI,Promega
EvNO00084284 pEU-GST-FV Cell-free expression vector, adds N-terminal GST tag and 3CP cleavage site; amp resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte. PSI,Promega
EvNO00084285 pVP33K Bacterial (E. coli) expression, adds N-terminal 6xHis tag, MBP, 3CP cleavage, tetra-cys, Tev cleavage; kanamycin resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte. PSI,Promega
EvNO00085126 p11 "Bacterial (E. coli) expression vector, adds His tag, TEV cleavage site; amp resistance; restriction enzyme cloning." PSI
EvNO00085127 p15Tv-LIC Bacterial (E. coli) expression vector, adds His tag, TEV cleavage site; ampicillin resistance; recombinational cloning (InFusion, BseRI). PSI
EvNO00085128 pDB.GST Bacterial expression vector that adds GST and TEV protease site; kanamycin resistance; restriction enzyme cloning. PSI
EvNO00085130 pDB.His.MBP "Bacterial expression vector that adds 6xHis tag, MBP, TEV protease site; kanamycin resistance; restriction enzyme cloning." PSI
EvNO00085131 pDB.His.NusA Bacterial expression vector that adds N-terminal 6xHis tag, NusA, Tev protease site; kanamycin resistance; restriction enzyme cloning. PSI
EvNO00085132 pDB.His.TRX "Bacterial expression vector that adds N-terminal 6xHis tag, thioredoxin and TEV protease sites; kanamycin resistance; restriction enzyme cloning." PSI
EvNO00085133 pDB.TRX Bacterial expression vector that adds thioredoxin and TEV protease sites; kanamycin resistance; restriction enzyme cloning. PSI
EvNO00085134 pDB-HisGST "Bacterial expression vector that adds N-terminal His6 tag, GST, TEV protease site; kanamycin resistance; restriction enzyme cloning." PSI
EvNO00091443 pNYCOMPS-LIC-FH10T+ (N term) Bacterial expression vector; T7 promoter; N terminal Flag epitope and 10X His tags; tags can be removed by TEV protease; kanamycin resistance; ligation independent cloning. PSI
EvNO00285791 pSpeedET Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHHENLYFQG tag; kanamycin resistance; PIPE cloning. PSI
EvNO00285801 pBY011 with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, inducible expression, yeast expression, yeast shuttle expression with ARAC5R and Ura3 promoter and AmpR promoter and bla and ori and CAP binding site and lac promoter and lac operator and M13 reverse and attR2 and CmR and lac UV5 promoter and attR1 and GAL1, 10 and Ura3 and UAS and M13 forward; 50ug/mL of Kanamycin resistance in Pichia;
EvNO00292960 pH3c-LIC Bacterial expression vector with N-terminal 8x His tag separated from the insert by a 3C protease cleavage site; kanamycin resistance in bacteria; ligation independent cloning (LIC) PSI
EvNO00292961 pLIC-CH Bacterial expression vector with C-terminal 8x His tag separated from the insert by a TEV protease cleavage site; kanamycin resistance in bacteria; ligation independent cloning (LIC) PSI
EvNO00292962 pHM3C-LIC Bacterial expression vector with N-terminal 6xHis and MBP tags separated from the insert by a 3C protease cleavage site; kanamycin resistance in bacteria; ligation independent cloning (LIC) PSI
EvNO00304413 pHIS8 Gateway compatible bacterial expression vector with T7 promoter, N- and C-terminal His tags and thrombin cleavage site; contains ccdB death cassette that is removed after recombinational cloning; kanamycin resistance in bacteria; recombinational cloning. PSI
EvNO00304414 pHIS9 Gateway compatible bacterial expression vector with T7 promoter and N-terminal His tag; contains ccdB death cassette that is removed after recombinational cloning; kanamycin resistance in bacteria; recombinational cloning. PSI
EvNO00304415 pMIS2.1mv Gateway compatible bacterial expression destination vector with N-terminal 8xHis tag and Mistic (from B. subtilis) tag; contains ccdB death cassette that is removed after recombinational cloning; ampicillin resistance in bacteria; recombinational cloning. PSI
EvNO00304416 pSGP46 Yeast expression vector with Adh2 promoter, adds C-terminal 3C-ZZ-10XHis tag; ampicillin resistance (bacteria); uracil resistance (yeast); ligation independent cloning (LIC). PSI
EvNO00304417 pSGP47 Yeast expression vector with Adh2 promoter, adds 3C protease cleavage site and 10xHis tag; ampicillin resistance (bacteria); uracil (yeast); ligation independent cloning (LIC). PSI
EvNO00304418 pSGP36 Yeast expression vector with Gal1 promoter, adds C-terminal 3C-his10 tag; amp resistance (URA for yeast); ligation independent cloning (LIC). PSI
EvNO00304419 pSGP40 Yeast expression vector with Gal1 promoter, adds C-terminal 3C-ZZ-His10 tag; amp resistance (URA for yeast); ligation independent cloning (LIC). PSI
EvNO00304420 pNYCOMPS-TH-ccdB (C-term.) Bacterial (e. coli) expression vector with T7 promoter, C-terminal 10xHis tag and TEV protease cleavage site; contains ccdB death cassette which is removed after LIC cloning; kanamycin resistance in bacteria; ligation independent cloning. PSI
EvNO00305686 pMA507 E. coli expression vector with a T7 promoter, N-terminal 6xHis tag and TEV protease cleavage site; contains the ccdB death cassette; kanamycin resistance in bacteria; ligation independent cloning (LIC). PSI
EvNO00305687 pMA510 Bacterial expression vector with a T7 promoter, N-terminal MBP fusion-6xHis tag-TEV protease site; contains the ccdB death cassette; kanamycin resistance in bacteria; ligation independent cloning (LIC).   PSI
EvNO00305688 pMAPLe003 Bacterial expression vector with a T7 promoter, N-terminal MBP and C-terminal 6xHis tag-TEV protease site; constitutively expresses TEV protease to produce C-terminal His-tagged protein; contains the ccdB death cassette; kanamycin resistance in bacteria; ligation independent cloning (LIC). PSI
EvNO00306565 pMYC4-H Mycoplasma coexpression expression vector with the Phsp promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning ( NdeI, SbfI). PSI
EvNO00306566 pMYC5-H Mycoplasma coexpression expression vector with the Phsp promoter and C-termial c-myc tag; modified from pMYC4-H with unique SrfI site; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (NdeI, SbfI). PSI
EvNO00306567 pMYC4-I Mycoplasma coexpression expression vector with the Pimyc promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PacI, SbfI). PSI
EvNO00306568 pMYC4-S Mycoplasma coexpression expression vector with the Psmyc promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PacI, SbfI). PSI
EvNO00306569 pMYC4-Hp Mycoplasma coexpression expression vector with the Phsp promoter and C-termial c-myc tag; newer version of pMYC4-H suitable for universal cloning by PacI and SbfI sites; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PacI, SbfI). PSI
EvNO00306570 p4-Hb Mycoplasma coexpression expression vector with the Phsp promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (AsiSI, PmeI). PSI
EvNO00306571 p4-Ib Mycoplasma coexpression expression vector with the Pimyc promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (AsiSI, PmeI). PSI
EvNO00306572 p4-Sb Mycoplasma coexpression expression vector with the Psmyc promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (AsiSI, PmeI). PSI
EvNO00306573 p9T-c Mycoplasma coexpression expression vector with the PtetO promoter; tetracycline-inducible expression of insert; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PtetO gene: AsiSI, PmeI; second constitutively expressed gene: PacI, SbfI). PSI
EvNO00306585 p9T-H Mycoplasma coexpression expression vector with the PtetO promoter and Phsp promoter; tetracycline-inducible expression of insert; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PtetO gene: AsiSI, PmeI; Phsp gene: PacI, SbfI). PSI
EvNO00306605 p9T-I Mycoplasma coexpression expression vector with the PtetO promoter and Pimyc promoter; tetracycline-inducible expression of insert; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PtetO gene: AsiSI, PmeI; Pimyc gene: PacI, SbfI). PSI
EvNO00306625 p9T-S Mycoplasma coexpression expression vector with the PtetO promoter and Psmyc promoter; tetracycline-inducible expression of insert; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PtetO gene: AsiSI, PmeI; Psmyc gene: PacI, SbfI). PSI
EvNO00306645 pVP80K Bacterial expression vector with a T5 promoter and N-terminal 8xHis-MBP tag; kanamycin resistance in bacteria; restriction enzyme cloning. PSI
EvNO00306665 pVP81K Bacterial expression vector with a T5 promoter with an auto-cleaving (TVMV protease) N-terminal MBP tag that leaves an N-terminal 8xHis tagged protein; kanamycin resistance in bacteria; restriction enzyme cloning. PSI
EvNO00306666 pVP91A Bacterial expression vector with a T5 promoter and N-terminal 8xHis tag; ampicilliin resistance in bacteria; restriction enzyme cloning. PSI
EvNO00306667 pEU-HSBC In Vitro SP6 transcription and wheat germ cell free protein expression vector with a SP6 promoter and N-terminal 6xHis tag; ampicillin resistance in bacteria; restriction enzyme cloning. PSI
EvNO00306668 pVP65K Bacterial (E. coli) expression with N-terminal 8xHis, MBP tag; kanamycin resistance in bacteria; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat cassette. PSI,Promega
EvNO00306669 pVP68K Bacterial (E. coli) expression with N-terminal 8xHis, MBP tag; kanamycin resistance in bacteria; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat cassette. PSI,Promega
EvNO00335423 ZM552 Yeast expression vector with N-terminal 3X V5 epitope tag; ampicillin resistance in bacteria; LEU2 resistance in yeast; Gateway cloning.
EvNO00336943 pET15_NESG Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning. PSI/NESG
EvNO00336944 pET21_NESG Bacterial (E. coli) expression vector with T7 lac promoter, adds C-terminal 6xHis tag; ampicillin resistance; restriction enzyme cloning. PSI/NESG
EvNO00340963 pMCSG26 Bacterial expression vector with C-terminal 6xHis tag and T7 promoter; ampicillin resistance in bacterial; ligation independent cloning (LIC) PSI
EvNO00340964 pMCSG28 Bacterial expression vector with T7 promoter and TEV-cleavage C-terminal 6xHis; ampicillin resistance in bacterial; ligation independent cloning (LIC) PSI
EvNO00340965 pMCSG32 Bacterial expression vector with T7 promoter and C-terminal 6xHis, TEV-protease cleavage site, N-terminal MBP tag with TVMV site; ampicillin resistance in bacterial; ligation independent cloning (LIC) PSI
EvNO00412303 pCPD_nGST-SBP_DC Bacterial expression vector with a T7 promoter, N-terminal GST, Thrombin prtease cleavage site, SBP tag, and TEV protease cleavage site; ampicillin and chloamphenicol resistance in bacteria (CmR removed in clones with inserts); Gateway recombinational cloning.
EvNO00412304 pCPD_nMBP-SBP_DC with tag/fusion/marker assay, Gateway&reg, acceptor (destination), recombinational cloning clone, bacterial expression expression with pCPD5698_F and pCPD6268_R and MBP and Thrombin and SBP and TEV and ccdB and CmR and T7 term and LacI and AmpR and ColE and T7 and lac operator and RBS; chloramphenicol resistance in bacterial, ampicillin resistance in bacterial;
EvNO00413803 pCPD_nSBP-Halo_DC Bacterial expression vector with a T7 promoter and N-terminal SBP and Halo tags, seperated by protease cleavage sites for thrombin and TEV protease; ampicillin resistance in bacteria; Gateway cloning HALO
EvNO00413804 pCPD_nHalo_DC Bacterial expression vector with a T7 promoter and TEV cleavable N-terminal Halo tag; ampicillin resistance in bacteria; Gateway cloning HALO
EvNO00424503 pJFT7_nHALO_DC In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable HALO tag; ampicillin resistance; Gateway or Flexi cloning compatible HALO
EvNO00424523 pJFT7_cHalo_DC In vitro expression vector with T7 promoter and C-terminal TEV-cleavable Halo tag; ampicillin resistance; Gateway or Flexi cloning compatible HALO
EvNO00424543 pJFT7_nHalo_empty In vitro expression vector with T7 promoter and N-terminal TEV-cleavable Halo tag; does not contain a death cassette; ampicillin resistance; restriction enzyme cloning HALO
EvNO00424544 pJFT7_cHalo_empty In vitro expression vector with T7 promoter and C-terminal TEV-cleavable Halo tag; does not contain a death cassette; ampicillin resistance; restriction enzyme cloning HALO
EvNO00430383 pSGP17 Yeast expression vector with a AOX1 promoter C-terminal CBP and RSG-6xHis tag with 3C protease cleavage site and pre-pro-alpha-factor leader sequence; zeocin resistance in Pichia and in bacteria; ligation independent cloning (LIC) PSI
EvNO00430384 pSGP18 Yeast expression vector with a AOX1 promoter C-terminal CBP and RSG-6xHis tag with 3C protease cleavage site; zeocin resistance in Pichia and in bacteria; ligation independent cloning (LIC) PSI
EvNO00450863 pMCSG53 Bacterial expression vector with a T7 promoter and TEV-cleavable N-terminal 6xHis tag; Contains rare argU and ileX tRNA genes; ampicillin resistance in bacteria; ligation independent cloning. PSI
EvNO00450864 pMCSG68 Bacterial expression vector with a T7 promoter and TEV-cleavable N-terminal 6xHis and StrepII tags; rare argU and ileX codons; ampicillin resistance in bacteria; ligation independent cloning PSI
EvNO00450865 pMCSG69 Bacterial expression vector with a T7 promoter and N-terminal MBP tag, TVMV cleavage site, 6xHis tag and TEV protease cleavage site with rare codon argU and ileX tRNA genes; ampicillin resistance in bacteria; ligation independent cloning PSI
EvNO00450883 pJSP6_nHalo_DC Wheat germ expression vector with a SP6 promoter and N-terminal TEV-cleavable HALO tag; ampicillin resistance in bacteria and chloramphenicol resistance in empty vector; Gateway or Flexi cloning HALO
EvNO00450884 pJSP6_nHalo_empty Wheat germ expression vector with SP6 promoter and TEV-cleavable N-terminal HALO tag; ampicillin resistance in bacteria; gateway cloning HALO
EvNO00454843 pMCSG49 Bacterial expression vector with a T7 promoter and N-terminal 6xHis tag, TEV cleavable; ampicillin resistance in bacteria; Ligation Independent cloning PSI
EvNO00454845 pMCSG54 Bacterial expression vector with a T7 promoter and N-terminal Flag and 6xHiis tag followed by TEV protease cleavage site ; ampicillin resistance in bacteria; ligation independent cloning. PSI
EvNO00454846 pMCSG55 Bacterial expression vector with T7 promoter and N-terminal 6xHis and HA tags followed by TEV protease cleavage site; ampicillin resistance in bacteria; ligation independent cloning PSI
EvNO00454847 pMCSG56 Bacterial expression vector with T7 promoter and N-terminal 6xHis and double HA tags followed by TEV protease cleavage site; ampicillin resistance in bacteria; ligation independent cloning PSI
EvNO00534623 pGEn1-DEST Mammalian expression vector with a CMV promoter and N-terminal TCM 8xHis and Strep tag; ampicillin resistance in bacteria; Gateway recombinational cloning SPTA
EvNO00534624 pGEn2-DEST Mammalian expression vector with a CMV promoter and N-terminal 8xHis, AviTag, and Super GFP tags; ampicillin resistance in bacteria; Gateway recombinational cloning SPTA
EvNO00534626 pGEc1-DEST Mammalian expression vector with a CMV promoter and C-terminal 8xHis and Strep tags; ampicillin resistance in bacteria; Gateway recombinational cloning SPTA
EvNO00534627 pGEc2-DEST Mammalian expression vector with a CMV promoter and C-terminal Superfolder GFP, AviTag and 10xHis tag; ampicillin resistance in bacteria; Gateway recombinational cloning SPTA
EvNO00548203 pET16-DEST Bacterial expression vector with a T7 promoter and a N-terminal 10xHis tag; ampicillin resistance in bacteria; Gateway recombinational cloning. SPTA
EvNO00548204 pET32-DEST Bacterial expression vector with T7 promoter and N-terminal Trx, 6xHis and S-tags; Thrombin and enterokinase cleavage sites; ampicillin resistance in bacteria; Gateway recombinational cloning SPTA
EvNO00587843 pJSP6_nGST_DC Wheat germ expression vector with a SP6 promoter and N-terminal TEV-cleavable GST tag; ampicillin resistance; Gateway or Flexi cloning compatible
EvNO00595403 pJFT7_nHIS_cHalo_DC In vitro expression vector with a T7 promoter and N-terminal HIS and C-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible HALO
EvNO00595404 pJFT7_cHalo-3xHA_DC In vitro expression vector with a T7 promoter and C-terminal TEV-cleavable HALO-3xHA tag; ampicillin resistance; Gateway or Flexi cloning compatible HALO
EvNO00595405 pJFT7_nFlag_cHalo_DC In vitro expression vector with a T7 promoter and N-terminal Flag and C-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible HALO
EvNO00595406 pJFT7_nHA_cHalo_DC In vitro expression vector with a T7 promoter and N-terminal HA and C-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible HALO
EvNO00595407 pJFT7_n3xFlag-Halo_DC In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable 3xFlag-Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible HALO
EvNO00595408 pJFT7_cHalo-3xFlag_DC In vitro expression vector with a T7 promoter and C-terminal TEV-cleavable HALO-3xFlag tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible HALO
EvNO00595409 pJFT7_n3xHA-Halo_DC In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable 3xHA-Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible HALO
EvNO00599463 pMCSG58 Bacterial expression vector with T7 promoter and C-terminal 6xHis tag; contain argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning PSI
EvNO00599464 pMCSG59 Bacterial expression vector with T7 promoter and TEV-cleavable C-terminal 6xHis tag; contain argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning PSI
EvNO00599484 pMCSG63 Bacterial co-expresion vector with T7 promoter and N-terminal TEV-cleavable 6xHis tag; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning PSI
EvNO00599485 pMCSG70 Bacterial expression vector with T7 promoter and C-terminal TEV-cleavable 10xHis tag and TVMV-cleavable MBP tag; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning PSI
EvNO00599486 pMCSG71 Bacterial expression vector with T7 promoter, N-terminal TVMV-cleavable MBP tag and N-terminal TEV-cleavable 6xHis tag; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning PSI
EvNO00599503 pMCSG75 Bacterial expression vector with T7 promoter and TEV-cleavable 6xHis tag BirA gene; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning PSI
EvNO00599504 pMCSG76 Bacterial co-expression vector with T7 promoter and N-terminal TEV-cleavable 6xHis tag; contains argU and ileX rare tRNA; spectinomycin resistance in bacteria; ligation independent cloning PSI
EvNO00599523 pMCSG77 Bacterial co-expression vector with T7 promoter and N-terminal TEV-cleavable 6xHis tag; contains argU and ileX rare tRNA; kanamycin resistance in bacteria; ligation independent cloning PSI
EvNO00601503 pLIFE-3'UTR Mammalian expression vector for detecting the expression of 3'UTRs by Firefly luciferase; also contains Renilla luciferase for normalization; ampicillin resistance in bacteria; Gateway cloning with P2RP3 Gateway Cassette SPTA
EvNO00601504 pLIFE-miRNA Mammalian expression vector for expressing miRNAs; also contains red fluorescent protein, gateway cassette located within an intron of RFP 3'UTR, for ncRNA expression; ampicillin resistance in bacteria; Gateway cloning with L2R3 Gateway Cassette SPTA
EvNO00601603 pCMV(delta1) Mammalian expression vector with a deletion mutant of the CMV promoter; ampicillin resistance in bacteria, neomycin resistance in mammalian cells; restriction enzyme cloning SPTA
EvNO00601604 pCMV(delta2) Mammalian expression vector with a deletion mutant of the CMV promoter; ampicillin resistance in bacteria, neomycin resistance in mammalian cells; restriction enzyme cloning SPTA
EvNO00601605 pCMV(delta3) Mammalian expression vector with a deletion mutant of the CMV promoter; ampicillin resistance in bacteria, neomycin resistance in mammalian cells; restriction enzyme cloning SPTA
EvNO00601606 pCMV(delta4) Mammalian expression vector with a deletion mutant of the CMV promoter; ampicillin resistance in bacteria, neomycin resistance in mammalian cells; restriction enzyme cloning SPTA
EvNO00601607 pCMV(delta5) Mammalian expression vector with a deletion mutant of the CMV promoter; ampicillin resistance in bacteria, neomycin resistance in mammalian cells; restriction enzyme cloning SPTA
EvNO00601608 pCMV(delta6) Mammalian expression vector with a deletion mutant of the CMV promoter; ampicillin resistance in bacteria, neomycin resistance in mammalian cells; restriction enzyme cloning SPTA
EvNO00601609 pCMV(WT) Mammalian expression vector with a CMV promoter; ampicillin resistance in bacteria, neomycin resisatance in mammalian cells; restriction enzyme cloning SPTA
EvNO00621023 pCPD_nHalo_cHIS_DC Bacterial expression with a T7 promoter, N-terminal TEV-cleavable HaloTag and C-terminal 3C protease cleavable 6xHis tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible HALO
EvNO00621024 pJFT7_nHalo_DC(r3) In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible HALO
EvNO00623103 pJFT7_nGST_DC-r2 In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
EvNO00623703 pRSET-natGFP Bacterial expression vector with a T7 promoter; adds C-terminal GFP tag; ampicillin resistance in bacteria; ligation independent cloning PSI
EvNO00623704 pRSET-natGFPHis Bacterial expression vector with a T7 promoter; adds C-terminal GFP-His6 tag; ampicillin resistance in bacteria; ligation independent cloning PSI
EvNO00623723 pJFT7_nHalo_DC(r4) In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible HALO
EvNO00623724 pJSP6_cHalo_DC Wheat germ expression vector with SP6 promoter and TEV-cleavable C-terminal HALO tag; ampicillin resistance in bacteria; gateway cloning HALO
EvNO00629003 pET-32b-TEV Bacterial expression vector with T7 promoter and N-terminal E. coli thioredoxin tag, 6xHis-tag, thrombin cleavage site, S-tag, and TEV cleavage site; ampicillin resistance in bacteria; restriction enzyme cloning PSI
EvNO00629004 pRSET-natTEVGFPHis Bacterial expression vector with T7 promoter and C-terminal TEV-cleavavable GFP and 6xHis-tags; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI PSI
EvNO00629005 pCMVi-LSrCOMPTT with tag/fusion/marker assay, ligation independent cloning (LIC) clone, mammalian expression expression with intron and CMV and oligomerization domain and hGH and SV40 and leader sequence and AmpR and f1 and ColE and peptide and -10/Spec-R and COMP and Tetanus Toxoid and epitope and Stop Codons and InTron For and 013_DO3_DTH21 and RBS and 014_C03_DTH22 and 2012 and HGHTER and CMV-F; ampicillin resistance in bacterial; PSI
EvNO00629006 pCMVi-UB Vector for antibody production in mice upon genetic immunization by gene gun with CMV promoter and N-terminal mouse ubiqiutin protein with Gly76Ala mutation; ampicillin resistance in bacteria; restriction enzyme cloning PSI
EvNO00629007 pBAD24sigNtermHis Arabinose-inducible bacterial expression with a pBAD promoter and N-terminal E. coli YtfM (aa1-21) and 6xHis-tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI PSI
EvNO00629008 pBAD24sigCtermHis Arabinose-inducible expression in bacteria with N-terminal E. coli YtfM (aa1-21) signal sequence and C-terminal His-tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI PSI
EvNO00629009 pRSET-YtfM-8xHis-N-term Bacterial expression with T7 promoter and N-terminal E. coli YtfM (aa1-21) signal sequence and 8xHis-tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI PSI
EvNO00629010 pRSET-C8xHis Bacterial expression vector with T7 promoter and C-terminal 8xHis tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI PSI
EvNO00629011 pRSET-C6xHis Bacterial expression vector with T7 promoter and C-terminal 6xHis tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI PSI
EvNO00631283 pCMVi-LS with tag/fusion/marker assay, multiple cloning site clone, bacterial expression expression with leader sequence and MCS and intron and hGH and forward primer and AmpR and ColE and f1 and reverse primer and DTH and -10/Spec-R and Stop Codons and CMV and SV40; ampicillin resistance in bacterial; PSI
EvNO00631284 pRSET-YtfM-AN Bacterial expression vector with T7 promoter and N-terminal E. coli YtfM (aa1-23) and 7xHis-tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI PSI
EvNO00631285 pCDF-BAD Arabinose-inducible expression in bacteria; spectinomycin resistance in bacteria; ligation into multiple cloning site downstream of RBS: NdeI, XhoI/SciI, AscI, ZraI/AatII; or just upstream of RBS: EagI/NotI, PmeI PSI
EvNO00665463 pANT7_GST-control In vitro expression vector expressing two GSTs as a control vector for clones in pANT7_cGST; ampicillin resistance in bacteria; recombinational cloning.
EvNO00813763 pPPL ligation independent cloning (LIC) clone with pBR322 origin and Ampicillin Resistance and ROP and ORF frame 2 – Tetracycline resistance and ORF frame 1 and pBRrevBam and T7 and T7 term primer and BseRI Stuffer and MBP signal sequence (M1-A26 of NP_290668) and RBS and MBP (K27-T392 of NP_290668) and HIS10 and TEV and FC sequencing primer pET26 pelB seq R and FC sequencing primer MBP pelB seqF and FC sequencing primer pET-PPL seq 1 F and FC sequencing primer pET-PPL seq 1 R and FC sequencing primer pET-PPL seq 2 F and T7 term and FC sequencing primer pET-PPL seq 2 R and AmpR; ampicillin resistance in bacterial;
EvNO00813764 pET-BAD ligation independent cloning (LIC) clone, bacterial expression expression with Operator O2 and araC promoter and T7 term and Operator O1 and B-lactamase gene and CAP site and T7 term primer and pBR322 origin and Operator I1 and I2 and arabinose BAD promoter and rrnB T1 T2 and RBS and ARAC3F Primer and PCBSREV Primer and ORICOL Primer and BADT1A Primer and BADT1B Primer and AMP5R Primer and -35 AP and F1ORI Primer and ARAC5R and -10 AP and rep_origin and ROP and araC; ampicillin resistance in bacterial;
EvNO00813765 pRSF-BAD ligation independent cloning (LIC) clone, bacterial expression expression with Kan-R and T7TER primer (#35) and T7 Terminator and RNA II and RNA I and RSF ori - RSF1030 Homology and -10 RNA I and -35 RNA I and -35 AP and -10 AP and RBS-reverse-weak and araC and Operator O2 and araC promoter and Operator O1 and CAP site and Operator I1 and I2 and arabinose BAD promoter and rrnB T1 T2 and RBS and ligation junction Xbal and BADT1A Primer and BADT1B Primer and ARAC3F primer (#145) and ARAC5R primer (#148) and wrong primer #62 off by 3 nt and KAN1F primer (#120) and ligation junction Pacl; 50ug/mL of Kanamycin resistance in bacterial;
EvNO00813783 pelB-MBP ligation independent cloning (LIC) clone, bacterial expression expression with RBS (extended) and TET-2 and pelB and pBR322 origin and KanR and ROP and BseRI and HIS10 and MBP and TEV and FC sequencing primer pET26 pelB seq R and FC sequencing primer MBP pelB seqF and TET and LacI and FC sequencing primer pET-PPL seq 1 F and FC sequencing primer pET-PPL seq 1 R and FC sequencing primer pET-PPL seq 2 F and pBRrevBam and T7 and lac operator and T7 term primer; kanamycin resistance in bacterial;
EvNO00813803 pCPD-pRL-CMV-ccdb fluorescent marker assay, Gateway cloning, bacterial expression expression with Renilla Luciferase and pCEP Fwd Primer and ccdB and CMV and CAGF and CMV-F and Amp-R and lac promoter and attR1 and attR2 and T7 promoter and Chloramphenicol-R; chloramphenicol resistance in bacterial, ampicillin resistance in bacterial;
EvNO00846203 pPSU1 High copy number plasmid which provides 500, 1000, 1500, 2000 and 5000 bp EcoRV fragments of kilobase DNA ladder, and 500, 700, 800, 900, 1000, 2000 and 4100 bp PstI fragments of 100 bp DNA ladder. Confers ampicillin resistance.
EvNO00846223 pPSU2 High copy number plasmid which provides 750, 3000 and 4000 bp EcoRV fragments of kilobase DNA ladder, and 50, 100, 200, 300, 400, 500, 600, 1500 and 4100 bp PstI fragments of 100 bp DNA ladder. Confers ampicillin resistance.
EvNO00877063 pLX304 with tag/fusion/marker assay, Gateway&reg, acceptor (destination), recombinational cloning clone, lentiviral infection expression with BlastR and T7 and attR1 and CMV and cPPT and V5 and AmpR and attR2 and CAT and ccdB and WPRE; ampicillin resistance in bacterial, blasticidin resistance in mammalian;
EvNO00877064 pLV_R4R2_GTW3 with tag/fusion/marker assay, Gateway&reg clone, retroviral vector expression with RRE; chloramphenicol resistance in bacterial, ampicillin resistance in bacterial, blasticidin resistance in mammalian;
EvNO00877066 pMK-Dest with tag/fusion/marker assay, Gateway&reg clone, bacterial expression expression with CMV and CMV-F and attR1 and attR2 and lac promoter and pBR322 origin and NeoR and AttB2 and TEV; kanamycin resistance in bacterial, neomycin resistance in bacterial;
The Biodesign Institute at Arizona State University

DNASU Plasmid Repository

The Biodesign Institute/Arizona State University

PO Box 878008, Tempe, AZ 85287-6401 | Map

General Customer Service - Phone: (480) 965-5697 • Email: DNASUHelp@asu.edu

Payment Questions - Phone: (480) 965-4544 • Email: DNASUPay@asu.edu

Page Contact: DNASU help | Biodesign Institute

Copyright & trademark | Accessibility | Privacy | Emergency | Contact DNASU