Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
FLH464757.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
ZM552
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
ScCD00100602
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5904nts
Open reading frame: 1 to 5904
ATGTCAACTGAATACTCTGCTGATTCATCTAAATCATTTATGATTGCAATGCAATCTATG
ATTGATACTTCACAAACCTTTAATCTAGATAGATCGAAAATCTCTTTACCAGATTTTGAT
GACGAGCTAAAAAAAGTCCAAAAGGATGAACAAAATCAAAGAACTGAACTAACTGTTCTC
TCACAAGATAGAAATGATTGGGATGATATATTTGAGGAGTTTAAAGACATTTCATTTGCT
CAACTGCAATCTATAATTGATTCATACAAAACAAAGAATGCAGTTGCAGTATATAAAAAA
ATTGGAAAACTAATCAATGAAGCTGAAACGACACTAAGCAGCAATGTATTATTAGAAACT
GTACTGCAAATGGTTTATAAACATCAAAAGCAAGAGTTAGAGAAAGAATTGTTGGATTTT
TTAGGAACAGGGAATATAGACCTTGTTTCTCTGTTACTTCAGCATAGAAGAATGATCGTT
GCTACACCTATAGAAACGACGATTCTGCTAATAAAAAATGCGGTCAATTCAACGCCAGAG
TTCCTGACACAGCAAGATATCAGGAATCAAGTTTTGAAAAGTGCAGAGGATGCCAAGAAT
AGGAAATTAAATCCCGCCACTAAAATAATCAAGTACCCACACGTCTTCAGAAAATATGAA
GCAGGATCCACTACAGCTATGGCCTTTGCCGGTCAAAAATTCACTTTGCCAGTAGGGACT
ACAAGGATGTCGTACAATACCCATGAAGAAATTATAATTCCGGCAGCAGATCAGGCATCC
AACAAGAACTATCTCTATACAAAACTATTGAAAATAAGTGATTTGGATCATTTTTGCAAG
ACTGTATTTCCCTATGAAACTTTAAACCAAATACAATCCCTTGTTTACCCCGTAGCTTAT
AAAACAAATGAAAATATGCTAATTTGTGCACCTACTGGTGCAGGTAAAACAGACATTGCA
TTACTGACAATAATTAATACAATAAAACAATTCTCCGTGGTCAACGGGGAAAATGAAATT
GATATTCAATATGATGATTTTAAAGTTATTTATGTTGCCCCTTTGAAAGCACTCGCTGCT
GAAATCGTCGACAAGTTTAGTAAGAAATTGGCGCCTTTTAATATACAAGTAAGGGAGTTA
ACAGGCGATATGCAATTGACAAAGGCGGAAATTTTAGCCACTCAAGTAATAGTCACAACC
CCTGAGAAATGGGACGTTGTCACCCGTAAAGCAAACGGCGACAACGATTTGGTTTCAAAG
GTCAAATTACTAATTATTGATGAAGTTCATTTACTGCACGAAGATAGAGGTTCGGTTATT
GAAACTTTAGTTGCACGTACCTTGAGACAGGTAGAAAGCTCTCAATCAATGATTCGTATT
ATCGGTCTATCAGCTACATTACCCAATTTTATGGATGTAGCAGATTTCTTAGGAGTAAAT
AGACAGATCGGAATGTTCTATTTTGATCAATCATTCCGTCCAAAGCCTCTAGAGCAGCAA
CTTTTGGGCTGTAGAGGTAAGGCAGGTAGTAGACAAAGTAAAGAGAATATCGATAAAGTC
GCTTACGACAAATTGAGTGAAATGATTCAAAGAGGGTACCAGGTAATGGTATTTGTCCAC
TCAAGAAAGGAAACTGTTAAAAGTGCAAGAAACTTTATCAAACTAGCAGAATCTAACCAC
GAAGTTGACCTTTTTGCACCTGACCCTATCGAAAAGGATAAATATTCTAGAAGTTTAGTG
AAGAACAGAGACAAGGATATGAAGGAGATTTTTCAATTTGGTTTTGGTATCCATCATGCT
GGTATGGCCCGTTCAGATAGAAATCTTACTGAAAAAATGTTTAAAGACGGTGCTATCAAG
GTACTGTGTTGTACTGCAACATTAGCATGGGGTGTTAATTTACCTGCTGATTGTGTTATC
ATCAAGGGTACACAAGTATATGATTCTAAAAAGGGTGGATTCATAGATCTAGGTATTTCA
GATGTTATTCAGATTTTTGGCAGGGGTGGTAGGCCGGGCTTCGGTTCCGCAAATGGTACG
GGTATTTTATGTACTTCCAATGATCGTCTGGATCATTATGTTTCCTTAATTACTCAACAA
CACCCTATCGAATCTAGATTCGGTTCCAAGTTGGTTGATAACCTGAACGCCGAAATTTCA
TTGGGGAGCGTGACAAATGTAGATGAAGCAATCGAATGGCTTGGTTACACATACATGTTT
GTTCGTATGAGAAAAAATCCTTTTACTTACGGTATTGATTGGGAGGAAATTGCAAATGAC
CCGCAATTATATGAACGGAGGAGAAAAATGATCGTAGTAGCTGCAAGGCGATTACATGCT
CTCCAAATGATTGTGTTCGATGAAGTGTCAATGCATTTCATTGCAAAGGATTTGGGCAGG
GTGTCCTCTGATTTTTATCTTTTGAACGAATCCGTCGAGATATTCAATCAAATGTGTGAT
CCCAGAGCCACCGAAGCTGATGTATTGTCCATGATCAGTATGAGCAGTGAATTTGACGGT
ATAAAATTTAGAGAAGAAGAATCCAAGGAGTTGAAGAGACTTTCTGATGAATCTGTTGAG
TGCCAGATTGGAAGCCAACTTGACACCCCTCAAGGTAAAGCAAACGTGCTATTACAGGCT
TATATTTCCCAGACTAGAATTTTTGACTCAGCACTATCATCCGATTCGAATTATGTTGCT
CAGAACTCTGTTAGAATTTGTAGAGCATTGTTTTTAATAGGTGTCAATAGAAGATGGGGT
AAATTTTCAAACGTCATGCTAAATATTTGCAAATCTATTGAAAAGAGGCTCTGGGCCTTT
GATCACCCTCTCTGTCAGTTTGATTTGCCTGAAAATATTATCAGACGCATCAGAGATACT
AAACCTTCCATGGAGCATCTTTTAGAATTAGAGGCAGATGAGCTCGGAGAGCTGGTGCAT
AATAAGAAGGCCGGGTCTAGGTTATATAAAATATTGAGCCGATTTCCAAAGATTAATATA
GAAGCTGAGATTTTCCCCATTACAACAAATGTAATGAGGATACATATTGCATTAGGTCCA
GACTTTGTATGGGATTCCCGGATACATGGTGATGCACAGTTTTTTTGGGTTTTCGTTGAA
GAATCTGATAAGTCGCAAATCCTTCATTTTGAAAAGTTCATCTTAAACAGAAGGCAGTTG
AATAATCAGCATGAAATGGACTTCATGATCCCATTGTCAGACCCTTTGCCTCCGCAGGTT
GTCGTAAAGGTTGTGTCGGATACTTGGATTGGCTGTGAATCGACCCATGCCATCTCTTTT
CAACATTTAATCAGACCATTTAACGAAACTTTACAGACAAAATTGTTGAAGTTACGTCCT
CTACCTACATCAGCCCTCCAGAATCCTTTAATTGAGTCAATTTATCCTTTCAAGTATTTC
AATCCCATGCAAACTATGACATTTTATACACTTTACAATACCAATGAGAATGCGTTTGTT
GGCTCGCCTACAGGTTCTGGTAAAACGATTGTGGCTGAATTGGCTATATGGCATGCCTTC
AAAACATTTCCTGGGAAAAAGATTGTTTATATCGCACCAATGAAAGCATTAGTACGTGAA
AGGGTTGATGACTGGAGAAAGAAAATAACACCAGTGACCGGAGATAAAGTTGTAGAGTTA
ACCGGTGATTCTTTGCCAGATCCTAAAGATGTGCATGATGCCACCATTGTTATTACAACT
CCAGAAAAGTTTGATGGTATTTCCCGTAATTGGCAAACTCGTAAGTTTGTTCAAGATGTA
TCTTTAATTATCATGGATGAAATTCATTTGTTGGCTAGTGATCGTGGTCCTATCTTAGAA
ATGATTGTTAGTCGTATGAATTATATTTCCTCTCAAACCAAGCAACCGGTCAGACTGCTA
GGTATGTCAACTGCTGTTTCAAACGCCTACGACATGGCTGGCTGGTTGGGAGTTAAGGAC
CACGGACTGTATAACTTCCCGTCAAGCGTCCGTCCGGTTCCATTGAAAATGTACATTGAT
GGATTTCCAGATAATTTGGCATTCTGTCCTCTGATGAAAACAATGAACAAACCCGTGTTT
ATGGCCATTAAACAACATTCACCTGATAAACCAGCATTAATATTTGTTGCATCGCGTAGA
CAAACTAGACTAACGGCTTTAGACTTGATCCACTTATGTGGAATGGAAGATAATCCACGT
AGGTTTTTGAATATAGACGATGAGGAGGAACTGCAATATTACTTATCACAGGTCACTGAC
GACACTTTAAAGTTATCACTCCAGTTCGGTATTGGATTGCATCATGCAGGTCTAGTGCAA
AAAGATCGTTCTATTTCTCATCAGTTGTTTCAAAAAAACAAAATTCAGATTCTGATCGCT
ACATCAACCCTAGCGTGGGGTGTCAATTTGCCAGCTCACTTGGTTATCATCAAAGGTACA
CAATTTTTTGATGCAAAAATTGAAGGATATAGAGATATGGATTTAACAGATATCTTGCAA
ATGATGGGGAGAGCTGGTAGACCGGCTTATGACACTACAGGTACTGCTATCGTTTATACA
AAAGAGTCCAAAAAAATGTTTTATAAACATTTCCTAAACGTTGGTTTTCCTGTCGAGTCC
TCCTTGCACAAGGTATTAGATGATCATTTAGGTGCAGAAATAACGTCAGGTAGCATAACT
AATAAGCAAGAAGCGTTAGATTTTTTGAGTTGGACATTTTTGTTTCGTAGAGCGCATCAT
AACCCGACATATTATGGCATTGAAGATGATACAAGTACAGCAGGAGTCAGTGAACATTTG
AGTTCTTTGATAGATAGCACATTAGAAAATCTGCGAGAATCACAATGTGTTTTATTACAT
GGAGATGATATTGTTGCTACGCCATTCTTAAGTATATCCTCATACTACTATATTTCACAT
TTAACGATACGCCAATTATTGAAACAGATACATGATCATGCGACTTTTCAAGAGGTATTA
CGATGGTTATCGTTGGCTGTTGAGTATAATGAATTGCCTGTAAGAGGTGGTGAAATTATC
ATGAATGAAGAAATGTCGCAGCAATCGAGGTACTCCGTTGAAAGCACTTTTACCGATGAA
TTCGAATTACCGATGTGGGATCCACATGTAAAAACATTTTTATTACTTCAAGCTCATTTA
AGCCGTGTAGATTTACCGATTGCAGATTATATCCAGGATACCGTATCAGTTTTGGATCAA
TCTCTACGTATTCTACAGGCTTATATTGATGTCGCCAGTGAATTGGGTTACTTTCATACG
GTATTGACCATGATAAAGATGATGCAATGCATAAAGCAAGGGTATTGGTATGAAGACGAT
CCTGTTAGTGTACTTCCAGGATTACAACTGAGAAGAATCAAAGATTACACTTTTAGCGAG
CAGGGGTTCATTGAAATGACGCCGCAGCAAAAGAAGAAAAAGCTGTTAACCTTGGAGGAA
ATAGGAAGGTTTGGATATAAGAAATTATTAAATGTTTTTGATCAACTAACTTTTGGAATG
ACAGAAAGCGAAGATACGAAAAAACGTTTCGTCAGTGTCTGCCAAAGATTACCGGTTTTA
GAAGGCATGAAATTTGAAGAACAGGAAAATAACGAGGTGTTAACATTTTATAGCAAGCAC
CTTTCTAGTAAGCATAATAATGGGTTTGAAGTATATTGTGATAAATTCCCTAAGATACAA
AAAGAACTATGGTTTTTAATTGGACACAAAGGCGATGAACTGCTGATGATTAAAAGGTGT
CAACCAAAACAAATGAATAAAGAGGTCATAATACATTGTGATCTATTTATTCCCGAAGAA
ATTCGTGGCGAAGAATTGCAATTTTCCCTTATTAATGACGCTTTAGGATTACGTTATGAT
ATGGTCCATAAACTAATCTCTTAG
Protein sequence predicted based on nucleotide sequence
MSTEYSADSSKSFMIAMQSMIDTSQTFNLDRSKISLPDFDDELKKVQKDEQNQRTELTVL SQDRNDWDDIFEEFKDISFAQLQSIIDSYKTKNAVAVYKKIGKLINEAETTLSSNVLLET VLQMVYKHQKQELEKELLDFLGTGNIDLVSLLLQHRRMIVATPIETTILLIKNAVNSTPE FLTQQDIRNQVLKSAEDAKNRKLNPATKIIKYPHVFRKYEAGSTTAMAFAGQKFTLPVGT TRMSYNTHEEIIIPAADQASNKNYLYTKLLKISDLDHFCKTVFPYETLNQIQSLVYPVAY KTNENMLICAPTGAGKTDIALLTIINTIKQFSVVNGENEIDIQYDDFKVIYVAPLKALAA EIVDKFSKKLAPFNIQVRELTGDMQLTKAEILATQVIVTTPEKWDVVTRKANGDNDLVSK VKLLIIDEVHLLHEDRGSVIETLVARTLRQVESSQSMIRIIGLSATLPNFMDVADFLGVN RQIGMFYFDQSFRPKPLEQQLLGCRGKAGSRQSKENIDKVAYDKLSEMIQRGYQVMVFVH SRKETVKSARNFIKLAESNHEVDLFAPDPIEKDKYSRSLVKNRDKDMKEIFQFGFGIHHA GMARSDRNLTEKMFKDGAIKVLCCTATLAWGVNLPADCVIIKGTQVYDSKKGGFIDLGIS DVIQIFGRGGRPGFGSANGTGILCTSNDRLDHYVSLITQQHPIESRFGSKLVDNLNAEIS LGSVTNVDEAIEWLGYTYMFVRMRKNPFTYGIDWEEIANDPQLYERRRKMIVVAARRLHA LQMIVFDEVSMHFIAKDLGRVSSDFYLLNESVEIFNQMCDPRATEADVLSMISMSSEFDG IKFREEESKELKRLSDESVECQIGSQLDTPQGKANVLLQAYISQTRIFDSALSSDSNYVA QNSVRICRALFLIGVNRRWGKFSNVMLNICKSIEKRLWAFDHPLCQFDLPENIIRRIRDT KPSMEHLLELEADELGELVHNKKAGSRLYKILSRFPKINIEAEIFPITTNVMRIHIALGP DFVWDSRIHGDAQFFWVFVEESDKSQILHFEKFILNRRQLNNQHEMDFMIPLSDPLPPQV VVKVVSDTWIGCESTHAISFQHLIRPFNETLQTKLLKLRPLPTSALQNPLIESIYPFKYF NPMQTMTFYTLYNTNENAFVGSPTGSGKTIVAELAIWHAFKTFPGKKIVYIAPMKALVRE RVDDWRKKITPVTGDKVVELTGDSLPDPKDVHDATIVITTPEKFDGISRNWQTRKFVQDV SLIIMDEIHLLASDRGPILEMIVSRMNYISSQTKQPVRLLGMSTAVSNAYDMAGWLGVKD HGLYNFPSSVRPVPLKMYIDGFPDNLAFCPLMKTMNKPVFMAIKQHSPDKPALIFVASRR QTRLTALDLIHLCGMEDNPRRFLNIDDEEELQYYLSQVTDDTLKLSLQFGIGLHHAGLVQ KDRSISHQLFQKNKIQILIATSTLAWGVNLPAHLVIIKGTQFFDAKIEGYRDMDLTDILQ MMGRAGRPAYDTTGTAIVYTKESKKMFYKHFLNVGFPVESSLHKVLDDHLGAEITSGSIT NKQEALDFLSWTFLFRRAHHNPTYYGIEDDTSTAGVSEHLSSLIDSTLENLRESQCVLLH GDDIVATPFLSISSYYYISHLTIRQLLKQIHDHATFQEVLRWLSLAVEYNELPVRGGEII MNEEMSQQSRYSVESTFTDEFELPMWDPHVKTFLLLQAHLSRVDLPIADYIQDTVSVLDQ SLRILQAYIDVASELGYFHTVLTMIKMMQCIKQGYWYEDDPVSVLPGLQLRRIKDYTFSE QGFIEMTPQQKKKKLLTLEEIGRFGYKKLLNVFDQLTFGMTESEDTKKRFVSVCQRLPVL EGMKFEEQENNEVLTFYSKHLSSKHNNGFEVYCDKFPKIQKELWFLIGHKGDELLMIKRC QPKQMNKEVIIHCDLFIPEEIRGEELQFSLINDALGLRYDMVHKLIS*
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
5904
Mutations:
None
Discrepancy:
c152a,P51Q; g577a,E193K;
c1312t,P438S; t4074c
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
yeast
Marker:
LEU2
Host Type: bacterial Marker: ampicillin
Host Type: bacterial Marker: ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
ZM552
Synonyms:
ZM552
Description:
Yeast expression vector with N-terminal 3X V5 epitope tag; ampicillin resistance in bacteria; LEU2 resistance in yeast; Gateway cloning.
Comments:
ZM511 Stu1 vector plus Gateway attR frame B cassette EcoRV fragment; same as ZM515. Cloning into the vector removes the ccdB cassette and chloramphenicol resistance.
Size (bp):
8302
Parent Vector:
None
Properties:
Gateway, acceptor (destination), recombinational cloning, with tag/fusion/marker, yeast expression
Author Name:
Zarmik Moqtaderi
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
primer site
M13F
M13 forward sequencing primer
8286
8301
primer site
M13R
M13 reverse sequencing primer
2630
2647
promoter
CMD1
CMD1 promoter
295
484
recombination site
attR1
attR1 recombinational cloning site
650
666
recombination site
attR2
attR2 recombination site
2337
2352
reporter gene
LacZ
LacZ alpha gene
8146
8214
selectable marker
AmpR
ampcillin resistance
3795
4454
selectable marker
CmR
chloramphenicol resistance. removed with the ccdB cassette in clones with insert
883
1539
selectable marker
LEU2
LEU2 selectable marker in yeast
5273
5344
tag
V5
N-terminal triple V5 tag
494
631
trxn termination sequence
CYC1
CYC1 terminator
2377
2582
yeast ARS
ARS
ARS yeast origin of replication
0
0
yeast centromere
CEN
yeast centromere
0
0
