PRP5 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH113543.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
ScCD00010421
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2550nts
Open reading frame: 1 to 2550

ATGGAAACTATTGATTCGAAGCAAAATATTAATAGGGAGTCTTTATTGGAGGAAAGGAGG
AAAAAACTAGCAAAATGGAAACAAAAAAAAGCACAATTTGATGCTCAGAAAGAGCATCAA
ACTTCACGCAATGACATTGTTACTAACAGTTTAGAAGGTAAGCAGACTACTGAAAAGTTT
ACGGAAAGACAGGAACGTGTAAAAGAAGAGCTTCGGAAGAGAAAAAATGAATTTAGGAAA
TCTGACGAACCTGTATCGGTTAAGCCTTCGAAGAAAAAGTCGAAGAGAAGTAAGGTAAAG
AAGAAAATATCCTTCGACTTCAGTGATGACGATGATTCTGAAATAGGAGTTTCTTTTAGA
TCGAAGGAGCACATTCAAAAAGCTCCAGAGCATGATAATGAAAAAGATCCACTGGATGAG
TTTATGACGTCATTGAAGGAGGAGAAAATGAGTAATAGTAAAGGCATGTACGACAGAGGC
GACATTCTTGATGTAGAGGATCAATTGTTTGAACTTGGAGGAACCGACGATGAGGATGTT
GAAGATAATACGGATAATTCTAATATAGCGAAAATTGCTAAACTCAAAGCAAAAAAACGT
GTAAAGCAAATTTATTACTCTCCAGAAGAGCTTGAGCCGTTCCAAAAAAATTTTTATATA
GAATCTGAAACAGTTTCCTCGATGTCAGAGATGGAAGTTGAGGAGCTTAGACTTAGTCTG
GATAATATCAAAATAAAGGGAACAGGTTGCCCCAAACCAGTCACAAAGTGGTCTCAACTG
GGACTATCAACGGACACCATGGTTTTAATTACAGAAAAGTTGCACTTTGGCTCCTTAACG
CCTATTCAATCCCAGGCTCTTCCCGCTATTATGTCAGGTCGTGATGTTATAGGAATATCA
AAAACTGGCTCCGGCAAGACCATCTCCTATCTTTTACCATTGCTAAGACAAGTAAAAGCT
CAACGGCCATTATCAAAACATGAAACAGGGCCCATGGGTTTAATTCTGGCTCCAACTAGA
GAGCTAGCTTTACAAATACATGAAGAAGTTACTAAATTCACAGAAGCAGATACATCCATT
AGATCAGTATGCTGTACAGGAGGTTCTGAAATGAAAAAGCAGATTACTGATCTTAAAAGA
GGCACTGAGATTGTTGTTGCCACACCGGGACGATTTATTGATATATTAACACTAAATGAT
GGGAAATTACTTAGTACTAAAAGAATAACGTTCGTAGTAATGGATGAGGCAGACAGGCTG
TTCGATTTAGGTTTTGAACCTCAAATAACGCAAATCATGAAAACTGTTCGACCGGATAAA
CAGTGTGTTCTATTTAGTGCAACTTTTCCGAACAAACTACGCAGTTTTGCCGTAAGAGTT
TTGCATTCCCCAATATCTATTACGATTAATTCAAAGGGAATGGTTAATGAAAACGTAAAA
CAAAAGTTTAGAATATGCCATTCAGAAGATGAAAAATTTGACAATCTGGTGCAGCTTATC
CATGAGCGCAGTGAATTTTTTGACGAGGTTCAAAGTGAAAATGACGGACAGTCAAGCGAT
GTTGAAGAAGTTGATGCTAAAGCCATTATATTTGTATCAAGTCAGAATATTTGCGACTTC
ATTTCAAAAAAGCTGTTAAATGCTGGAATTGTGACCTGTGCTATTCATGCAGGTAAGCCA
TATCAAGAAAGGCTTATGAACTTAGAAAAGTTCAAACGAGAAAAGAACAGTATTCTTCTC
TGTACAGAGGTTCTCTCAAGAGGTTTAAATGTTCCCGAAGTGTCGTTGGTTATTATCTAT
AACGCCGTCAAAACTTTCGCGCAATATGTTCATACTACTGGAAGAACTGCCAGAGGTAGT
CGCTCCGGTACTGCTATTACTCTTCTATTGCATGATGAACTGTCAGGTGCTTATATCTTG
AGCAAGGCAATGCGCGATGAAGAAATAAAAGCTTTAGACCCTCTGCAAGCAAAAGAACTT
CAGGAAATGAGTGCCAAGTTTGAATCCGGTATGAAAAAGGGTAAGTTTAGGTTGTCGAAA
GGGTTCGGCGGCAAAGGACTGGAAAATATCAAAAGTAAAAGAGAGGAAGCTCAAAATAAA
GATTTGGAGCTCAAAAAGAATGATAAGAGGAGCGATGACTTGGAAAAGAAAATCAGCAAT
CCACGCGAAGGGCATGACAGTGTGTCTGAGTCTTCTGCGCTTATTCCGAGACTCAATTAT
GAACTATTCAAAGAGTCTACAGACGGGTCGATCATCTTTTACGCCAAGGTTTATATTAAT
GATTTACCCCAGATTGTAAGATGGGAGGCTACAAAGAATACAACCTTATTATTTATCAAG
CATGAAACCGGATGTAGTATTACTAACAAGGGTAAATTTTACCCTGAAGGGAAGGAACCA
AAGAATGAAAACGATGAGCCTAAACTATACTTATTGATCGAAGGCCAAGATGAGAAAGAC
ATACAATTAAGTATAGAATTGCTGGAGCAAAAAGTTAAAGAGGGGGTCGTAAAGGCTGCA
AGCTTGTCTTTGAAGAGTACTAAATACTAG

