HOS4 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH202741.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
ScCD00013112
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3252nts
Open reading frame: 1 to 3252

ATGAATGAGACTACTACAAAACAGCCGCTAAAGAAAAGGTCCCTGAGCAGCTATCTTTCA
AACGTGAGCACGAGACGGGAGGAGCTGGAAAAGATTTCTAAACAAGAAACATCCGAAGAG
GAAGATACTGCCGGCAAGCATGAACAAAGGGAAACACTGTCGGAAGAAGTAAGTGATAAG
TTTCCAGAAAATGTTGCATCGTTTCGCTCACAGACTACAAGCGTTCATCAAGCCACCCAA
AATAACCTGAATGCAAAGGAATCCGAAGACCTGGCCCATAAGAATGATGCGAGTTCACAC
GAAGGAGAGGTTAATGGGGACAGCCGTCCGGACGATGTTCCTGAAACGAATGAAAAAATA
AGCCAAGCAATACGAGCGAAAATTTCGTCATCATCATCATCACCCAATGTACGTAATGTT
GATATCCAAAACCATCAACCTTTTTCGAGAGACCAACTTCGAGCGATGTTGAAAGAACCA
AAAAGGAAAACTGTTGATGATTTCATAGAAGAAGAGGGTTTGGGAGCTGTTGAAGAAGAG
GATTTAAGTGATGAGGTACTTGAAAAAAATACAACAGAACCAGAAAATGTGGAAAAAGAT
ATAGAGTATAGCGACTCAGATAAGGACACAGACGATGTGGGAAGCGATGATCCCACGGCA
CCCAACTCACCAATAAAACTTGGTCGTCGCAAACTGGTTAGAGGTGACCAACTTGATGCA
ACAACAAGTTCCATGTTTAATAACGAATCAGATTCTGAATTATCAGATATCGATGATAGC
AAGAATATTGCTTTATCCAGTAGCCTATTTAGAGGCGGTTCTTCACCTGTGAAAGAGACA
AACAACAACCTTTCAAATATGAATTCTTCACCAGCACAAAATCCAAAAAGGGGTTCTGTC
TCCAGGAGTAATGATAGTAACAAGAGTTCTCATATAGCTGTTTCCAAGCGCCCCAAACAG
AAGAAGGGCATATATAGGGATTCTGGTGGTAGAACAAGACTACAGATTGCCTGTGACAAG
GGCAAATATGATGTAGTCAAAAAGATGATTGAAGAAGGAGGTTACGATATTAATGACCAA
GACAATGCTGGTAATACAGCATTGCATGAAGCGGCGTTGCAAGGTCATATTGAGATTGTG
GAACTGTTGATAGAAAATGGTGCAGATGTAAATATCAAGTCTATTGAAATGTTCGGCGAT
ACTCCCTTGATCGATGCTTCCGCCAATGGACACTTGGATGTTGTCAAGTATCTTCTTAAA
AACGGTGCGGACCCAACTATACGTAACGCTAAAGGGTTAACTGCGTTTGAATCTGTCGAT
GATGAATCTGAATTTGATGATGAAGAAGACCAAAAGATTTTGCGTGAAATAAAAAAAAGG
TTGAGTATAGCCGCTAAAAAATGGACTAACAGAGCAGGAATTCATAATGACAAATCTAAA
AATGGCAATAATGCTCACACAATAGATCAGCCACCTTTTGATAATACCACAAAAGCCAAA
AACGAAAAGGCCGCTGACTCACCTTCAATGGCTTCCAATATTGATGAGAAAGCTCCGGAA
GAGGAATTCTATTGGACAGATGTTACTTCTAGAGCGGGAAAAGAAAAACTGTTTAAAGCT
TCGAAGGAGGGACATTTACCATACGTTGGTACGTATGTAGAAAATGGTGGTAAGATAGAC
TTAAGGTCATTTTTCGAAAGCGTTAAGTGTGGCCATGAAGATATTACAAGTATCTTTTTG
GCATTCGGATTTCCCGTAAATCAAACTTCAAGGGATAATAAAACATCCGCTTTAATGGTA
GCTGTAGGTCGTGGCCATCTTGGAACTGTTAAACTGCTATTAGAGGCAGGTGCAGATCCA
ACCAAAAGAGATAAAAAGGGTCGTACCGCTTTGTACTATGCCAAAAACAGCATAATGGGA
ATAACAAACAGTGAAGAAATTCAATTGATTGAAAATGCTATAAATAACTATTTGAAGAAG
CACTCTGAAGATAATAATGATGATGATGATGATGATGATAATAATAATGAAACATATAAA
CATGAAAAAAAGAGAGAGAAAACGCAATCACCCATATTAGCAAGCCGAAGAAGTGCCACT
CCTAGAATTGAAGACGAAGAGGACGATACGAGGATGCTCAATCTGGCAGACGATGACTTT
AATAACGATCGTGATGTCAAGGAATCTACAACTTCGGATTCGCGCAAGAGATTGGATGAC
AATGAAAATGTTGGTACTCAATACTCATTGGACTGGAAAAAACGTAAAACAAATGCCTTG
CAAGATGAAGAAAAATTAAAAAGTATCTCACCACTCTCTATGGAACCTCATTCTCCAAAG
AAGGCAAAATCTGTAGAGATAAGTAAAATACATGAAGAAACGGCTGCTGAAAGAGAAGCA
AGACTCAAGGAAGAGGAAGAATACAGAAAGAAAAGATTAGAAAAGAAAAGAAAAAAAGAA
CAGGAACTACTACAAAAGCTGGCCGAAGATGAGAAAAAAAGGATCGAAGAACAGGAGAAG
CAGAAAGTCTTAGAAATGGAAAGATTGGAAAAAGCTACTTTAGAGAAAGCAAGAAAAATG
GAAAGGGAAAAAGAAATGGAAGAAATCTCTTATAGAAGGGCAGTAAGGGACTTATATCCG
TTAGGACTGAAGATTATTAACTTCAACGATAAACTTGATTACAAAAGATTTTTGCCGCTA
TATTATTTTGTAGACGAAAAAAACGATAAATTTGTGCTCGACTTGCAAGTAATGATATTG
TTGAAGGATATCGACTTGCTCTCAAAGGACAACCAACCAACTTCTGAGAAGATTCCCGTC
GATCCCTCGCACCTGACTCCATTGTGGAATATGTTGAAATTCATTTTCCTGTATGGAGGT
AGTTATGATGATAAAAAGAACAACATGGAAAATAAAAGATATGTTGTAAACTTTGATGGG
GTTGATTTGGACACAAAGATTGGGTATGAGCTTTTGGAGTACAAAAAATTTGTTAGTTTG
CCCATGGCGTGGATTAAATGGGATAACGTTGTTATTGAAAACCACGCAAAAAGAAAAGAA
ATTGAAGGAAATATGATTCAAATATCAATAAACGAATTTGCACGATGGAGAAACGATAAG
CTGAACAAAGCGCAGCAGCCGACGCGCAAACAGCGTTCTCTAAAAATACCTCGAGAGTTA
CCGGTTAAATTTCAACACCGTATGAGCATATCCTCCGTCCTCCAGCAGACATCCAAAGAA
CCATTTTGGTAG

Protein sequence predicted based on nucleotide sequence

MNETTTKQPLKKRSLSSYLSNVSTRREELEKISKQETSEEEDTAGKHEQRETLSEEVSDK
FPENVASFRSQTTSVHQATQNNLNAKESEDLAHKNDASSHEGEVNGDSRPDDVPETNEKI
SQAIRAKISSSSSSPNVRNVDIQNHQPFSRDQLRAMLKEPKRKTVDDFIEEEGLGAVEEE
DLSDEVLEKNTTEPENVEKDIEYSDSDKDTDDVGSDDPTAPNSPIKLGRRKLVRGDQLDA
TTSSMFNNESDSELSDIDDSKNIALSSSLFRGGSSPVKETNNNLSNMNSSPAQNPKRGSV
SRSNDSNKSSHIAVSKRPKQKKGIYRDSGGRTRLQIACDKGKYDVVKKMIEEGGYDINDQ
DNAGNTALHEAALQGHIEIVELLIENGADVNIKSIEMFGDTPLIDASANGHLDVVKYLLK
NGADPTIRNAKGLTAFESVDDESEFDDEEDQKILREIKKRLSIAAKKWTNRAGIHNDKSK
NGNNAHTIDQPPFDNTTKAKNEKAADSPSMASNIDEKAPEEEFYWTDVTSRAGKEKLFKA
SKEGHLPYVGTYVENGGKIDLRSFFESVKCGHEDITSIFLAFGFPVNQTSRDNKTSALMV
AVGRGHLGTVKLLLEAGADPTKRDKKGRTALYYAKNSIMGITNSEEIQLIENAINNYLKK
HSEDNNDDDDDDDNNNETYKHEKKREKTQSPILASRRSATPRIEDEEDDTRMLNLADDDF
NNDRDVKESTTSDSRKRLDDNENVGTQYSLDWKKRKTNALQDEEKLKSISPLSMEPHSPK
KAKSVEISKIHEETAAEREARLKEEEEYRKKRLEKKRKKEQELLQKLAEDEKKRIEEQEK
QKVLEMERLEKATLEKARKMEREKEMEEISYRRAVRDLYPLGLKIINFNDKLDYKRFLPL
YYFVDEKNDKFVLDLQVMILLKDIDLLSKDNQPTSEKIPVDPSHLTPLWNMLKFIFLYGG
SYDDKKNNMENKRYVVNFDGVDLDTKIGYELLEYKKFVSLPMAWIKWDNVVIENHAKRKE
IEGNMIQISINEFARWRNDKLNKAQQPTRKQRSLKIPRELPVKFQHRMSISSVLQQTSKE
PFW*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 3252
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable