ELP2 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH112422.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
ScCD00010113
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2367nts
Open reading frame: 1 to 2367

ATGGTGGAATGTATCACTCCCGAAGCCATTTTTATAGGTGCTAACAAGCAAACTCAGGTT
AGCGATATACACAAAGTTAAAAAAATCGTCGCATTTGGCGCAGGTAAAACTATTGCATTA
TGGGACCCCATTGAACCAAATAATAAAGGTGTTTATGCAACTTTGAAAGGCCACGAAGCT
GAAGTGACTTGTGTCAGATTTGTCCCAGATTCAGATTTTATGGTATCTGCATCTGAGGAT
CATCATGTTAAGATCTGGAAATTTACTGATTACTCACATTTACAATGTATTCAAACCATT
CAACATTACTCGAAGACGATTGTGGCATTAAGCGCTTTACCAAGTCTTATTTCAGTCGGT
TGTGCTGATGGTACTATCAGCATATGGAGACAAAATATTCAAAATGATGAGTTTGGTCTT
GCTCATGAATTCACCATAAAAAAGGGATTCTTCTATCCACTGTGTTTATCACTGTCAAAA
GTTGAAGAAAAAAAGTATTTACTTGCCATTGGGGGTACTAATGTGAACGTTTTTATCGCA
TCTTTCATTTTAAGTGATTCTGGCATTGAGAAGTGTCGAGTGGTTGCAGAATTGGAAGGT
CATGAAGACTGGGTCAAGTCATTGGCTTTCCGTCACCAGGAAACTCCGGGTGATTATTTG
TTATGCTCTGGCTCTCAGGACCGTTATATTAGATTGTGGAGAATTAGGATTAACGATTTA
ATCGATGACTCTGAAGAAGATTCAAAGAAATTAACATTATTGAGCAATAAACAATATAAA
TTTCAAATTGATGATGAATTGAGAGTAGGCATAAATTTTGAAGCCTTAATTATGGGCCAT
GATGATTGGATTTCATCCCTTCAGTGGCACGAAAGTCGCTTACAACTACTAGCTGCTACT
GCTGATACTTCATTGATGGTATGGGAACCTGATGAGACTTCAGGCATTTGGGTGTGCAGT
CTACGGTTAGGAGAAATGTCCTCAAAAGGTGCCTCAACTGCAACGGGTTCCTCTGGAGGT
TTTTGGTCCTGTCTATGGTTTACTCATGAGAGAATGGATTTCTTTTTGACCAATGGTAAG
ACTGGGTCCTGGAGGATGTGGGCTACAAAGGATAATATTATTTGCGATCAAAGGTTGGGC
ATATCCGGTGCCACGAAGGATGTGACGGACATTGCTTGGTCTCCTAGCGGCGAGTACCTA
TTGGCTACTTCTCTGGATCAGACCACTAGACTCTTTGCTCCATGGATTTATGATGCCAGC
GGGAGAAAAAGGGAAATAGCTACCTGGCATGAATTTTCTAGGCCACAAATACATGGTTAT
GATATGATTTGTGTTGAGACAGTGACGGATACCAGATTTGTAAGTGGTGGTGATGAAAAA
ATCTTGAGATCATTTGACCTACCTAAAGGGGTGGCAGGAATGTTACAAAAGTTTGTTGGT
ATTCAATTTGAGGAAAAAAGTGAAATGCCAGACTCTGCTACCGTTCCTGTGTTAGGTTTG
TCTAATAAAGCTGGTGAGGATGACGCAAATGAAGATGATGAAGAGGAGGAAGGTGGTAAT
AAAGAAACACCAGATATCACTGATCCATTGTCCTTGCTAGAATGTCCACCTATGGAAGAC
CAATTGCAAAGACACTTGTTATGGCCTGAAGTGGAAAAGCTTTACGGACATGGTTTTGAA
ATAACGTGTCTTGATATTTCTCCGGATCAGAAATTAATTGCTTCTGCTTGTAGACCAAAT
AATGTTCAGAATGCAGTTATTAGAATATTCAGCACAGAAAATTGGTTAGAAATAAAGCCT
GCATTACCGTTCCACAGTCTAACGATAACAAGATTAAAATTCTCTAAAGATGGAAAATTT
TTATTGAGTGTTTGTAGAGACAGAAAATGGGCGCTTTGGGAAAGAAATATGGAAGATAAT
ACTTTTGAATTGAGATTCAAAAATGAAAAACCACACACAAGGATTATATGGGATGCAGAC
TGGGCACCATTAGAGTTTGGTAATGTTTTTGTCACCGCGTCCAGGGATAAAACTGTCAAA
GTATGGAGGCACCAAAAGGAGCCAGCTGATGACTACGTTCTGGAAGCATCAATTAAACAC
ACTAAAGCTGTCACTGCTATTTCCATACATGACAGCATGATAAGAGAGAAAATTTTGATA
TCGGTGGGTCTTGAAAACGGTGAGATTTATTTATACAGTTATACTTTGGGAAAGTTTGAG
TTAATAACGCAGCTAAACGAGGATATAACACCAGCAGACAAAATTACAAGATTGAGGTGG
TCACATTTAAAGAGAAACGGTAAATTATTTTTAGGTGTAGGAAGTAGTGATTTGTCCACC
CGTATATACTCATTAGCATATGAATAG

Protein sequence predicted based on nucleotide sequence

MVECITPEAIFIGANKQTQVSDIHKVKKIVAFGAGKTIALWDPIEPNNKGVYATLKGHEA
EVTCVRFVPDSDFMVSASEDHHVKIWKFTDYSHLQCIQTIQHYSKTIVALSALPSLISVG
CADGTISIWRQNIQNDEFGLAHEFTIKKGFFYPLCLSLSKVEEKKYLLAIGGTNVNVFIA
SFILSDSGIEKCRVVAELEGHEDWVKSLAFRHQETPGDYLLCSGSQDRYIRLWRIRINDL
IDDSEEDSKKLTLLSNKQYKFQIDDELRVGINFEALIMGHDDWISSLQWHESRLQLLAAT
ADTSLMVWEPDETSGIWVCSLRLGEMSSKGASTATGSSGGFWSCLWFTHERMDFFLTNGK
TGSWRMWATKDNIICDQRLGISGATKDVTDIAWSPSGEYLLATSLDQTTRLFAPWIYDAS
GRKREIATWHEFSRPQIHGYDMICVETVTDTRFVSGGDEKILRSFDLPKGVAGMLQKFVG
IQFEEKSEMPDSATVPVLGLSNKAGEDDANEDDEEEEGGNKETPDITDPLSLLECPPMED
QLQRHLLWPEVEKLYGHGFEITCLDISPDQKLIASACRPNNVQNAVIRIFSTENWLEIKP
ALPFHSLTITRLKFSKDGKFLLSVCRDRKWALWERNMEDNTFELRFKNEKPHTRIIWDAD
WAPLEFGNVFVTASRDKTVKVWRHQKEPADDYVLEASIKHTKAVTAISIHDSMIREKILI
SVGLENGEIYLYSYTLGKFELITQLNEDITPADKITRLRWSHLKRNGKLFLGVGSSDLST
RIYSLAYE*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 2367
Mutations:
None
Discrepancy:
t1735c,S579P
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable