SPO71 (S. cerevisiae )
In ZM552 (V5-tagged yeast expression vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH469057.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
ZM552
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
ScCD00102138
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3738nts
Open reading frame: 1 to 3738

ATGGATTCTATCGTTAATGTTGTTGAAGACGATGTTAAGTACGCGCAGAGAGTGACTAGC
TTCAGCTCACCACAAAATGCAAATGTCAAGGTCTTCACCATTCCCCGGCATTCCTTCACA
GCGTTTAGACTGTCGTATGTTTCACCTACAGAGCTTTCAGCATGTTCACAGGTGACTTTG
CTAGGTGGTATTCCAAAACAATGGTACGCTGATCAAAATAATCAAGTCTGGAAACTTCTA
ACTAAAATTTCACTTCGGAAGGTTAGGAAGCAATCTGACATGCTAAGAAGATATGGCTAT
GGAACAATTTACAAAAAGCGTGTAGGGAAGATCCCAACTGCATTGTACTTAAGAAAGCAC
TTTACATGGTCCTATGAAGATAATACATCAATTCACAACGGTCATCGTCTTAAAGAAGCG
GAAATGGAGATGAAGAGAACTAGGTCAAGTCCTGTGCAGAAAAGTGAATATAAATTAAGT
CTACCCAGAAGGTGTAGGAGTTCTTCCGATCAAAACTTCATGCGACAAGAGCTTTTGAAA
GAAAAAAAATCAGAGCTTTCTAGAAACAATAGTTTGCCTCTCATCGATACGGCGCAAGCC
GTGGATATCCACCCAGTTTTACATGAAGAAGATCAAGAAAATACAAATAAAAGGAATAAA
TCACTCCTTTCGAATTTGAAACGAAAAGATCTAGGGGAATCAAAAAGCATATCCAGAAAA
GATTATTCGCATTTTGATAGAATACCGAGCCCCTCTTCAGCCAGAAGTGTTGGAGAAACA
GACTTTAATTATAATAGAGAACCTTCTGAAGATACTTTGAGATATCCCGACAGTATAATT
GAAGTAACAAATCGGACAAGCCCCGCCCCAAATAGTATTCTAAGTAGATCAGGACAATTT
GTTAACTCGAATGACCTAAGTGATGGCTTTTCAACATCTAATACTATCAATAACGTGGGA
TTAAATGCAAATGAAAAGATTTTTTTTAATGCTCTACAAAGTATGGAAAAGGAAAACTTG
ATGCTGTGGAAAACATCCCAGAAATATGGGACTTATCTCGATGAAAGACGTAAAAGTGCG
GATGCCTTTCAAAAAAGAGAAAGAGGTTCATGCGTGGACATTGGTAAGTTGCATTCGAGT
CATCTTCCGTTTATTAATATCCTACCTCCATGGCCTACCGAACTGACGGAGGAAGAGAGA
ATTATACACGATAGGCTAGCCTCTAAACATTCTCATCATATAAGAAAACATGTTCACAAT
GCTCGAAACAAAACTAGCTGCAAAATCAAAGATAGTGTTGGAACTTTCTTAGGTATGACA
AATTCACTAACAAATAAAGCAACTGTCAAGAAACGCACCGGTCAGATCCTTAAAAAAGAA
AAAATGCTAGTGATGGTAAAAGAAGCTATACAAAATAAAGTACCACTTCCCAACTTCTCT
GAAAACGAGTGCTTTGATACAAGGGTCAGTGAACGGTGGAAAGAATATATTGTTATTGCA
AGATCCACGGGAAGATTCGATCCCCCGATACTTTTACAGTTCTATCGTCATCGTCATATC
CCAGAAATCGAAGATATCAGTTCCATAGCTACAAAGTATCACCGCAACCCGTTGGATTTT
TTTCTTTCAAGAAATTGTATTGTAAAATTCTACAGCTCTCTAGACAAAACTATATCCATA
CAAAAGCCTGATAAGCGGTTAGGTGGGTTTATAGACGAGAGTATTGAAAAGAAGGACGAA
TTAAAACACTATTCCCCCATTAAAATTTTTATCTTAAGATGCAGCAGTATTCGTTCGTCT
GGTAGATGGTACAAATTTCTACTAGAATCTTTGGACCGACAACTATTTACACCTGCCATC
AACTTGAAAATTCCATTAACTGAGATCTCGATAAAAATAAACTTGAATGAAATTATATTC
CAAAAGCTAATAGATTTAGGGAAGCAGGAAAAAGATAGATTAAAAATATGTTTTCTTCAA
AGAGGTTATAAAATTTTCCAGCATCCCATTTTACGATATTTCACAGTTGCTATTTTGGAG
AAATTAAAATTGGCGCATTATGACTATCTGATTAGAAAATGGGATACAGAAAATCCTGTA
CTAGGATGTGCCCTGAAAAGATATGATAGATTGGAGTGGATTCCATGTGATGAAGATTCT
CTGGTAACTGGAATTTTTGCATTTTGTCAATCTCACCTAATCCAGTACAGGCCAATTGCG
AATCGTTTAAGGGAAACAAAATCTTTAGAAGGAAAATGTCTGAAAGAACCAACACCTATT
GAAGGTTTTTTAATAAGATTGACTGACAAGTATGGCTCTGCGAGGACAAATTTTGGTAAA
TATAGTATTTCAACAGCTTACTTCTTCACGTGTGAGAATTTGCTTTTTTCGATGAAAGCA
TATAGAGCAAACCCACCATTGCCTATAGATTCGATGATTGACGACACTAGCACAGAAATA
GAAAAAGAAGAAATTTGGAAACAGTGGAAGAAAATTCCAGAGGTTTACGAGCAACAACCT
TATCCCTTAGACACAAATGATCACATCGAGTGGATGAACTGTCAAACGACCCAAAGTGAA
TATGATTCCAGAGATTTCTATGCTTTCCATTGTTTTCATAGAAGGATTGATCAAATACTA
AAAACGGACAATGTGATTGACCTAACTGAGGTGAAAGATATATATCAAGGAACAAGGACT
GATTACGAAGCAGACAAAATAAAATATGGGGTTTACAAGGAAGCTAGTGAAATCTTTTGG
CATAGAAACTACGAGATTGATGATGTTTCACGGTCCGTAATAAATATAGAAACCTCAAAT
GGACTCCTCTTGAAGCTGCTTGCTACATCAGCAACAGTTGCTGAAGAGTGGGTCATAAAG
CTGAAACAAATGATATCCTATTGGAAAAACAAGCAAAGGGAAGATACGGAGAGGCTTTTA
AAAATAAGGCGTTCAAATGCAGGTCTTTTGATGCTAAATGGAGAAGAGGAAACAAAAATT
GGTGAGAATACATTGCGCTGGATCGTAGAACATGGGCGTGCAGATGAGCAAACCTTTAAT
GCAAATGGAATATCATTAAGTAGGCCGTTAATTCAAAAGGGACCTTTATATCAAAAGCCG
CACAAGCATTCTGTTTTCTCGAAGTACTACGTCGTTTTGATATCAGGTTTTATTGTACTG
TTCCACTGCTTTCATAGATCAACCACAGGTTTTGCTAAGGAAGTTTTGGAGTATGCTCAT
TATGTAACCATTCCGATTGACGATTGCTACCTATACTCTGGGACCACAACAGAACTAGAC
CTTCTGCAGAGAGATCGTACTTTTGACGAAATAAACTATGGATCCCACGCTCTCCCACGA
GTTTATGGGGACGGATGGCGATCTGTAGAAGACGAGAGTTCGCGATGCTTTACGCTTTGG
TTTGGAACAAGGCGTGCCCTTTCTAGCAATCGCTTACAGAAGAAAGGGAATGAAAAGCAA
TATACGCAAGATTATGGTAGACAAGATAATAATATTGACCCACCTTCTGCTCCGGAAGCT
GACTTGAATAACTCTAATGTTCCAAGTAACACAGATAAGATCCATTTCACTAAAAAGTTA
GGTGTGAGCGGCAAGTCAATGGTTTTCATGGCAAGATCAAGACAGGAAAGAGATCTTTGG
GTAATGTCTATTTACTATGAACTTGAAAGACTAAGAAGAACTGCGTCCACTTCAAATTCA
CGGAATCAAACTATGTAG

Protein sequence predicted based on nucleotide sequence

MDSIVNVVEDDVKYAQRVTSFSSPQNANVKVFTIPRHSFTAFRLSYVSPTELSACSQVTL
LGGIPKQWYADQNNQVWKLLTKISLRKVRKQSDMLRRYGYGTIYKKRVGKIPTALYLRKH
FTWSYEDNTSIHNGHRLKEAEMEMKRTRSSPVQKSEYKLSLPRRCRSSSDQNFMRQELLK
EKKSELSRNNSLPLIDTAQAVDIHPVLHEEDQENTNKRNKSLLSNLKRKDLGESKSISRK
DYSHFDRIPSPSSARSVGETDFNYNREPSEDTLRYPDSIIEVTNRTSPAPNSILSRSGQF
VNSNDLSDGFSTSNTINNVGLNANEKIFFNALQSMEKENLMLWKTSQKYGTYLDERRKSA
DAFQKRERGSCVDIGKLHSSHLPFINILPPWPTELTEEERIIHDRLASKHSHHIRKHVHN
ARNKTSCKIKDSVGTFLGMTNSLTNKATVKKRTGQILKKEKMLVMVKEAIQNKVPLPNFS
ENECFDTRVSERWKEYIVIARSTGRFDPPILLQFYRHRHIPEIEDISSIATKYHRNPLDF
FLSRNCIVKFYSSLDKTISIQKPDKRLGGFIDESIEKKDELKHYSPIKIFILRCSSIRSS
GRWYKFLLESLDRQLFTPAINLKIPLTEISIKINLNEIIFQKLIDLGKQEKDRLKICFLQ
RGYKIFQHPILRYFTVAILEKLKLAHYDYLIRKWDTENPVLGCALKRYDRLEWIPCDEDS
LVTGIFAFCQSHLIQYRPIANRLRETKSLEGKCLKEPTPIEGFLIRLTDKYGSARTNFGK
YSISTAYFFTCENLLFSMKAYRANPPLPIDSMIDDTSTEIEKEEIWKQWKKIPEVYEQQP
YPLDTNDHIEWMNCQTTQSEYDSRDFYAFHCFHRRIDQILKTDNVIDLTEVKDIYQGTRT
DYEADKIKYGVYKEASEIFWHRNYEIDDVSRSVINIETSNGLLLKLLATSATVAEEWVIK
LKQMISYWKNKQREDTERLLKIRRSNAGLLMLNGEEETKIGENTLRWIVEHGRADEQTFN
ANGISLSRPLIQKGPLYQKPHKHSVFSKYYVVLISGFIVLFHCFHRSTTGFAKEVLEYAH
YVTIPIDDCYLYSGTTTELDLLQRDRTFDEINYGSHALPRVYGDGWRSVEDESSRCFTLW
FGTRRALSSNRLQKKGNEKQYTQDYGRQDNNIDPPSAPEADLNNSNVPSNTDKIHFTKKL
GVSGKSMVFMARSRQERDLWVMSIYYELERLRRTASTSNSRNQTM*

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 3738
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  yeast    Marker:  LEU2
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable