Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
FLH464758.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
ZM552
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
ScCD00102842
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6372nts
Open reading frame: 1 to 6372
ATGAGATCTATAAGAAAATGGGCGTACGAGACGTTCAATGATGAAAAAATCATTCAATTC
GTGGTAATGGCGACACCTGATGATTTACACGCAAATTCGGAGTATATTAGAATGGCAGAC
CAATATGTGCAGGTACCAGGGGGTACCAACAACAACAATTACGCCAACATAGACTTAATA
CTGGACGTGGCAGAGCAAACGGATGTGGATGCGGTCTGGGCTGGATGGGGCCATGCTTCT
GAAAATCCGTGTCTTCCTGAGCTGTTAGCTAGTTCACAAAGGAAAATACTATTCATTGGT
CCTCCTGGACGCGCTATGAGATCATTGGGTGACAAGATTTCTTCCACTATTGTAGCACAA
AGCGCTAAAATCCCGTGTATCCCTTGGTCTGGTTCACATATAGACACTATCCATATCGAT
AACAAGACGAACTTTGTATCTGTGCCGGATGATGTATATGTAAGGGGATGTTGTTCCTCA
CCTGAAGATGCTTTAGAAAAGGCTAAATTAATAGGATTTCCTGTAATGATTAAGGCATCC
GAAGGTGGTGGAGGTAAGGGCATTAGGCGAGTAGATAATGAGGATGATTTTATTGCATTA
TATCGCCAAGCAGTGAATGAGACACCTGGGTCGCCTATGTTTGTTATGAAAGTTGTCACT
GATGCTCGTCACTTAGAGGTACAGTTATTAGCTGACCAATATGGCACTAACATTACATTG
TTTGGGAGAGACTGTTCCATACAAAGGCGGCACCAAAAGATTATAGAAGAGGCACCAGTG
ACAATAACCAAGCCTGAAACGTTTCAAAGGATGGAACGCGCAGCAATTCGTCTAGGTGAA
TTGGTAGGTTATGTTTCTGCGGGCACTGTCGAATACTTATATTCACCAAAAGATGATAAA
TTTTACTTTTTAGAACTGAATCCGAGACTACAAGTAGAGCATCCAACGACAGAAATGATA
TCTGGCGTAAACCTTCCTGCCACTCAACTGCAAATCGCCATGGGCATTCCTATGCACATG
ATAAGTGATATCAGAAAACTTTATGGTTTAGATCCAACGGGAACTTCGTATATTGATTTT
AAAAATTTAAAGAGACCCTCGCCAAAAGGCCATTGTATTTCATGCAGGATCACTTCAGAA
GATCCTAATGAAGGTTTCAAGCCCTCCACTGGGAAAATACATGAGCTCAATTTTCGTTCT
TCTTCCAATGTTTGGGGTTACTTCTCAGTAGGAAATAATGGTGCTATTCACTCATTTTCA
GATTCCCAATTTGGGCACATTTTTGCTGTAGGAAACGATAGGCAAGATGCAAAGCAAAAC
ATGGTTTTAGCTCTAAAAGATTTTTCCATCCGAGGAGAATTCAAAACCCCCATAGAGTAC
CTGATAGAGCTATTAGAAACTCGGGACTTTGAGAGTAATAACATATCGACTGGTTGGTTA
GATGATTTGATTTTGAAAAATTTATCTTCCGATAGCAAACTAGATCCAACGCTCGCTATT
ATCTGTGGTGCCGCAATGAAAGCATACGTTTTCACAGAAAAGGTGAGGAATAAGTATTTG
GAATTATTGCGGAGGGGCCAAGTTCCACCTAAAGATTTTCTTAAAACGAAGTTTCCTGTT
GACTTCATTTTCGATAATAATAGATACTTGTTCAATGTTGCTCAATCATCTGAAGAACAA
TTTATTCTTTCTATCAATAAGTCTCAATGTGAAGTTAATGTTCAAAAATTGTCCAGTGAC
TGCTTGTTGATCTCCGTTGACGGTAAATGCCATACAGTTTATTGGAAGGACGATATCAGA
GGTACAAGACTTTCGATAGACTCCAATACCATATTTTTAGAAGCTGAACTCAATCCCACT
CAAGTGATCTCTCCAACTCCGGGGAAATTGGTGAAATATTTGGTCCGAAGTGGTGATCAC
GTTTTTGCTGGACAGCAATATGCAGAAATAGAAATAATGAAAATGCAGATGCCACTAGTA
GCGAAAAGTGATGGTGTAATTGAGTTACTAAGACAGCCCGGTTCCATAATTGAGGCTGGT
GATGTCATCGCAAAATTGACTTTGGATTCGCCGTCCAAAGCTAACGAATCGTCTTTATAC
CGCGGAGAATTACCTGTTTTAGGTCCACCGCTAATAGAGGGTAGCCGACCAAACCATAAG
CTCAGAGTCTTAATAAATAGGTTAGAAAATATTCTCAATGGATATCATGAAAACTCTGGA
ATAGAAACTACTCTAAAAGAGTTGATAAAAATATTGAGAGATGGTAGGCTTCCTTATTCA
GAATGGGATTCCCAAATTTCTACGGTACGCAATAGACTACCAAGGCAATTGAATGAGGGG
CTGGGAAATCTAGTCAAGAAATCTGTTTCTTTTCCTGCAAAGGAACTGCACAAATTAATG
AAGCGCTACTTGGAAGAAAATACAAATGATCATGTAGTTTATGTTGCCTTACAGCCACTT
CTTAAAATTAGTGAAAGGTATAGCGAAGGTTTAGCTAATCACGAATGTGAAATTTTTTTA
AAGTTGATTAAAAAGTACTATGCTGTTGAGAAAATTTTTGAAAATCATGATATACATGAA
GAAAGAAACTTACTAAATCTGCGGAGGAAAGACCTTACAAACTTAAAAGAAATTTTGTGC
ATAAGTTTATCGCATGCTAACGTAGTCGCAAAGAACAAGTTAGTAACTGCAATATTGCAT
GAATACGAGCCATTGTGCCAGGATTCCTCTAAGATGTCTTTAAAATTCAGGGCTGTTATA
CATGATTTGGCAAGTTTGGAATCTAAGTGGGCTAAAGAGGTTGCTGTAAAGGCAAGATCA
GTGCTACTCAGAGGGATTTTCCCTCCCATAAAGAAAAGAAAAGAGCATATTAAAACTCTC
CTGCAATTGCACATAAAGGATACTGGTGCCGAAAACATTCACAGCAGGAACATATATTCC
TGTATGAGGGATTTTGGTAATTTAATACATTCAAATCTGATACAACTTCAGGATTTGTTC
TTTTTTTTTGGCCATCAAGATACGGCTCTTTCCAGTATAGCATCTGAAATTTATGCAAGG
TATGCCTACGGCAATTATCAATTAAAAAGTATTAAGATTCATAAAGGAGCGCCTGATTTA
CTAATGTCATGGCAATTCAGCTCATTAAGAAATTATTTAGTCAATTCTGATGGTGAGAGT
GATGAGTTTACAAAACTTTCTAAACCTCCCTCAACATCAGGTAAGAGCTCAGCAAATAGT
TTTGGTCTTCTTGTCAACATGCGTGCGCTTGAATCTCTGGAAAAGACATTAGACGAGGTA
TACGAACAAATTCATATTCCTGAGGAAAGACTTTCCAGCGGAGAGAACTCTCTTATTGTT
AATATTTTATCTCCTATTCGTTACAGAAGTGAAAATGATCTAATTAAAACTTTAAAAATT
AAACTTCATGAAAATGAGAGAGGTCTATCCAAGCTCAAGGTTAATCGTATTACATTTGCA
TTTATCGCCGCGAATGCGCCCGCTGTTAAATTTTACTCCTTTGATGGAACTACGTACGAT
GAAATCTCTCAAATAAGAAATATGGATCCATCCTATGAAGCACCGTTAGAGTTAGGAAAA
ATGTCGAACTATAAAATCAGATCACTACCTACATACGATAGTAGTATACGCATTTTTGAA
GGTATTAGCAAATTTACGCCGCTAGATAAAAGGTTCTTTGTCAGGAAAATCATAAATTCC
TTCATGTATAATGATCAAAAAACAACCGAAGAAAACTTGAAAGCGGAAATCAATGCTCAA
GTGGTTTATATGTTAGAACATCTAGGAGCAGTTGACATCTCAAATTCAGACTTGAATCAT
ATTTTTTTAAGTTTCAATACAGTTCTTAACATACCAGTACATCGTCTCGAGGAAATTGTG
AGTACAATTCTAAAGACTCACGAAACCAGATTGTTTCAAGAAAGAATCACAGATGTAGAA
ATTTGCATCTCTGTTGAGTGCCTAGAAACAAAGAAGCCAGCCCCGCTTAGATTACTTATT
TCTAATAAATCTGGGTATGTGGTAAAAATTGAGACATATTACGAAAAGATAGGGAAAAAT
GGGAATCTGATTTTGGAACCGTGTAGTGAGCAGAGCCATTATAGCCAGAAATCTCTCTCT
CTTCCTTACTCGGTCAAGGATTGGCTACAACCTAAAAGGTACAAAGCTCAATTCATGGGT
ACAACATATGTGTACGATTTCCCAGGTCTGTTTCATCAAGCTGCAATCCAACAGTGGAAA
AGGTATTTTCCAAAACATAAGCTGAATGACAGTTTTTTTAGTTGGGTTGAATTGATAGAA
CAAAACGGTAATTTAATAAAAGTAAACAGGGAGCCAGGCCTTAATAATATAGGGATGGTT
GCTTTTGAGATTATGGTTCAGACACCTGAATATCCTGAAGGGCGTAACATGATCGTGATT
TCTAATGATATTACCTACAATATTGGATCTTTTGGACCGAGAGAAGATTTGTTTTTTGAT
AGGGTCACAAATTATGCAAGAGAGAGAGGGATCCCGAGGATATACTTGGCGGCGAATTCA
GGAGCTAAATTGGGTATAGCCGAAGAGCTGATCCCTCTATTTCGTGTAGCATGGAATGAC
CCCTCTGATCCAACAAAGGGTTTCCAGTACTTATACTTAGCTCCAAAAGACATGCAGCTA
CTGAAAGATTCTGGGAAAGGAAATTCGGTTGTTGTTGAACACAAGATGGTATACGGTGAA
GAGAGATATATTATTAAAGCAATAGTCGGATTCGAAGAGGGTTTAGGTGTTGAATGTTTA
CAGGGCTCGGGTTTAATTGCTGGTGCCACTTCGAAAGCGTATAGAGACATTTTCACTATT
ACTGCTGTTACTTGTCGGTCCGTTGGTATAGGTTCCTATCTGGTCAGACTAGGACAACGT
ACTATTCAGGTGGAGGATAAGCCTATCATACTGACGGGTGCATCGGCGATTAATAAAGTT
TTGGGTACCGATATCTATACATCTAACCTACAAATTGGCGGAACCCAAATCATGTATAAA
AACGGAATAGCGCATTTAACAGCCAGTAATGATATGAAAGCCATCGAAAAAATAATGACA
TGGTTATCATATGTCCCGGCGAAAAGAGATATGAGTCCTCCACTTCTTGAAACTATGGAT
AGATGGGATAGGGATGTAGACTTCAAACCTGCCAAGCAAGTGCCATATGAGGCAAGGTGG
TTGATAGAGGGTAAATGGGACTCAAATAACAACTTCCAGTCAGGCCTATTTGATAAGGAT
TCGTTTTTTGAGACATTATCTGGATGGGCCAAAGGTGTAATAGTTGGAAGAGCACGTCTT
GGAGGTATTCCCGTGGGTGTTATTGCGGTAGAAACTAAGACTATCGAAGAAATAATCCCC
GCTGACCCAGCTAATCTGGATTCTTCAGAGTTTTCCGTTAAAGAAGCAGGACAGGTGTGG
TATCCAAATTCCGCGTTCAAAACAGCTCAAACTATAAATGATTTTAACTATGGTGAGCAA
TTACCATTGATTATCTTAGCCAATTGGAGGGGATTTTCTGGCGGTCAAAGGGATATGTAC
AATGAAGTACTAAAGTACGGGTCTTTTATTGTTGACGCTCTGGTTGACTACAAACAACCC
ATACTGATATACATTCCGCCCTTTGGTGAATTAAGGGGCGGATCATGGGTTGTTATAGAT
CCAACTATTAATCCTGAACAAATGGAAATGTATGCCGATGTTGAATCTAGGGGAGGTGTG
TTAGAACCTGACGGAGTAGTTAGCATAAAATACCGTAAGGAGAAAATGATAGAGACGATG
ATTCGATTAGACTCCACATATGGACATTTGAGAAGAACGTTGACAGAAAAAAAGTTATCT
TTGGAAAAACAAAATGATCTTACGAAGAGATTGAAAATAAGAGAGAGACAGTTGATACCA
ATTTACAATCAAATCAGCATACAGTTTGCAGATTTACATGATAGATCGACTAGGATGCTA
GTTAAAGGAGTAATCCGAAATGAGTTGGAATGGAAAAAGTCACGCAGATTTTTATATTGG
AGACTGAGAAGGAGGTTGAACGAGGGACAAGTGATCAAAAGACTGCAAAAAAAAACATGT
GATAACAAAACGAAAATGAAGTACGACGACCTGTTGAAAATAGTTCAGTCATGGTATAAC
GATCTGGATGTTAATGATGACAGAGCAGTAGTGGAGTTCATAGAAAGAAATTCGAAAAAA
ATTGACAAGAACATTGAAGAGTTTGAGATCTCGCTGTTGATCGATGAGCTTAAGAAAAAA
TTTGAAGACAGAAGGGGAAACATTGTCCTTGAAGAGCTAACTAGGTTGGTGGACAGTAAG
CGAAAGAGATAG
Protein sequence predicted based on nucleotide sequence
MRSIRKWAYETFNDEKIIQFVVMATPDDLHANSEYIRMADQYVQVPGGTNNNNYANIDLI LDVAEQTDVDAVWAGWGHASENPCLPELLASSQRKILFIGPPGRAMRSLGDKISSTIVAQ SAKIPCIPWSGSHIDTIHIDNKTNFVSVPDDVYVRGCCSSPEDALEKAKLIGFPVMIKAS EGGGGKGIRRVDNEDDFIALYRQAVNETPGSPMFVMKVVTDARHLEVQLLADQYGTNITL FGRDCSIQRRHQKIIEEAPVTITKPETFQRMERAAIRLGELVGYVSAGTVEYLYSPKDDK FYFLELNPRLQVEHPTTEMISGVNLPATQLQIAMGIPMHMISDIRKLYGLDPTGTSYIDF KNLKRPSPKGHCISCRITSEDPNEGFKPSTGKIHELNFRSSSNVWGYFSVGNNGAIHSFS DSQFGHIFAVGNDRQDAKQNMVLALKDFSIRGEFKTPIEYLIELLETRDFESNNISTGWL DDLILKNLSSDSKLDPTLAIICGAAMKAYVFTEKVRNKYLELLRRGQVPPKDFLKTKFPV DFIFDNNRYLFNVAQSSEEQFILSINKSQCEVNVQKLSSDCLLISVDGKCHTVYWKDDIR GTRLSIDSNTIFLEAELNPTQVISPTPGKLVKYLVRSGDHVFAGQQYAEIEIMKMQMPLV AKSDGVIELLRQPGSIIEAGDVIAKLTLDSPSKANESSLYRGELPVLGPPLIEGSRPNHK LRVLINRLENILNGYHENSGIETTLKELIKILRDGRLPYSEWDSQISTVRNRLPRQLNEG LGNLVKKSVSFPAKELHKLMKRYLEENTNDHVVYVALQPLLKISERYSEGLANHECEIFL KLIKKYYAVEKIFENHDIHEERNLLNLRRKDLTNLKEILCISLSHANVVAKNKLVTAILH EYEPLCQDSSKMSLKFRAVIHDLASLESKWAKEVAVKARSVLLRGIFPPIKKRKEHIKTL LQLHIKDTGAENIHSRNIYSCMRDFGNLIHSNLIQLQDLFFFFGHQDTALSSIASEIYAR YAYGNYQLKSIKIHKGAPDLLMSWQFSSLRNYLVNSDGESDEFTKLSKPPSTSGKSSANS FGLLVNMRALESLEKTLDEVYEQIHIPEERLSSGENSLIVNILSPIRYRSENDLIKTLKI KLHENERGLSKLKVNRITFAFIAANAPAVKFYSFDGTTYDEISQIRNMDPSYEAPLELGK MSNYKIRSLPTYDSSIRIFEGISKFTPLDKRFFVRKIINSFMYNDQKTTEENLKAEINAQ VVYMLEHLGAVDISNSDLNHIFLSFNTVLNIPVHRLEEIVSTILKTHETRLFQERITDVE ICISVECLETKKPAPLRLLISNKSGYVVKIETYYEKIGKNGNLILEPCSEQSHYSQKSLS LPYSVKDWLQPKRYKAQFMGTTYVYDFPGLFHQAAIQQWKRYFPKHKLNDSFFSWVELIE QNGNLIKVNREPGLNNIGMVAFEIMVQTPEYPEGRNMIVISNDITYNIGSFGPREDLFFD RVTNYARERGIPRIYLAANSGAKLGIAEELIPLFRVAWNDPSDPTKGFQYLYLAPKDMQL LKDSGKGNSVVVEHKMVYGEERYIIKAIVGFEEGLGVECLQGSGLIAGATSKAYRDIFTI TAVTCRSVGIGSYLVRLGQRTIQVEDKPIILTGASAINKVLGTDIYTSNLQIGGTQIMYK NGIAHLTASNDMKAIEKIMTWLSYVPAKRDMSPPLLETMDRWDRDVDFKPAKQVPYEARW LIEGKWDSNNNFQSGLFDKDSFFETLSGWAKGVIVGRARLGGIPVGVIAVETKTIEEIIP ADPANLDSSEFSVKEAGQVWYPNSAFKTAQTINDFNYGEQLPLIILANWRGFSGGQRDMY NEVLKYGSFIVDALVDYKQPILIYIPPFGELRGGSWVVIDPTINPEQMEMYADVESRGGV LEPDGVVSIKYRKEKMIETMIRLDSTYGHLRRTLTEKKLSLEKQNDLTKRLKIRERQLIP IYNQISIQFADLHDRSTRMLVKGVIRNELEWKKSRRFLYWRLRRRLNEGQVIKRLQKKTC DNKTKMKYDDLLKIVQSWYNDLDVNDDRAVVEFIERNSKKIDKNIEEFEISLLIDELKKK FEDRRGNIVLEELTRLVDSKRKR*
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
6372
Mutations:
None
Discrepancy:
g2625a; a2629g,K877E
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
yeast
Marker:
LEU2
Host Type: bacterial Marker: ampicillin
Host Type: bacterial Marker: ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
ZM552
Synonyms:
ZM552
Description:
Yeast expression vector with N-terminal 3X V5 epitope tag; ampicillin resistance in bacteria; LEU2 resistance in yeast; Gateway cloning.
Comments:
ZM511 Stu1 vector plus Gateway attR frame B cassette EcoRV fragment; same as ZM515. Cloning into the vector removes the ccdB cassette and chloramphenicol resistance.
Size (bp):
8302
Parent Vector:
None
Properties:
Gateway, acceptor (destination), recombinational cloning, with tag/fusion/marker, yeast expression
Author Name:
Zarmik Moqtaderi
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
primer site
M13F
M13 forward sequencing primer
8286
8301
primer site
M13R
M13 reverse sequencing primer
2630
2647
promoter
CMD1
CMD1 promoter
295
484
recombination site
attR1
attR1 recombinational cloning site
650
666
recombination site
attR2
attR2 recombination site
2337
2352
reporter gene
LacZ
LacZ alpha gene
8146
8214
selectable marker
AmpR
ampcillin resistance
3795
4454
selectable marker
CmR
chloramphenicol resistance. removed with the ccdB cassette in clones with insert
883
1539
selectable marker
LEU2
LEU2 selectable marker in yeast
5273
5344
tag
V5
N-terminal triple V5 tag
494
631
trxn termination sequence
CYC1
CYC1 terminator
2377
2582
yeast ARS
ARS
ARS yeast origin of replication
0
0
yeast centromere
CEN
yeast centromere
0
0
