RGA1 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH202794.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
ScCD00013132
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3024nts
Open reading frame: 1 to 3024

ATGGCATCAACTGCTCCCAATGAACAATTTCCATCCTGCGTACGATGCAAAGAATTTATT
ACCACGGGGCATGCATATGAGTTGGGTTGTGATAGATGGCACACACATTGTTTCGCTTGT
TACAAATGTGAGAAACCATTAAGCTGCGAATCTGATTTTTTAGTCCTTGGAACAGGTGCT
TTGATCTGCTTTGATTGTTCCGATTCTTGTAAAAATTGCGGTAAAAAGATTGATGATTTG
GCCATAATACTGTCCTCTTCAAATGAGGCCTATTGTTCAGATTGTTTTAAATGCTGTAAA
TGTGGTGAAAATATTGCTGACCTACGGTACGCGAAAACCAAGCGAGGTTTATTCTGTTTA
AGCTGTCACGAAAAGCTATTAGCCAAACGAAAATACTACGAAGAGAAGAAAAGGCGACTC
AAAAAGAATTTACCAAGTTTACCCACACCTGTGATTGATAATGGCCATACTGATGAGGTC
TCAGCTTCTGCAGTCCTCCCAGAAAAAACATTTAGCAGACCTGCATCACTAGTTAATGAG
ATTCCTTCAGGTTCTGAACCTTCCAAGGACATAGAAACCAATTCGAGTGATATTGTTCCG
CATTTTATCACTGGGTATAACGATAGCGATGACAACTCTGGAAGTTCAAAATTCGGTTCA
AATGTGTCCATAGATGTTATAGGACCGGAAGAAAATAGCACGGAGCATGTAAATGATGAT
GTTAAAGAGGAAGCAGAAGCACCTTCAGCGAATATGTCACTCAATGTTGCTACGGATCCA
ACCCTAAGTTGTAAAGAACCTCCTAGCCATTCGAGGAATTTGTTAAATAAAACACCGTTG
AGAAATTCTTCGGGTCAGTATCTCGCAAAATCTCCAAGCTCCTATAGACAGGGAATAATT
GTTAACGATAGTCTGGAAGAGAGCGATCAAATTGATCCTCCAAATAACAGTTCACGAAAT
GCAAGTGAATTGTTGACCTCGGTATTGCATAGCCCAGTTTCTGTTAATATGAAAAATCCG
AAGGGTTCAAATACGGATATTTTCAACACCGGTGAAATATCCCAAATGGATCCTTCGCTA
TCAAGGAAAGTATTGAATAATATTGTCGAAGAAACAAACGCACTTCAAAGGCCAGTTGTT
GAAGTTGTCAAGGAAGACAGAAGTGTTCCTGACCTAGCAGGTGTTCAGCAAGAGCAAGCA
GAGAAATATTCCTATTCAAACAATAGTGGAAAAGGCAGAAAAATTTCAAGATCATTGTCA
CGAAGGTCCAAAGATTTGATGATTAATCTAAAATCCAGAGCCACAGGCAAACAGGATAGT
AACGTTAAACTTTCACCTGCTTCTAAAGTGACATCTAGACGCTCACAAGATTTAATGAGG
GATAATGATTCACATACCGGCCTTGACACACCAAATTCTAACAGCACTTCTTTAGATATT
TTGGTGAATAATCAAAAAAGCCTAAACTATAAAAGATTTACCGATAATGGCACTCTTAGG
GTGACTAGTGGAAAGGAAACCGCCCTTGAAGAACAGAAGAATCACAGTTTTAAATCTCCG
TCACCAATTGATCATTTGTTACAATCGCCAGCTACACCAAGTAATGTATCCATGTATCGA
ACTCCGCCTTTAGATAGCTCATTGACATTTGATAGGCGGAATGGGTCTTCATACAGTAAC
CAAAATTATAGCATACCTAGTTGGCAGAAAACTCCAAAGACCCAATTGGAGAATAGCGAC
AATTTTGAGGAACAGAAGGAAACACTTTACGAAAATAGCGAATCTAGAAACGATCCCTCG
CTTGATAAAGAAATTGTCACGGCAGAGCACTATCTGAAGCAATTAAAAATAAATTTGAAA
GAACTTGAATCTCAAAGGGAAGAATTAATGAAAGAGATCACTGAAATGAAGTCTATGAAA
GAGGCTCTACGCCGACATATTGAATCCTATAATAGCGAGAAAAATAAACTATATCTTGAC
TCCAATGAATTATCAAATAATCCACCGATGATAAATGAAATCAGCTTAGGTGAGTCGCCA
CCCGTGAAACATGTTGCAACTGCAAGTTCTGTAGCGCGAAGTTCTGTTAAACCAAAATTT
TGGAAGTTTTTTTCATCTGCAAAGCCACAGACCGAGCAATCTATACAAGGTGTCAGTACA
AATAATACAAATTCCATCGTTAAAAGTGCCCCAGTTTTGCTTTCTGCTCCCTCATCTGGT
AGTAATTCCGGCCGTTTAGAAATTTCACCACCTGTGCTACAAAATCCTAATGAATTTAGT
GACGTTAGATTAGTACCTATCGAGAACGATGCAAATATGGGACAAAGTAAAGATGGAGAA
GAATATTTGGATGGAAGCAATTTGTATGGTTCAAGTCTCGTTGCTAGGTGCAATTATGAA
AACAATGAAATACCGATGATACTATCTGTCTGCATAGATTTTATTGAATCAGACGAAGAA
AATATGAGATCGGAGGGCATTTATAGAAAATCAGGTTCCCAGCTAGTCATAGAAGAAATA
GAAAAGCAATTTTCTGCATGGAAAGTACAACAAAATACCGAAACGCCAAATATTTTAACG
GAACAAGATCTCAACGTTGTTACTGGTGTGTTGAAGCGATACTTAAGAAAGCTCCCCAAC
CCTATCTTTACCTTTCAAATATACGAGCCGTTGATGAGGTTAGTTAAATCTAAAAAAATG
ATGGAAAACTTGCCATTTGTTGGAGGAAAGTTGTCCTTAGAAGCAAAGAATTCGGACACT
TATATGTCGAGCAAGAGCGCTTTAAAAAACATATTGGAAGACCTTCCAAGGGAACATTAT
AGGGTGTTAAGAGTACTGAGTGAACATATAGAAAAGGTTACACGGTACAGTCACTGGAAT
CGAATGACGCTTTATAATTTGGCTTTAGTTTTTGCTCCAGGTTTGATTCGTGATTTCAGT
GGAGAAAAGGATATTATTGATATGAAAGAAAGAAACTATATTGTCGCATTTATATTTGGG
AACTACAAAGATATCCTGACGTAG

Protein sequence predicted based on nucleotide sequence

MASTAPNEQFPSCVRCKEFITTGHAYELGCDRWHTHCFACYKCEKPLSCESDFLVLGTGA
LICFDCSDSCKNCGKKIDDLAIILSSSNEAYCSDCFKCCKCGENIADLRYAKTKRGLFCL
SCHEKLLAKRKYYEEKKRRLKKNLPSLPTPVIDNGHTDEVSASAVLPEKTFSRPASLVNE
IPSGSEPSKDIETNSSDIVPHFITGYNDSDDNSGSSKFGSNVSIDVIGPEENSTEHVNDD
VKEEAEAPSANMSLNVATDPTLSCKEPPSHSRNLLNKTPLRNSSGQYLAKSPSSYRQGII
VNDSLEESDQIDPPNNSSRNASELLTSVLHSPVSVNMKNPKGSNTDIFNTGEISQMDPSL
SRKVLNNIVEETNALQRPVVEVVKEDRSVPDLAGVQQEQAEKYSYSNNSGKGRKISRSLS
RRSKDLMINLKSRATGKQDSNVKLSPASKVTSRRSQDLMRDNDSHTGLDTPNSNSTSLDI
LVNNQKSLNYKRFTDNGTLRVTSGKETALEEQKNHSFKSPSPIDHLLQSPATPSNVSMYR
TPPLDSSLTFDRRNGSSYSNQNYSIPSWQKTPKTQLENSDNFEEQKETLYENSESRNDPS
LDKEIVTAEHYLKQLKINLKELESQREELMKEITEMKSMKEALRRHIESYNSEKNKLYLD
SNELSNNPPMINEISLGESPPVKHVATASSVARSSVKPKFWKFFSSAKPQTEQSIQGVST
NNTNSIVKSAPVLLSAPSSGSNSGRLEISPPVLQNPNEFSDVRLVPIENDANMGQSKDGE
EYLDGSNLYGSSLVARCNYENNEIPMILSVCIDFIESDEENMRSEGIYRKSGSQLVIEEI
EKQFSAWKVQQNTETPNILTEQDLNVVTGVLKRYLRKLPNPIFTFQIYEPLMRLVKSKKM
MENLPFVGGKLSLEAKNSDTYMSSKSALKNILEDLPREHYRVLRVLSEHIEKVTRYSHWN
RMTLYNLALVFAPGLIRDFSGEKDIIDMKERNYIVAFIFGNYKDILT*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 3024
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable