Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
FLH468425.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
ZM552
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
ScCD00101889
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3285nts
Open reading frame: 1 to 3285
ATGAATATGGATTCTGGTATTACAAGTAGTCATGGAAGCATGGATAAGACACAAAAGCAA
AGTTCTGAGTGGGCAGCTAACCAGAAACATAATCAAAGAGTCGAAAATACAAGAGTTTTA
ATGGGCCCCGCTGTCCCTGCGATGCCTCCAGTACCATCAAATTTTCCGCCTGTTCCAACT
GGTACAATAATGTCGCCTCAGTTGAGCCCTTTTCCGGATCACCGTTTAAGACATCACCCA
TTAGCTCATATGATGCCTGCTGATAAGAATTTTCTGGCATATAACATGGAGTCTTTCAAA
AGTAGAGTGACTAAAGCATGTGATTATTGTCGGAAGAGGAAGATTAGATGTACGGAAATC
GAGCCGATTTCTGGTAAATGTAGAAACTGTATCAAGTATAATAAAGATTGCACGTTTCAT
TTCCATGAAGAACTGAAAAGAAGGCGAGAAGAAGCTTTGAATAACAAGGGAAATGGGAAG
TCGGTTAAGAAGCCGAGGTTGGATAAGGAGAATAAGTTCAAGGATGAAAATTTTGATATC
GCCGTGCGATCAAGAAATACTTCTTCTACAGACAGCTCGCCGAAATTACACACCAACTTA
TCACAAGAATATATTGGAGTTTCTGCCGGCAAAAGTGCAAGCGATAAAGAAGATACTTGG
CCTGACTTTGTTCCTATTGACAGGACAGTGCTTGAAAAAATTGAACTCAACCATACGAAG
GTTGCTGGGAAAGTTTTTGTTTTGGAAGAGATTTGTAAAAACATGAAGGGAACAATAGAG
AAGCTAGCTGAAAAAAGCAAAATTGACGTCATTGATAAAGAATACATGAAAAGGCCTAAA
AGAAAGCAATATTCAAAGGCCTTGTTAACAAAACAAAAAATGTTTCATTTTCGACAAAAT
GTTTTATCACATTTAACTGACGAAGAATTTCTTTCGCCCATTAATGAAATGTTTACTACT
ACTTTCAAATATTCTATTTTACAGACCAAGCTGGTCCTGGACTTCTCTTTCCGATCCGCA
TCATCACCATCTAGTGATAATATTTTGTATCCTCTGCCCCGTTTAGCAATTGCAAAGCGT
TTGCTCAAAAATATAAAATGTCCATCATTAGCCTCTCTTCTCCACATAGTGGATGTAGAT
CAATGTTTGCAATTTGCCGACGTGCACTTTGATCCTGCTAAAGGAAGATTGACATCATCA
CAGGCATTTTTGCTAAATATCTGCCTCTGCCTTGGGGCCACCGTTACAAATTTTGAGGAG
AAGCAGGAGCTAGTTGATGAAGATAATCATGAAACCTATTATTTTGAGAAGTTCGAATTA
TGGAGACTCAGATCTTTCACTTTTCTCAATTCTGTCTATTACTACCATAAACTGTCTGTA
GCACGCGCAGATATGACGGCATTAAAGGCATTACTGTTACTGGCAAAGTTTGCCCAACAA
AAAATAAGTGCTTCATCAGCGGTCAAAGTACTATCCGTTGCTATCAAAGTGGCTTTGGAT
TTACGGCTCAATTTACATTCCACCTATGAGGATCTAGAACTTGATGAAATCATTAAAAGA
AGACGCTTGTGGTGTTATTGTTTTTCAACAGACAAATTCTTTTCTGTGGTGTTGTCAAGA
CCGCCCTTTCTCAAGGAGGAAAACACTGATGTATTAACAGATGAAAGCTATGTTGAACTA
TTCAGAGATAAAATTCTGCCCAATCTTTCTATAAAATACGATGATTCAAAACTAGAAGGG
GTGAAAGATATTGTATCCGTTGTAAACTTACTCGCGAATCACCTTGAATATGTACCATAC
ATTCAATCATATTTTTTGTCGAGATTATCCCTCATAGAGTCTCAAATATATTACTCATGT
TTTTCCATCAGAACAACTTTAGACGATACACTAGATGAAATCATTGAAAATGTACTGGAA
AATCAAAAGGCGTTAGATAGAATGCGAGACGATTTGCCCACCATATTATCATTAGAAAAT
TACAAGGAAAACATGAGGATTTTATCTTTGGATTCTTCGAAGTTAGATTTTGAAGTGAGT
TGTTGTACTACTATTCTATTACACCTCAGATGGTACCATCAAAAGATAACCCTGAGTTTA
TTTGTCATATCCATCATTGGAGATAACTTAGATCAACGGGAAAGTTCCCGGCATGATATA
GCTGAAATTATTAGAAGGTCAAGATTGGATTTCAAGCGAAATTGCATCGAAGTACTTAAT
ATTTTGAAAGATTTTGAATACTACCCTACTGTTCAGAATGAGTTTCTGTATTTTTCTCTC
ACAACGGTTTTTTCCATGTTTCTGTATTTGTCAGAGATTATGGTTAATGACGAACATGCC
ATGGAGACTGGTTATATAATTGGATTATTGAGGGATACACATACCCGTATGCTTGGATCT
GAAGAACGCTGTCTTTCAGTACACAATCTGAAGTGGCAAACATCTCTTTTTTTTTACACT
TTTTTTCTACGGAGTACGATGGAAAAGTTCAATCTGACAAGTAAATACGCAAAATTCTAT
GCCTTTGATTCAAATTATTATGAGGGTGTTCTCAATAGGTTAGTTAAGCATACGAGAGAA
TCAAAAGATGATATGGTCGAGCTCTTGAAAACTTCGTTCATAAATAAAGAGAAGATGGCT
GCATTTGGTAGTTTCGTTACAGAGGACCAAGAAAAAATGGAAGTGTCCTTCAATATTTTC
AATGAAATAACAATCCAGGATTTGAACTTCTTGCAATTTTCAAGCATTCCGAAATTGTGG
GAAAATAAAACTCTTGAGCCAGGCGAGGAATATCATCATAGTAATGGTACCAATACTGAT
AATAACGAGACTACTGGCGCTGATGATACTGACGATAATAATAATAATAATAATAATAAT
AATAAAAATGGCAATAATAGTAGTAGTACTATTAATAATAATAACAATAACTACAGTAAC
AGTAACAATAATGACAATGACAACAATATTAATGATGATGATGATGATGATGATGATGAT
GATGATGATGATGATGATGATGATGATGATGATGACAATGATGATGATTATAGTAATAAT
GGTGCTGATGATGATGAAGAAGACGATGACTATGACAGATCTTTATTTCCAACAGGTTTA
GCTTCGCTGCTTGATGCGTCATATCCTGAACGTACGGCGAATGATTATAGAGATGAGAAT
GAACAATCGAATAAATTATTCGAAAAGATTGAAGGCCATTTGGAGCACGGTGTTTTTTTT
TACGATCGGGATTTCTTCTTCAAGAATGTGTGTGTAAAAATGTAG
Protein sequence predicted based on nucleotide sequence
MNMDSGITSSHGSMDKTQKQSSEWAANQKHNQRVENTRVLMGPAVPAMPPVPSNFPPVPT GTIMSPQLSPFPDHRLRHHPLAHMMPADKNFLAYNMESFKSRVTKACDYCRKRKIRCTEI EPISGKCRNCIKYNKDCTFHFHEELKRRREEALNNKGNGKSVKKPRLDKENKFKDENFDI AVRSRNTSSTDSSPKLHTNLSQEYIGVSAGKSASDKEDTWPDFVPIDRTVLEKIELNHTK VAGKVFVLEEICKNMKGTIEKLAEKSKIDVIDKEYMKRPKRKQYSKALLTKQKMFHFRQN VLSHLTDEEFLSPINEMFTTTFKYSILQTKLVLDFSFRSASSPSSDNILYPLPRLAIAKR LLKNIKCPSLASLLHIVDVDQCLQFADVHFDPAKGRLTSSQAFLLNICLCLGATVTNFEE KQELVDEDNHETYYFEKFELWRLRSFTFLNSVYYYHKLSVARADMTALKALLLLAKFAQQ KISASSAVKVLSVAIKVALDLRLNLHSTYEDLELDEIIKRRRLWCYCFSTDKFFSVVLSR PPFLKEENTDVLTDESYVELFRDKILPNLSIKYDDSKLEGVKDIVSVVNLLANHLEYVPY IQSYFLSRLSLIESQIYYSCFSIRTTLDDTLDEIIENVLENQKALDRMRDDLPTILSLEN YKENMRILSLDSSKLDFEVSCCTTILLHLRWYHQKITLSLFVISIIGDNLDQRESSRHDI AEIIRRSRLDFKRNCIEVLNILKDFEYYPTVQNEFLYFSLTTVFSMFLYLSEIMVNDEHA METGYIIGLLRDTHTRMLGSEERCLSVHNLKWQTSLFFYTFFLRSTMEKFNLTSKYAKFY AFDSNYYEGVLNRLVKHTRESKDDMVELLKTSFINKEKMAAFGSFVTEDQEKMEVSFNIF NEITIQDLNFLQFSSIPKLWENKTLEPGEEYHHSNGTNTDNNETTGADDTDDNNNNNNNN NKNGNNSSSTINNNNNNYSNSNNNDNDNNINDDDDDDDDDDDDDDDDDDDDDNDDDYSNN GADDDEEDDDYDRSLFPTGLASLLDASYPERTANDYRDENEQSNKLFEKIEGHLEHGVFF YDRDFFFKNVCVKM*
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
3285
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
yeast
Marker:
LEU2
Host Type: bacterial Marker: ampicillin
Host Type: bacterial Marker: ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
ZM552
Synonyms:
ZM552
Description:
Yeast expression vector with N-terminal 3X V5 epitope tag; ampicillin resistance in bacteria; LEU2 resistance in yeast; Gateway cloning.
Comments:
ZM511 Stu1 vector plus Gateway attR frame B cassette EcoRV fragment; same as ZM515. Cloning into the vector removes the ccdB cassette and chloramphenicol resistance.
Size (bp):
8302
Parent Vector:
None
Properties:
Gateway, acceptor (destination), recombinational cloning, with tag/fusion/marker, yeast expression
Author Name:
Zarmik Moqtaderi
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
primer site
M13F
M13 forward sequencing primer
8286
8301
primer site
M13R
M13 reverse sequencing primer
2630
2647
promoter
CMD1
CMD1 promoter
295
484
recombination site
attR1
attR1 recombinational cloning site
650
666
recombination site
attR2
attR2 recombination site
2337
2352
reporter gene
LacZ
LacZ alpha gene
8146
8214
selectable marker
AmpR
ampcillin resistance
3795
4454
selectable marker
CmR
chloramphenicol resistance. removed with the ccdB cassette in clones with insert
883
1539
selectable marker
LEU2
LEU2 selectable marker in yeast
5273
5344
tag
V5
N-terminal triple V5 tag
494
631
trxn termination sequence
CYC1
CYC1 terminator
2377
2582
yeast ARS
ARS
ARS yeast origin of replication
0
0
yeast centromere
CEN
yeast centromere
0
0