Protein sequence predicted based on nucleotide sequence

METIDSKQNINRESLLEERRKKLAKWKQKKAQFDAQKEHQTSRNDIVTNSLEGKQTTEKF
TERQERVKEELRKRKNEFRKSDEPVSVKPSKKKSKRSKVKKKISFDFSDDDDSEIGVSFR
SKEHIQKAPEHDNEKDPLDEFMTSLKEEKMSNSKGMYDRGDILDVEDQLFELGGTDDEDV
EDNTDNSNIAKIAKLKAKKRVKQIYYSPEELEPFQKNFYIESETVSSMSEMEVEELRLSL
DNIKIKGTGCPKPVTKWSQLGLSTDTMVLITEKLHFGSLTPIQSQALPAIMSGRDVIGIS
KTGSGKTISYLLPLLRQVKAQRPLSKHETGPMGLILAPTRELALQIHEEVTKFTEADTSI
RSVCCTGGSEMKKQITDLKRGTEIVVATPGRFIDILTLNDGKLLSTKRITFVVMDEADRL
FDLGFEPQITQIMKTVRPDKQCVLFSATFPNKLRSFAVRVLHSPISITINSKGMVNENVK
QKFRICHSEDEKFDNLVQLIHERSEFFDEVQSENDGQSSDVEEVDAKAIIFVSSQNICDF
ISKKLLNAGIVTCAIHAGKPYQERLMNLEKFKREKNSILLCTEVLSRGLNVPEVSLVIIY
NAVKTFAQYVHTTGRTARGSRSGTAITLLLHDELSGAYILSKAMRDEEIKALDPLQAKEL
QEMSAKFESGMKKGKFRLSKGFGGKGLENIKSKREEAQNKDLELKKNDKRSDDLEKKISN
PREGHDSVSESSALIPRLNYELFKESTDGSIIFYAKVYINDLPQIVRWEATKNTTLLFIK
HETGCSITNKGKFYPEGKEPKNENDEPKLYLLIEGQDEKDIQLSIELLEQKVKEGVVKAA
SLSLKSTKY*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 2550
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable