Clone ID
|
Vector Name
|
Description |
Special MTA |
|
EvNO00023103
|
pANT7_cGST
|
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
|
|
EvNO00023110
|
pANT7_nHA
|
In vitro expression vector, makes N-term HA fusion; amp resistance; recombinational cloning.
|
|
EvNO00023115
|
pJP1563
|
Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (blasticidin for mammalian selection); recombinational cloning.
|
|
EvNO00084258
|
pMCSG10
|
with tag/fusion/marker assay, multiple cloning site clone, bacterial expression, improves solubility of insert expression with lacI and LIC and AmpR and T7 and T7 term and ori and TEV and His and His 2 and GST; ampicillin resistance in bacterial;
|
PSI
|
EvNO00084259
|
pMCSG11
|
Coexpression vector with T7 promoter, adds N-terminal His tag, TEV, SNA; chloramphenicol resistance; ligation independent cloning (LIC).
|
PSI
|
EvNO00084260
|
pMCSG12
|
Coexpression vector with T7 promoter, adds N-terminal His tag, Sloop, TEV, SNA; chloramphenicol resistance; ligation independent cloning (LIC).
|
PSI
|
EvNO00084261
|
pMCSG13
|
Coexpression vector with T7 promoter, adds N-terminal His tag, MBP, TEV, SNA; chloramphenicol resistance; ligation independent cloning (LIC).
|
PSI
|
EvNO00084262
|
pMCSG14
|
Coexpression vector with T7 promoter, adds N-terminal His tag, GST, TEV, SNA; chloramphenicol resistance; ligation independent cloning (LIC).
|
PSI
|
EvNO00084263
|
pMCSG17
|
Coexpression vector with T7 promoter, adds N-terminal Stag, TEV, SNA; amp resistance; ligation independent cloning (LIC).
|
PSI
|
EvNO00084264
|
pMCSG18
|
Screening vector with T7 promoter, adds N-terminal His tag, TEV, LICs, GFP; amp resistance; ligation independent cloning (LIC).
|
PSI
|
EvNO00084265
|
pMCSG19
|
Vector for solubility of insert and for purification, with T7 promoter, adds N-terminal MBP, TVMV, His, TEV, NA; amp resistance; ligation independent cloning (LIC).
|
PSI
|
EvNO00084266
|
pMCSG20
|
Coexpression vector with T7 promoter, adds N-terminal Stag, GST, TEV, SNA; amp resistance; ligation independent cloning (LIC
|
PSI
|
EvNO00084267
|
pMCSG21
|
Coexpression vector with T7 promoter, adds N-terminal His, TEV, SNA; spectinomycin resistance; ligation independent cloning (LIC).
|
PSI
|
EvNO00084268
|
pMCSG22
|
Coexpression vector with T7 promoter, adds N-terminal His, Sloop, TEV, SNA; spectinomycin resistance; ligation independent cloning (LIC).
|
PSI
|
EvNO00084269
|
pMCSG23
|
Coexpression vector with T7 promoter, adds N-terminal His, MBP, TEV, SNA; spectinomycin resistance; ligation independent cloning (LIC).
|
PSI
|
EvNO00084270
|
pMCSG24
|
Coexpression vector with T7 promoter, adds N-terminal His, GST, TEV, SNA; spectinomycin resistance; ligation independent cloning (LIC).
|
PSI
|
EvNO00084271
|
pMCSG7
|
with tag/fusion/marker assay, ligation independent cloning SspI (LIC-SspI), bacterial expression, in vitro transcription expression with AmpR and His and LIC and ori and T7 and T7 term and TEV and His 2 and LacI and lac operator; ampicillin resistance in bacterial;
|
PSI
|
EvNO00084272
|
pMCSG8
|
Vector facillitating reduced protein toxicity, with T7 promoter, adds N-terminal His tag, Sloop, TEV, SNA; amp resistance; ligation independent cloning (LIC).
|
PSI
|
EvNO00084273
|
pMCSG9
|
Vector with tags for solubility of insert, with T7 promoter, adds N-terminal His tag, MBP, TEV, SNA; amp resistance; ligation independent cloning (LIC).
|
PSI
|
EvNO00084274
|
pMCSG19C
|
Vector for solubility of insert and for purification, with T7 promoter, adds N-terminal MBP, TVMV, His, TEV, NA; amp resistance; ligation independent cloning (LIC).
|
PSI
|
EvNO00084275
|
pMCentr1
|
cloning vector to link to Gateway system, with attL1-TEV-LIC site-attL2; zeocin resistance; ligation independent cloning to make pENTR vector for recombinational cloning
|
PSI
|
EvNO00084276
|
pMCentr2
|
cloning vector to link to Gateway system, with attL1-TEV-LIC site-attL2; zeocin resistance; ligation independent cloning to make pENTR vector for recombinational cloning
|
PSI
|
EvNO00084277
|
pMCentr3
|
destination vector to accept inserts from pENTR clones; attL1-TVMV-LIC site-attL2; kanamycin resistance; ligation independent cloning to Gateway
|
PSI
|
EvNO00084278
|
pVP13
|
Bacterial (E. coli) expression, adds N-terminal S-tag, 6xHis and MBP, adds TEV site on 5p end of insert; amp resistance; recombinational cloning; Gateway cloning into this vector will remove the Gateway ccdB death casette.
|
PSI
|
EvNO00084279
|
pVP16
|
Bacterial (E. coli) expression, adds N-terminal 8xHis and MBP, adds TEV site on 5p end of insert; amp resistance; recombinational cloning; Gateway cloning into this vector will remove the Gateway ccdB death casette.
|
PSI
|
EvNO00084280
|
pVP33A
|
Bacterial (E. coli) expression, adds N-terminal 8xHis, MBP, 3CP cleavage, tetra-cys, Tev cleavage; amp resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte.
|
PSI,Promega
|
EvNO00084281
|
pVP56A
|
Bacterial (E. coli) expression, adds N-terminal 8xHis, MBP, 3CP cleavage, Tev cleavage; amp resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte.
|
PSI,Promega
|
EvNO00084282
|
pVP56K
|
Bacterial (E. coli) expression, adds N-terminal 8xHis, MBP, 3CP cleavage, Tev cleavage; kanamycin resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte.
|
PSI,Promega
|
EvNO00084283
|
pEU-His-FV
|
Cell-free expression vector, adds N-terminal 6xHis tag; amp resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte.
|
PSI,Promega
|
EvNO00084284
|
pEU-GST-FV
|
Cell-free expression vector, adds N-terminal GST tag and 3CP cleavage site; amp resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte.
|
PSI,Promega
|
EvNO00084285
|
pVP33K
|
Bacterial (E. coli) expression, adds N-terminal 6xHis tag, MBP, 3CP cleavage, tetra-cys, Tev cleavage; kanamycin resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte.
|
PSI,Promega
|
EvNO00085126
|
p11
|
"Bacterial (E. coli) expression vector, adds His tag, TEV cleavage site; amp resistance; restriction enzyme cloning."
|
PSI
|
EvNO00085127
|
p15Tv-LIC
|
Bacterial (E. coli) expression vector, adds His tag, TEV cleavage site; ampicillin resistance; recombinational cloning (InFusion, BseRI).
|
PSI
|
EvNO00085128
|
pDB.GST
|
Bacterial expression vector that adds GST and TEV protease site; kanamycin resistance; restriction enzyme cloning.
|
PSI
|
EvNO00085130
|
pDB.His.MBP
|
"Bacterial expression vector that adds 6xHis tag, MBP, TEV protease site; kanamycin resistance; restriction enzyme cloning."
|
PSI
|
EvNO00085131
|
pDB.His.NusA
|
Bacterial expression vector that adds N-terminal 6xHis tag, NusA, Tev protease site; kanamycin resistance; restriction enzyme cloning.
|
PSI
|
EvNO00085132
|
pDB.His.TRX
|
"Bacterial expression vector that adds N-terminal 6xHis tag, thioredoxin and TEV protease sites; kanamycin resistance; restriction enzyme cloning."
|
PSI
|
EvNO00085133
|
pDB.TRX
|
Bacterial expression vector that adds thioredoxin and TEV protease sites; kanamycin resistance; restriction enzyme cloning.
|
PSI
|
EvNO00085134
|
pDB-HisGST
|
"Bacterial expression vector that adds N-terminal His6 tag, GST, TEV protease site; kanamycin resistance; restriction enzyme cloning."
|
PSI
|
EvNO00091443
|
pNYCOMPS-LIC-FH10T+ (N term)
|
Bacterial expression vector; T7 promoter; N terminal Flag epitope and 10X His tags; tags can be removed by TEV protease; kanamycin resistance; ligation independent cloning.
|
PSI
|
EvNO00285791
|
pSpeedET
|
Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHHENLYFQG tag; kanamycin resistance; PIPE cloning.
|
PSI
|
EvNO00285801
|
pBY011
|
with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, inducible expression, yeast expression, yeast shuttle expression with ARAC5R and Ura3 promoter and AmpR promoter and bla and ori and CAP binding site and lac promoter and lac operator and M13 reverse and attR2 and CmR and lac UV5 promoter and attR1 and GAL1, 10 and Ura3 and UAS and M13 forward; 50ug/mL of Kanamycin resistance in Pichia;
|
|
EvNO00292960
|
pH3c-LIC
|
Bacterial expression vector with N-terminal 8x His tag separated from the insert by a 3C protease cleavage site; kanamycin resistance in bacteria; ligation independent cloning (LIC)
|
PSI
|
EvNO00292961
|
pLIC-CH
|
Bacterial expression vector with C-terminal 8x His tag separated from the insert by a TEV protease cleavage site; kanamycin resistance in bacteria; ligation independent cloning (LIC)
|
PSI
|
EvNO00292962
|
pHM3C-LIC
|
Bacterial expression vector with N-terminal 6xHis and MBP tags separated from the insert by a 3C protease cleavage site; kanamycin resistance in bacteria; ligation independent cloning (LIC)
|
PSI
|
EvNO00304413
|
pHIS8
|
Gateway compatible bacterial expression vector with T7 promoter, N- and C-terminal His tags and thrombin cleavage site; contains ccdB death cassette that is removed after recombinational cloning; kanamycin resistance in bacteria; recombinational cloning.
|
PSI
|
EvNO00304414
|
pHIS9
|
Gateway compatible bacterial expression vector with T7 promoter and N-terminal His tag; contains ccdB death cassette that is removed after recombinational cloning; kanamycin resistance in bacteria; recombinational cloning.
|
PSI
|
EvNO00304415
|
pMIS2.1mv
|
Gateway compatible bacterial expression destination vector with N-terminal 8xHis tag and Mistic (from B. subtilis) tag; contains ccdB death cassette that is removed after recombinational cloning; ampicillin resistance in bacteria; recombinational cloning.
|
PSI
|
EvNO00304416
|
pSGP46
|
Yeast expression vector with Adh2 promoter, adds C-terminal 3C-ZZ-10XHis tag; ampicillin resistance (bacteria); uracil resistance (yeast); ligation independent cloning (LIC).
|
PSI
|
EvNO00304417
|
pSGP47
|
Yeast expression vector with Adh2 promoter, adds 3C protease cleavage site and 10xHis tag; ampicillin resistance (bacteria); uracil (yeast); ligation independent cloning (LIC).
|
PSI
|
EvNO00304418
|
pSGP36
|
Yeast expression vector with Gal1 promoter, adds C-terminal 3C-his10 tag; amp resistance (URA for yeast); ligation independent cloning (LIC).
|
PSI
|
EvNO00304419
|
pSGP40
|
Yeast expression vector with Gal1 promoter, adds C-terminal 3C-ZZ-His10 tag; amp resistance (URA for yeast); ligation independent cloning (LIC).
|
PSI
|
EvNO00304420
|
pNYCOMPS-TH-ccdB (C-term.)
|
Bacterial (e. coli) expression vector with T7 promoter, C-terminal 10xHis tag and TEV protease cleavage site; contains ccdB death cassette which is removed after LIC cloning; kanamycin resistance in bacteria; ligation independent cloning.
|
PSI
|
EvNO00305686
|
pMA507
|
E. coli expression vector with a T7 promoter, N-terminal 6xHis tag and TEV protease cleavage site; contains the ccdB death cassette; kanamycin resistance in bacteria; ligation independent cloning (LIC).
|
PSI
|
EvNO00305687
|
pMA510
|
Bacterial expression vector with a T7 promoter, N-terminal MBP fusion-6xHis tag-TEV protease site; contains the ccdB death cassette; kanamycin resistance in bacteria; ligation independent cloning (LIC).
|
PSI
|
EvNO00305688
|
pMAPLe003
|
Bacterial expression vector with a T7 promoter, N-terminal MBP and C-terminal 6xHis tag-TEV protease site; constitutively expresses TEV protease to produce C-terminal His-tagged protein; contains the ccdB death cassette; kanamycin resistance in bacteria; ligation independent cloning (LIC).
|
PSI
|
EvNO00306565
|
pMYC4-H
|
Mycoplasma coexpression expression vector with the Phsp promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning ( NdeI, SbfI).
|
PSI
|
EvNO00306566
|
pMYC5-H
|
Mycoplasma coexpression expression vector with the Phsp promoter and C-termial c-myc tag; modified from pMYC4-H with unique SrfI site; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (NdeI, SbfI).
|
PSI
|
EvNO00306567
|
pMYC4-I
|
Mycoplasma coexpression expression vector with the Pimyc promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PacI, SbfI).
|
PSI
|
EvNO00306568
|
pMYC4-S
|
Mycoplasma coexpression expression vector with the Psmyc promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PacI, SbfI).
|
PSI
|
EvNO00306569
|
pMYC4-Hp
|
Mycoplasma coexpression expression vector with the Phsp promoter and C-termial c-myc tag; newer version of pMYC4-H suitable for universal cloning by PacI and SbfI sites; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PacI, SbfI).
|
PSI
|
EvNO00306570
|
p4-Hb
|
Mycoplasma coexpression expression vector with the Phsp promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (AsiSI, PmeI).
|
PSI
|
EvNO00306571
|
p4-Ib
|
Mycoplasma coexpression expression vector with the Pimyc promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (AsiSI, PmeI).
|
PSI
|
EvNO00306572
|
p4-Sb
|
Mycoplasma coexpression expression vector with the Psmyc promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (AsiSI, PmeI).
|
PSI
|
EvNO00306573
|
p9T-c
|
Mycoplasma coexpression expression vector with the PtetO promoter; tetracycline-inducible expression of insert; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PtetO gene: AsiSI, PmeI; second constitutively expressed gene: PacI, SbfI).
|
PSI
|
EvNO00306585
|
p9T-H
|
Mycoplasma coexpression expression vector with the PtetO promoter and Phsp promoter; tetracycline-inducible expression of insert; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PtetO gene: AsiSI, PmeI; Phsp gene: PacI, SbfI).
|
PSI
|
EvNO00306605
|
p9T-I
|
Mycoplasma coexpression expression vector with the PtetO promoter and Pimyc promoter; tetracycline-inducible expression of insert; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PtetO gene: AsiSI, PmeI; Pimyc gene: PacI, SbfI).
|
PSI
|
EvNO00306625
|
p9T-S
|
Mycoplasma coexpression expression vector with the PtetO promoter and Psmyc promoter; tetracycline-inducible expression of insert; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PtetO gene: AsiSI, PmeI; Psmyc gene: PacI, SbfI).
|
PSI
|
EvNO00306645
|
pVP80K
|
Bacterial expression vector with a T5 promoter and N-terminal 8xHis-MBP tag; kanamycin resistance in bacteria; restriction enzyme cloning.
|
PSI
|
EvNO00306665
|
pVP81K
|
Bacterial expression vector with a T5 promoter with an auto-cleaving (TVMV protease) N-terminal MBP tag that leaves an N-terminal 8xHis tagged protein; kanamycin resistance in bacteria; restriction enzyme cloning.
|
PSI
|
EvNO00306666
|
pVP91A
|
Bacterial expression vector with a T5 promoter and N-terminal 8xHis tag; ampicilliin resistance in bacteria; restriction enzyme cloning.
|
PSI
|
EvNO00306667
|
pEU-HSBC
|
In Vitro SP6 transcription and wheat germ cell free protein expression vector with a SP6 promoter and N-terminal 6xHis tag; ampicillin resistance in bacteria; restriction enzyme cloning.
|
PSI
|
EvNO00306668
|
pVP65K
|
Bacterial (E. coli) expression with N-terminal 8xHis, MBP tag; kanamycin resistance in bacteria; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat cassette.
|
PSI,Promega
|
EvNO00306669
|
pVP68K
|
Bacterial (E. coli) expression with N-terminal 8xHis, MBP tag; kanamycin resistance in bacteria; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat cassette.
|
PSI,Promega
|
EvNO00335423
|
ZM552
|
Yeast expression vector with N-terminal 3X V5 epitope tag; ampicillin resistance in bacteria; LEU2 resistance in yeast; Gateway cloning.
|
|
EvNO00336943
|
pET15_NESG
|
Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.
|
PSI/NESG
|
EvNO00336944
|
pET21_NESG
|
Bacterial (E. coli) expression vector with T7 lac promoter, adds C-terminal 6xHis tag; ampicillin resistance; restriction enzyme cloning.
|
PSI/NESG
|
EvNO00340963
|
pMCSG26
|
Bacterial expression vector with C-terminal 6xHis tag and T7 promoter; ampicillin resistance in bacterial; ligation independent cloning (LIC)
|
PSI
|
EvNO00340964
|
pMCSG28
|
Bacterial expression vector with T7 promoter and TEV-cleavage C-terminal 6xHis; ampicillin resistance in bacterial; ligation independent cloning (LIC)
|
PSI
|
EvNO00340965
|
pMCSG32
|
Bacterial expression vector with T7 promoter and C-terminal 6xHis, TEV-protease cleavage site, N-terminal MBP tag with TVMV site; ampicillin resistance in bacterial; ligation independent cloning (LIC)
|
PSI
|
EvNO00412303
|
pCPD_nGST-SBP_DC
|
Bacterial expression vector with a T7 promoter, N-terminal GST, Thrombin prtease cleavage site, SBP tag, and TEV protease cleavage site; ampicillin and chloamphenicol resistance in bacteria (CmR removed in clones with inserts); Gateway recombinational cloning.
|
|
EvNO00412304
|
pCPD_nMBP-SBP_DC
|
with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, bacterial expression expression with pCPD5698_F and pCPD6268_R and MBP and Thrombin and SBP and TEV and ccdB and CmR and T7 term and LacI and AmpR and ColE and T7 and lac operator and RBS; chloramphenicol resistance in bacterial, ampicillin resistance in bacterial;
|
|
EvNO00413803
|
pCPD_nSBP-Halo_DC
|
Bacterial expression vector with a T7 promoter and N-terminal SBP and Halo tags, seperated by protease cleavage sites for thrombin and TEV protease; ampicillin resistance in bacteria; Gateway cloning
|
HALO
|
EvNO00413804
|
pCPD_nHalo_DC
|
Bacterial expression vector with a T7 promoter and TEV cleavable N-terminal Halo tag; ampicillin resistance in bacteria; Gateway cloning
|
HALO
|
EvNO00424503
|
pJFT7_nHALO_DC
|
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable HALO tag; ampicillin resistance; Gateway or Flexi cloning compatible
|
HALO
|
EvNO00424523
|
pJFT7_cHalo_DC
|
In vitro expression vector with T7 promoter and C-terminal TEV-cleavable Halo tag; ampicillin resistance; Gateway or Flexi cloning compatible
|
HALO
|
EvNO00424543
|
pJFT7_nHalo_empty
|
In vitro expression vector with T7 promoter and N-terminal TEV-cleavable Halo tag; does not contain a death cassette; ampicillin resistance; restriction enzyme cloning
|
HALO
|
EvNO00424544
|
pJFT7_cHalo_empty
|
In vitro expression vector with T7 promoter and C-terminal TEV-cleavable Halo tag; does not contain a death cassette; ampicillin resistance; restriction enzyme cloning
|
HALO
|
EvNO00430383
|
pSGP17
|
Yeast expression vector with a AOX1 promoter C-terminal CBP and RSG-6xHis tag with 3C protease cleavage site and pre-pro-alpha-factor leader sequence; zeocin resistance in Pichia and in bacteria; ligation independent cloning (LIC)
|
PSI
|
EvNO00430384
|
pSGP18
|
Yeast expression vector with a AOX1 promoter C-terminal CBP and RSG-6xHis tag with 3C protease cleavage site; zeocin resistance in Pichia and in bacteria; ligation independent cloning (LIC)
|
PSI
|
EvNO00450863
|
pMCSG53
|
Bacterial expression vector with a T7 promoter and TEV-cleavable N-terminal 6xHis tag; Contains rare argU and ileX tRNA genes; ampicillin resistance in bacteria; ligation independent cloning.
|
PSI
|
EvNO00450864
|
pMCSG68
|
Bacterial expression vector with a T7 promoter and TEV-cleavable N-terminal 6xHis and StrepII tags; rare argU and ileX codons; ampicillin resistance in bacteria; ligation independent cloning
|
PSI
|
EvNO00450865
|
pMCSG69
|
Bacterial expression vector with a T7 promoter and N-terminal MBP tag, TVMV cleavage site, 6xHis tag and TEV protease cleavage site with rare codon argU and ileX tRNA genes; ampicillin resistance in bacteria; ligation independent cloning
|
PSI
|
EvNO00450883
|
pJSP6_nHalo_DC
|
Wheat germ expression vector with a SP6 promoter and N-terminal TEV-cleavable HALO tag; ampicillin resistance in bacteria and chloramphenicol resistance in empty vector; Gateway or Flexi cloning
|
HALO
|
EvNO00450884
|
pJSP6_nHalo_empty
|
Wheat germ expression vector with SP6 promoter and TEV-cleavable N-terminal HALO tag; ampicillin resistance in bacteria; gateway cloning
|
HALO
|
EvNO00454843
|
pMCSG49
|
Bacterial expression vector with a T7 promoter and N-terminal 6xHis tag, TEV cleavable; ampicillin resistance in bacteria; Ligation Independent cloning
|
PSI
|
EvNO00454845
|
pMCSG54
|
Bacterial expression vector with a T7 promoter and N-terminal Flag and 6xHiis tag followed by TEV protease cleavage site ; ampicillin resistance in bacteria; ligation independent cloning.
|
PSI
|
EvNO00454846
|
pMCSG55
|
Bacterial expression vector with T7 promoter and N-terminal 6xHis and HA tags followed by TEV protease cleavage site; ampicillin resistance in bacteria; ligation independent cloning
|
PSI
|
EvNO00454847
|
pMCSG56
|
Bacterial expression vector with T7 promoter and N-terminal 6xHis and double HA tags followed by TEV protease cleavage site; ampicillin resistance in bacteria; ligation independent cloning
|
PSI
|
EvNO00534623
|
pGEn1-DEST
|
Mammalian expression vector with a CMV promoter and N-terminal TCM 8xHis and Strep tag; ampicillin resistance in bacteria; Gateway recombinational cloning
|
SPTA
|
EvNO00534624
|
pGEn2-DEST
|
Mammalian expression vector with a CMV promoter and N-terminal 8xHis, AviTag, and Super GFP tags; ampicillin resistance in bacteria; Gateway recombinational cloning
|
SPTA
|
EvNO00534626
|
pGEc1-DEST
|
Mammalian expression vector with a CMV promoter and C-terminal 8xHis and Strep tags; ampicillin resistance in bacteria; Gateway recombinational cloning
|
SPTA
|
EvNO00534627
|
pGEc2-DEST
|
Mammalian expression vector with a CMV promoter and C-terminal Superfolder GFP, AviTag and 10xHis tag; ampicillin resistance in bacteria; Gateway recombinational cloning
|
SPTA
|
EvNO00548203
|
pET16-DEST
|
Bacterial expression vector with a T7 promoter and a N-terminal 10xHis tag; ampicillin resistance in bacteria; Gateway recombinational cloning.
|
SPTA
|
EvNO00548204
|
pET32-DEST
|
Bacterial expression vector with T7 promoter and N-terminal Trx, 6xHis and S-tags; Thrombin and enterokinase cleavage sites; ampicillin resistance in bacteria; Gateway recombinational cloning
|
SPTA
|
EvNO00587843
|
pJSP6_nGST_DC
|
Wheat germ expression vector with a SP6 promoter and N-terminal TEV-cleavable GST tag; ampicillin resistance; Gateway or Flexi cloning compatible
|
|
EvNO00595403
|
pJFT7_nHIS_cHalo_DC
|
In vitro expression vector with a T7 promoter and N-terminal HIS and C-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
|
HALO
|
EvNO00595404
|
pJFT7_cHalo-3xHA_DC
|
In vitro expression vector with a T7 promoter and C-terminal TEV-cleavable HALO-3xHA tag; ampicillin resistance; Gateway or Flexi cloning compatible
|
HALO
|
EvNO00595405
|
pJFT7_nFlag_cHalo_DC
|
In vitro expression vector with a T7 promoter and N-terminal Flag and C-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
|
HALO
|
EvNO00595406
|
pJFT7_nHA_cHalo_DC
|
In vitro expression vector with a T7 promoter and N-terminal HA and C-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
|
HALO
|
EvNO00595407
|
pJFT7_n3xFlag-Halo_DC
|
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable 3xFlag-Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
|
HALO
|
EvNO00595408
|
pJFT7_cHalo-3xFlag_DC
|
In vitro expression vector with a T7 promoter and C-terminal TEV-cleavable HALO-3xFlag tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
|
HALO
|
EvNO00595409
|
pJFT7_n3xHA-Halo_DC
|
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable 3xHA-Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
|
HALO
|
EvNO00599463
|
pMCSG58
|
Bacterial expression vector with T7 promoter and C-terminal 6xHis tag; contain argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning
|
PSI
|
EvNO00599464
|
pMCSG59
|
Bacterial expression vector with T7 promoter and TEV-cleavable C-terminal 6xHis tag; contain argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning
|
PSI
|
EvNO00599484
|
pMCSG63
|
Bacterial co-expresion vector with T7 promoter and N-terminal TEV-cleavable 6xHis tag; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning
|
PSI
|
EvNO00599485
|
pMCSG70
|
Bacterial expression vector with T7 promoter and C-terminal TEV-cleavable 10xHis tag and TVMV-cleavable MBP tag; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning
|
PSI
|
EvNO00599486
|
pMCSG71
|
Bacterial expression vector with T7 promoter, N-terminal TVMV-cleavable MBP tag and N-terminal TEV-cleavable 6xHis tag; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning
|
PSI
|
EvNO00599503
|
pMCSG75
|
Bacterial expression vector with T7 promoter and TEV-cleavable 6xHis tag BirA gene; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning
|
PSI
|
EvNO00599504
|
pMCSG76
|
Bacterial co-expression vector with T7 promoter and N-terminal TEV-cleavable 6xHis tag; contains argU and ileX rare tRNA; spectinomycin resistance in bacteria; ligation independent cloning
|
PSI
|
EvNO00599523
|
pMCSG77
|
Bacterial co-expression vector with T7 promoter and N-terminal TEV-cleavable 6xHis tag; contains argU and ileX rare tRNA; kanamycin resistance in bacteria; ligation independent cloning
|
PSI
|
EvNO00601503
|
pLIFE-3'UTR
|
Mammalian expression vector for detecting the expression of 3'UTRs by Firefly luciferase; also contains Renilla luciferase for normalization; ampicillin resistance in bacteria; Gateway cloning with P2RP3 Gateway Cassette
|
SPTA
|
EvNO00601504
|
pLIFE-miRNA
|
Mammalian expression vector for expressing miRNAs; also contains red fluorescent protein, gateway cassette located within an intron of RFP 3'UTR, for ncRNA expression; ampicillin resistance in bacteria; Gateway cloning with L2R3 Gateway Cassette
|
SPTA
|
EvNO00601603
|
pCMV(delta1)
|
Mammalian expression vector with a deletion mutant of the CMV promoter; ampicillin resistance in bacteria, neomycin resistance in mammalian cells; restriction enzyme cloning
|
SPTA
|
EvNO00601604
|
pCMV(delta2)
|
Mammalian expression vector with a deletion mutant of the CMV promoter; ampicillin resistance in bacteria, neomycin resistance in mammalian cells; restriction enzyme cloning
|
SPTA
|
EvNO00601605
|
pCMV(delta3)
|
Mammalian expression vector with a deletion mutant of the CMV promoter; ampicillin resistance in bacteria, neomycin resistance in mammalian cells; restriction enzyme cloning
|
SPTA
|
EvNO00601606
|
pCMV(delta4)
|
Mammalian expression vector with a deletion mutant of the CMV promoter; ampicillin resistance in bacteria, neomycin resistance in mammalian cells; restriction enzyme cloning
|
SPTA
|
EvNO00601607
|
pCMV(delta5)
|
Mammalian expression vector with a deletion mutant of the CMV promoter; ampicillin resistance in bacteria, neomycin resistance in mammalian cells; restriction enzyme cloning
|
SPTA
|
EvNO00601608
|
pCMV(delta6)
|
Mammalian expression vector with a deletion mutant of the CMV promoter; ampicillin resistance in bacteria, neomycin resistance in mammalian cells; restriction enzyme cloning
|
SPTA
|
EvNO00601609
|
pCMV(WT)
|
Mammalian expression vector with a CMV promoter; ampicillin resistance in bacteria, neomycin resisatance in mammalian cells; restriction enzyme cloning
|
SPTA
|
EvNO00621023
|
pCPD_nHalo_cHIS_DC
|
Bacterial expression with a T7 promoter, N-terminal TEV-cleavable HaloTag and C-terminal 3C protease cleavable 6xHis tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
|
HALO
|
EvNO00621024
|
pJFT7_nHalo_DC(r3)
|
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
|
HALO
|
EvNO00623103
|
pJFT7_nGST_DC-r2
|
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
|
|
EvNO00623703
|
pRSET-natGFP
|
Bacterial expression vector with a T7 promoter; adds C-terminal GFP tag; ampicillin resistance in bacteria; ligation independent cloning
|
PSI
|
EvNO00623704
|
pRSET-natGFPHis
|
Bacterial expression vector with a T7 promoter; adds C-terminal GFP-His6 tag; ampicillin resistance in bacteria; ligation independent cloning
|
PSI
|
EvNO00623723
|
pJFT7_nHalo_DC(r4)
|
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
|
HALO
|
EvNO00623724
|
pJSP6_cHalo_DC
|
Wheat germ expression vector with SP6 promoter and TEV-cleavable C-terminal HALO tag; ampicillin resistance in bacteria; gateway cloning
|
HALO
|
EvNO00629003
|
pET-32b-TEV
|
Bacterial expression vector with T7 promoter and N-terminal E. coli thioredoxin tag, 6xHis-tag, thrombin cleavage site, S-tag, and TEV cleavage site; ampicillin resistance in bacteria; restriction enzyme cloning
|
PSI
|
EvNO00629004
|
pRSET-natTEVGFPHis
|
Bacterial expression vector with T7 promoter and C-terminal TEV-cleavavable GFP and 6xHis-tags; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
|
PSI
|
EvNO00629005
|
pCMVi-LSrCOMPTT
|
with tag/fusion/marker assay, ligation independent cloning (LIC) clone, mammalian expression expression with intron and CMV and oligomerization domain and hGH and SV40 and leader sequence and AmpR and f1 and ColE and peptide and -10/Spec-R and COMP and Tetanus Toxoid and epitope and Stop Codons and InTron For and 013_DO3_DTH21 and RBS and 014_C03_DTH22 and 2012 and HGHTER and CMV-F; ampicillin resistance in bacterial;
|
PSI
|
EvNO00629006
|
pCMVi-UB
|
Vector for antibody production in mice upon genetic immunization by gene gun with CMV promoter and N-terminal mouse ubiqiutin protein with Gly76Ala mutation; ampicillin resistance in bacteria; restriction enzyme cloning
|
PSI
|
EvNO00629007
|
pBAD24sigNtermHis
|
Arabinose-inducible bacterial expression with a pBAD promoter and N-terminal E. coli YtfM (aa1-21) and 6xHis-tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
|
PSI
|
EvNO00629008
|
pBAD24sigCtermHis
|
Arabinose-inducible expression in bacteria with N-terminal E. coli YtfM (aa1-21) signal sequence and C-terminal His-tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
|
PSI
|
EvNO00629009
|
pRSET-YtfM-8xHis-N-term
|
Bacterial expression with T7 promoter and N-terminal E. coli YtfM (aa1-21) signal sequence and 8xHis-tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
|
PSI
|
EvNO00629010
|
pRSET-C8xHis
|
Bacterial expression vector with T7 promoter and C-terminal 8xHis tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
|
PSI
|
EvNO00629011
|
pRSET-C6xHis
|
Bacterial expression vector with T7 promoter and C-terminal 6xHis tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
|
PSI
|
EvNO00631283
|
pCMVi-LS
|
with tag/fusion/marker assay, multiple cloning site clone, bacterial expression expression with leader sequence and MCS and intron and hGH and forward primer and AmpR and ColE and f1 and reverse primer and DTH and -10/Spec-R and Stop Codons and CMV and SV40; ampicillin resistance in bacterial;
|
PSI
|
EvNO00631284
|
pRSET-YtfM-AN
|
Bacterial expression vector with T7 promoter and N-terminal E. coli YtfM (aa1-23) and 7xHis-tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
|
PSI
|
EvNO00631285
|
pCDF-BAD
|
Arabinose-inducible expression in bacteria; spectinomycin resistance in bacteria; ligation into multiple cloning site downstream of RBS: NdeI, XhoI/SciI, AscI, ZraI/AatII; or just upstream of RBS: EagI/NotI, PmeI
|
PSI
|
EvNO00665463
|
pANT7_GST-control
|
In vitro expression vector expressing two GSTs as a control vector for clones in pANT7_cGST; ampicillin resistance in bacteria; recombinational cloning.
|
|
EvNO00813763
|
pPPL
|
ligation independent cloning (LIC) clone with pBR322 origin and Ampicillin Resistance and ROP and ORF frame 2 – Tetracycline resistance and ORF frame 1 and pBRrevBam and T7 and T7 term primer and BseRI Stuffer and MBP signal sequence (M1-A26 of NP_290668) and RBS and MBP (K27-T392 of NP_290668) and HIS10 and TEV and FC sequencing primer pET26 pelB seq R and FC sequencing primer MBP pelB seqF and FC sequencing primer pET-PPL seq 1 F and FC sequencing primer pET-PPL seq 1 R and FC sequencing primer pET-PPL seq 2 F and T7 term and FC sequencing primer pET-PPL seq 2 R and AmpR; ampicillin resistance in bacterial;
|
|
EvNO00813764
|
pET-BAD
|
ligation independent cloning (LIC) clone, bacterial expression expression with Operator O2 and araC promoter and T7 term and Operator O1 and B-lactamase gene and CAP site and T7 term primer and pBR322 origin and Operator I1 and I2 and arabinose BAD promoter and rrnB T1 T2 and RBS and ARAC3F Primer and PCBSREV Primer and ORICOL Primer and BADT1A Primer and BADT1B Primer and AMP5R Primer and -35 AP and F1ORI Primer and ARAC5R and -10 AP and rep_origin and ROP and araC; ampicillin resistance in bacterial;
|
|
EvNO00813765
|
pRSF-BAD
|
ligation independent cloning (LIC) clone, bacterial expression expression with Kan-R and T7TER primer (#35) and T7 Terminator and RNA II and RNA I and RSF ori - RSF1030 Homology and -10 RNA I and -35 RNA I and -35 AP and -10 AP and RBS-reverse-weak and araC and Operator O2 and araC promoter and Operator O1 and CAP site and Operator I1 and I2 and arabinose BAD promoter and rrnB T1 T2 and RBS and ligation junction Xbal and BADT1A Primer and BADT1B Primer and ARAC3F primer (#145) and ARAC5R primer (#148) and wrong primer #62 off by 3 nt and KAN1F primer (#120) and ligation junction Pacl; 50ug/mL of Kanamycin resistance in bacterial;
|
|
EvNO00813783
|
pelB-MBP
|
ligation independent cloning (LIC) clone, bacterial expression expression with RBS (extended) and TET-2 and pelB and pBR322 origin and KanR and ROP and BseRI and HIS10 and MBP and TEV and FC sequencing primer pET26 pelB seq R and FC sequencing primer MBP pelB seqF and TET and LacI and FC sequencing primer pET-PPL seq 1 F and FC sequencing primer pET-PPL seq 1 R and FC sequencing primer pET-PPL seq 2 F and pBRrevBam and T7 and lac operator and T7 term primer; kanamycin resistance in bacterial;
|
|
EvNO00813803
|
pCPD-pRL-CMV-ccdb
|
fluorescent marker assay, Gateway cloning, bacterial expression expression with Renilla Luciferase and pCEP Fwd Primer and ccdB and CMV and CAGF and CMV-F and Amp-R and lac promoter and attR1 and attR2 and T7 promoter and Chloramphenicol-R; chloramphenicol resistance in bacterial, ampicillin resistance in bacterial;
|
|
EvNO00846203
|
pPSU1
|
High copy number plasmid which provides 500, 1000, 1500, 2000 and 5000 bp EcoRV fragments of kilobase DNA ladder, and 500, 700, 800, 900, 1000, 2000 and 4100 bp PstI fragments of 100 bp DNA ladder. Confers ampicillin resistance.
|
|
EvNO00846223
|
pPSU2
|
High copy number plasmid which provides 750, 3000 and 4000 bp EcoRV fragments of kilobase DNA ladder, and 50, 100, 200, 300, 400, 500, 600, 1500 and 4100 bp PstI fragments of 100 bp DNA ladder. Confers ampicillin resistance.
|
|
EvNO00877063
|
pLX304
|
with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, lentiviral infection expression with BlastR and T7 and attR1 and CMV and cPPT and V5 and AmpR and attR2 and CAT and ccdB and WPRE; ampicillin resistance in bacterial, blasticidin resistance in mammalian;
|
|
EvNO00877064
|
pLV_R4R2_GTW3
|
with tag/fusion/marker assay, Gateway® clone, retroviral vector expression with RRE; chloramphenicol resistance in bacterial, ampicillin resistance in bacterial, blasticidin resistance in mammalian;
|
|
EvNO00877066
|
pMK-Dest
|
with tag/fusion/marker assay, Gateway® clone, bacterial expression expression with CMV and CMV-F and attR1 and attR2 and lac promoter and pBR322 origin and NeoR and AttB2 and TEV; kanamycin resistance in bacterial, neomycin resistance in bacterial;
|
|