CRM1 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH202743.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
ScCD00013114
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3255nts
Open reading frame: 1 to 3255

ATGGAAGGAATTTTGGATTTTTCTAACGACTTAGATATCGCTCTATTAGATCAGGTGGTA
TCTACATTCTATCAAGGTTCAGGTGTTCAGCAAAAGCAAGCCCAAGAGATTTTGACTAAA
TTTCAAGATAATCCAGACGCTTGGCAAAAAGCTGACCAAATTCTTCAATTCTCAACTAAC
CCTCAATCAAAATTTATTGCCCTTTCCATCCTTGATAAATTAATTACTAGAAAATGGAAA
TTGTTGCCGAATGATCATAGAATTGGAATTAGGAACTTCGTTGTTGGTATGATTATCTCC
ATGTGCCAAGACGATGAAGTATTTAAAACACAAAAGAACCTGATCAATAAGTCGGACTTG
ACTTTGGTCCAAATTTTAAAGCAGGAATGGCCTCAGAATTGGCCTGAATTTATCCCAGAA
CTAATCGGCAGTTCAAGTTCCTCTGTTAATGTTTGTGAAAATAACATGATCGTTTTGAAA
CTGCTATCCGAAGAAGTCTTCGATTTCTCTGCGGAACAAATGACACAAGCTAAAGCTTTA
CATTTGAAAAATTCCATGTCAAAAGAATTTGAACAGATTTTTAAATTATGTTTTCAAGTT
TTGGAACAAGGTTCTTCAAGCTCTCTAATAGTGGCAACCTTAGAATCTTTACTGAGATAT
TTACATTGGATTCCTTATCGTTATATTTATGAAACCAATATTTTGGAATTATTAAGCACC
AAATTCATGACATCGCCTGATACAAGAGCCATCACATTGAAATGTTTGACTGAGGTTTCA
AATCTAAAAATTCCACAAGATAATGATTTGATAAAAAGACAAACTGTACTTTTTTTCCAA
AATACTCTACAACAAATTGCCACAAGTGTGATGCCCGTGACTGCCGATCTGAAAGCCACT
TATGCAAATGCTAACGGTAATGATCAATCTTTCTTACAAGATTTAGCAATGTTTCTAACT
ACATATCTCGCTCGTAATAGGGCCCTTCTAGAGAGTGATGAATCGTTAAGAGAGTTGTTG
TTGAATGCGCACCAATACTTAATTCAATTATCCAAAATCGAAGAAAGAGAACTATTTAAG
ACGACATTGGATTACTGGCATAATTTAGTAGCAGATTTGTTTTATGAGGTTCAGCGTTTG
CCTGCTACCGAAATGAGCCCATTGATACAGTTATCAGTCGGTTCACAAGCTATCTCAACT
GGATCTGGCGCCCTAAATCCGGAATATATGAAAAGATTTCCATTAAAAAAACATATTTAC
GAAGAAATTTGTTCACAGTTGAGATTGGTCATTATTGAAAATATGGTTAGACCAGAAGAA
GTCCTTGTGGTTGAAAATGATGAAGGAGAAATTGTTAGAGAGTTCGTTAAAGAATCAGAC
ACTATCCAATTATACAAATCAGAAAGAGAGGTTCTTGTATATTTGACCCATTTGAATGTT
ATCGATACAGAAGAAATCATGATCAGTAAATTGGCAAGGCAAATCGATGGTTCCGAATGG
TCATGGCATAATATCAACACTTTATCTTGGGCTATTGGTTCCATATCTGGTACAATGAGC
GAAGATACTGAAAAAAGGTTTGTAGTTACTGTTATTAAAGATTTACTAGATTTAACGGTC
AAGAAAAGGGGTAAAGATAATAAAGCTGTTGTTGCATCGGATATTATGTACGTCGTGGGC
CAATATCCTAGATTTTTGAAGGCTCACTGGAATTTTCTAAGAACAGTTATTTTGAAACTT
TTTGAATTTATGCATGAAACTCATGAGGGTGTTCAGGATATGGCATGTGACACATTCATT
AAGATTGTTCAAAAATGTAAATATCATTTTGTTATTCAACAACCCCGTGAGTCTGAGCCT
TTCATCCAAACAATAATTAGGGATATTCAAAAGACAACTGCTGACCTACAGCCGCAACAG
GTCCATACATTTTACAAGGCTTGTGGTATTATTATTTCTGAAGAAAGAAGCGTAGCGGAG
AGAAATAGATTATTAAGTGATTTGATGCAACTGCCAAATATGGCTTGGGACACTATAGTG
GAACAATCCACTGCTAACCCAACATTGTTGTTAGATTCTGAAACTGTCAAAATTATTGCG
AATATTATAAAAACCAATGTAGCTGTCTGCACCTCAATGGGAGCAGATTTCTATCCACAG
TTGGGTCACATCTATTACAATATGCTTCAGCTATACAGAGCTGTTTCTTCGATGATTTCT
GCTCAAGTTGCCGCAGAAGGTCTAATTGCTACGAAGACACCAAAGGTTCGTGGTTTGAGA
ACCATCAAAAAAGAGATTCTGAAGCTTGTTGAGACCTATATATCCAAGGCGAGAAATTTG
GATGATGTCGTTAAAGTGTTAGTTGAGCCATTATTGAACGCTGTACTTGAAGACTATATG
AACAACGTTCCTGATGCAAGGGACGCCGAAGTGCTAAATTGTATGACAACAGTAGTAGAA
AAGGTTGGTCATATGATCCCACAAGGTGTTATTTTAATTTTACAGAGTGTATTTGAATGC
ACTTTGGACATGATTAACAAAGACTTCACAGAATATCCAGAGCATCGTGTAGAATTTTAC
AAGTTATTGAAGGTCATCAATGAGAAATCATTTGCTGCATTTTTGGAACTACCTCCGGCT
GCCTTTAAATTGTTTGTTGACGCTATTTGTTGGGCGTTCAAACATAACAATAGAGATGTC
GAAGTTAATGGTCTACAGATTGCATTAGACCTAGTGAAAAACATTGAAAGAATGGGAAAT
GTCCCATTTGCAAATGAGTTCCACAAGAATTATTTTTTCATTTTTGTCAGTGAAACGTTT
TTTGTTTTAACCGATTCCGACCACAAATCCGGTTTTTCTAAGCAAGCTTTGTTACTAATG
AAGTTGATTTCTTTGGTTTATGATAACAAGATTTCGGTACCATTATATCAAGAAGCTGAG
GTACCACAAGGAACTTCAAATCAAGTCTACTTAAGCCAATATTTGGCTAATATGTTAAGT
AATGCATTCCCCCATTTAACATCCGAACAAATCGCAAGTTTTCTCTCTGCATTAACTAAA
CAATACAAAGACTTAGTGGTCTTCAAAGGCACTTTGAGGGACTTTTTGGTGCAAATCAAA
GAAGTCGGAGGTGATCCAACAGATTATTTGTTTGCTGAAGATAAAGAGAATGCATTAATG
GAACAGAATAGACTAGAGAGGGAAAAAGCTGCCAAGATTGGTGGGTTATTAAAACCTTCC
GAACTTGATGATTAG

Protein sequence predicted based on nucleotide sequence

MEGILDFSNDLDIALLDQVVSTFYQGSGVQQKQAQEILTKFQDNPDAWQKADQILQFSTN
PQSKFIALSILDKLITRKWKLLPNDHRIGIRNFVVGMIISMCQDDEVFKTQKNLINKSDL
TLVQILKQEWPQNWPEFIPELIGSSSSSVNVCENNMIVLKLLSEEVFDFSAEQMTQAKAL
HLKNSMSKEFEQIFKLCFQVLEQGSSSSLIVATLESLLRYLHWIPYRYIYETNILELLST
KFMTSPDTRAITLKCLTEVSNLKIPQDNDLIKRQTVLFFQNTLQQIATSVMPVTADLKAT
YANANGNDQSFLQDLAMFLTTYLARNRALLESDESLRELLLNAHQYLIQLSKIEERELFK
TTLDYWHNLVADLFYEVQRLPATEMSPLIQLSVGSQAISTGSGALNPEYMKRFPLKKHIY
EEICSQLRLVIIENMVRPEEVLVVENDEGEIVREFVKESDTIQLYKSEREVLVYLTHLNV
IDTEEIMISKLARQIDGSEWSWHNINTLSWAIGSISGTMSEDTEKRFVVTVIKDLLDLTV
KKRGKDNKAVVASDIMYVVGQYPRFLKAHWNFLRTVILKLFEFMHETHEGVQDMACDTFI
KIVQKCKYHFVIQQPRESEPFIQTIIRDIQKTTADLQPQQVHTFYKACGIIISEERSVAE
RNRLLSDLMQLPNMAWDTIVEQSTANPTLLLDSETVKIIANIIKTNVAVCTSMGADFYPQ
LGHIYYNMLQLYRAVSSMISAQVAAEGLIATKTPKVRGLRTIKKEILKLVETYISKARNL
DDVVKVLVEPLLNAVLEDYMNNVPDARDAEVLNCMTTVVEKVGHMIPQGVILILQSVFEC
TLDMINKDFTEYPEHRVEFYKLLKVINEKSFAAFLELPPAAFKLFVDAICWAFKHNNRDV
EVNGLQIALDLVKNIERMGNVPFANEFHKNYFFIFVSETFFVLTDSDHKSGFSKQALLLM
KLISLVYDNKISVPLYQEAEVPQGTSNQVYLSQYLANMLSNAFPHLTSEQIASFLSALTK
QYKDLVVFKGTLRDFLVQIKEVGGDPTDYLFAEDKENALMEQNRLEREKAAKIGGLLKPS
ELDD*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 3255
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable