SIR3 (S. cerevisiae ) in pBY011 (yeast expression vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH113396.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pBY011
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
ScCD00010360
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2937nts
Open reading frame: 1 to 2937

ATGGCTAAAACATTGAAAGATTTGGACGGTTGGCAAGTTATCATTACAGATGATCAGGGG
AGGGTTATTGACGACAACAATAGAAGAAGATCAAGAAAAAGAGGAGGAGAAAACGTATTT
CTGAAAAGAATTTCTGATGGATTGAGTTTTGGGAAGGGTGAGAGCGTGATATTTAATGAT
AATGTGACGGAAACATATTCTGTCTACTTGATCCATGAGACCAGACTCAATACACTTAAT
AATGTTGTGGAGATTTGGGTTTTTTCTTACTTGAGATGGTTTGAACTCAAACCAAAACTG
TACTACGAACAATTCAGGCCAGATCTAATAAAGGAAGATCATCCTTTAGAATTTTACAAA
GATAAATTTTTCAACGAAGTAAATAAGAGCGAGTTATATTTAACTGCTGAGCTATCAGAG
ATTTGGCTTAAGGATTTCATTGCAGTTGGACAGATACTGCCAGAGTCACAATGGAATGAT
AGTAGTATCGATAAAATAGAGGATAGAGATTTTCTAGTACGTTATGCATGCGAACCTACT
GCGGAAAAGTTTGTACCAATTGATATTTTTCAAATCATAAGAAGAGTAAAGGAAATGGAA
CCCAAACAATCTGATGAATACTTGAAAAGAGTATCTGTACCAGTGAGTGGGCAGAAGACA
AATAGACAGGTGATGCATAAGATGGGAGTTGAAAGATCTTCAAAAAGACTAGCAAAGAAA
CCTTCAATGAAAAAAATTAAAATCGAGCCCTCTGCAGATGATGACGTAAATAATGGAAAT
ATACCGTCTCAGCGAGGAACGTCAACAACTCATGGTTCAATTTCTCCCCAAGAAGAGTCT
GTTTCCCCTAACATCTCATCGGCGTCCCCTTCTGCGCTCACATCACCTACAGATTCTTCG
AAAATTTTACAAAAAAGATCTATATCAAAGGAACTAATTGTTTCAGAGGAAATACCAATA
AACTCTTCAGAACAGGAATCCGATTATGAGCCAAACAATGAAACATCTGTGCTTTCAAGT
AAACCTGGGTCAAAGCCAGAGAAGACATCTACAGAACTCGTTGACGGCCGAGAGAATTTT
GTATATGCAAATAATCCAGAAGTGAGTGATGATGGTGGGCTTGAAGAGGAAACAGATGAA
GTTAGTTCAGAAAGTTCTGATGAAGCAATAATACCAGTAAATAAAAGACGTGGCGCTCAC
GGAAGCGAATTGAGCAGTAAAATTAGAAAAATCCACATTCAAGAAACACAAGAATTTTCT
AAAAATTACACTACAGAAACAGATAACGAAATGAACGGAAATGGAAAACCTGGAATTCCC
AGGGGCAACACCAAAATTCATAGTATGAACGAAAATCCTACACCAGAAAAAGGTAATGCA
AAGATGATTGATTTCGCTACTTTGTCAAAGTTGAAAAAAAAATATCAGATAATTTTAGAC
CGGTTTGCACCAGATAATCAAGTAACAGATTCCTCCCAATTGAACAAATTAACAGACGAA
CAGTCAAGCTTGGACGTTGCAGGCCTTGAGGATAAGTTTAGAAAAGCATGCTCTTCATCT
GGAAGAGAAACCATTCTGTCAAATTTTAACGCTGATATAAATTTAGAGGAATCAATTAGA
GAATCTTTACAAAAGAGAGAACTACTAAAATCACAAGTGGAGGATTTTACAAGAATATTC
CTTCCGATTTACGACAGTTTGATGTCTTCCCAAAATAAACTATTTTATATTACCAATGCA
GATGATTCTACTAAATTCCAGCTTGTGAATGATGTAATGGATGAGTTGATCACCTCTTCG
GCCCGAAAGGAACTACCTATTTTCGATTACATTCATATTGATGCCTTGGAACTGGCAGGA
ATGGATGCATTATATGAGAAAATATGGTTCGCCATTTCTAAAGAAAATCTATGCGGTGAT
ATATCACTAGAGGCCTTAAACTTCTATATTACAAATGTCCCGAAAGCAAAAAAGAGAAAG
ACACTAATATTAATACAAAATCCGGAAAATCTATTGAGTGAGAAGATTTTACAATATTTC
GAGAAATGGATTTCTTCGAAAAATTCAAAATTGTCTATCATTTGCGTTGGCGGACATAAT
GTGACGATTAGAGAGCAAATAAATATAATGCCCTCCCTCAAAGCACACTTTACCGAAATC
AAACTTAATAAGGTGGACAAGAATGAATTGCAACAAATGATCATCACGCGACTCAAATCT
TTATTGAAACCTTTTCATGTCAAAGTAAATGATAAGAAGGAAATGACTATTTACAATAAT
ATTCGCGAAGGACAGAACCAGAAAATACCAGATAATGTGATAGTAATCAATCATAAGATC
AACAACAAAATCACTCAACTTATTGCAAAGAACGTAGCCAACGTAAGTGGTAGTACTGAA
AAAGCATTCAAAATATGTGAAGCTGCCGTAGAAATTTCTAAAAAAGACTTCGTACGGAAA
GGTGGTCTACAAAAGGGTAAATTGGTAGTGTCACAGGAGATGGTACCACGCTATTTCTCA
GAAGCTATTAACGGATTCAAGGATGAAACTATTTCAAAGAAGATTATAGGAATGTCTCTG
TTGATGAGAACATTTTTATATACCCTGGCGCAAGAAACCGAAGGCACGAATCGTCATACG
CTTGCTCTGGAGACCGTCCTGATTAAGATGGTGAAGATGTTGCGGGACAACCCAGGGTAC
AAGGCGTCAAAAGAAATTAAGAAGGTCATATGCGGTGCCTGGGAGCCCGCAATAACTATA
GAGAAACTAAAGCAATTTTCTTGGATAAGTGTAGTGAATGATTTAGTGGGAGAAAAATTA
GTTGTTGTGGTGCTCGAGGAGCCCAGTGCAAGCATTATGGTAGAACTAAAACTTCCTTTA
GAAATAAATTACGCCTTTTCGATGGATGAAGAATTCAAAAATATGGACTGCATTTGA

Protein sequence predicted based on nucleotide sequence

MAKTLKDLDGWQVIITDDQGRVIDDNNRRRSRKRGGENVFLKRISDGLSFGKGESVIFND
NVTETYSVYLIHETRLNTLNNVVEIWVFSYLRWFELKPKLYYEQFRPDLIKEDHPLEFYK
DKFFNEVNKSELYLTAELSEIWLKDFIAVGQILPESQWNDSSIDKIEDRDFLVRYACEPT
AEKFVPIDIFQIIRRVKEMEPKQSDEYLKRVSVPVSGQKTNRQVMHKMGVERSSKRLAKK
PSMKKIKIEPSADDDVNNGNIPSQRGTSTTHGSISPQEESVSPNISSASPSALTSPTDSS
KILQKRSISKELIVSEEIPINSSEQESDYEPNNETSVLSSKPGSKPEKTSTELVDGRENF
VYANNPEVSDDGGLEEETDEVSSESSDEAIIPVNKRRGAHGSELSSKIRKIHIQETQEFS
KNYTTETDNEMNGNGKPGIPRGNTKIHSMNENPTPEKGNAKMIDFATLSKLKKKYQIILD
RFAPDNQVTDSSQLNKLTDEQSSLDVAGLEDKFRKACSSSGRETILSNFNADINLEESIR
ESLQKRELLKSQVEDFTRIFLPIYDSLMSSQNKLFYITNADDSTKFQLVNDVMDELITSS
ARKELPIFDYIHIDALELAGMDALYEKIWFAISKENLCGDISLEALNFYITNVPKAKKRK
TLILIQNPENLLSEKILQYFEKWISSKNSKLSIICVGGHNVTIREQINIMPSLKAHFTEI
KLNKVDKNELQQMIITRLKSLLKPFHVKVNDKKEMTIYNNIREGQNQKIPDNVIVINHKI
NNKITQLIAKNVANVSGSTEKAFKICEAAVEISKKDFVRKGGLQKGKLVVSQEMVPRYFS
EAINGFKDETISKKIIGMSLLMRTFLYTLAQETEGTNRHTLALETVLIKMVKMLRDNPGY
KASKEIKKVICGAWEPAITIEKLKQFSWISVVNDLVGEKLVVVVLEEPSASIMVELKLPL
EINYAFSMDEEFKNMDCI*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 2937
Mutations:
None
Discrepancy:
t221c,I74T
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  yeast    Marker:  URA
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable