MTR4 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH202820.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
ScCD00013141
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3222nts
Open reading frame: 1 to 3222

ATGGATTCTACTGATCTGTTCGATGTTTTCGAGGAAACACCTGTTGAGCTTCCTACAGAT
AGTAATGGAGAAAAAAATGCTGATACAAACGTAGGAGATACTCCAGATCATACCCAGGAT
AAGAAGCATGGCCTTGAAGAAGAAAAAGAAGAGCATGAGGAAAACAACAGTGAAAATAAA
AAAATCAAATCCAATAAGAGCAAAACGGAAGATAAAAACAAAAAAGTTGTAGTACCAGTG
TTGGCAGATTCGTTCGAACAAGAAGCCTCTAGAGAAGTGGATGCATCGAAAGGACTGACA
AATTCAGAAACCTTACAAGTTGAGCAAGACGGTAAAGTCAGATTATCGCATCAGGTCCGC
CACCAGGTTGCACTACCACCGAACTATGATTATACGCCTATCGCTGAGCATAAGAGGGTA
AATGAGGCTCGTACATATCCATTCACATTAGACCCTTTCCAGGACACTGCAATCTCATGT
ATAGATAGAGGCGAATCTGTGTTGGTTTCTGCGCACACATCGGCTGGTAAAACAGTTGTG
GCTGAATATGCCATCGCACAATCTTTAAAAAATAAGCAAAGAGTCATTTATACCTCTCCA
ATTAAGGCTCTATCAAACCAAAAATACAGAGAACTTTTAGCAGAATTTGGAGATGTCGGT
TTGATGACTGGTGATATCACGATCAATCCGGATGCAGGCTGTTTGGTCATGACCACTGAG
ATTTTGAGAAGTATGTTATATAGAGGCAGTGAAGTTATGAGAGAAGTTGCCTGGGTCATC
TTTGATGAAGTGCATTACATGAGGGATAAAGAAAGAGGTGTTGTGTGGGAAGAAACGATT
ATTTTACTGCCAGATAAGGTCCGTTACGTGTTTTTATCGGCCACCATTCCAAATGCAATG
GAGTTTGCTGAATGGATATGCAAAATTCATTCTCAGCCATGTCATATTGTCTACACAAAT
TTCCGTCCAACTCCTTTACAACATTACCTGTTTCCAGCCCATGGAGATGGTATTTATCTG
GTGGTTGACGAAAAAAGTACCTTCAGAGAGGAAAATTTCCAAAAAGCAATGGCGTCCATA
AGTAACCAGATAGGTGATGATCCAAATTCCACTGATTCAAGAGGTAAAAAGGGTCAAACC
TATAAAGGTGGCTCCGCTAAGGGTGACGCAAAAGGTGACATTTATAAGATAGTGAAAATG
ATTTGGAAGAAAAAGTACAATCCAGTGATTGTATTTTCGTTCAGTAAACGTGATTGTGAA
GAATTAGCATTGAAGATGTCTAAATTAGATTTTAATTCTGATGATGAAAAGGAGGCTTTG
ACGAAGATTTTTAACAATGCCATTGCACTATTACCAGAGACAGACAGAGAGCTACCCCAA
ATTAAACACATTTTACCATTATTAAGAAGAGGTATCGGTATTCATCATTCCGGTTTACTG
CCCATTTTGAAGGAAGTCATTGAAATTCTGTTTCAAGAGGGATTTTTGAAGGTGTTGTTT
GCAACAGAAACATTTTCGATTGGATTAAACATGCCTGCTAAAACTGTTGTCTTTACATCG
GTCAGAAAGTGGGATGGTCAACAATTCCGCTGGGTTTCAGGTGGAGAATATATACAAATG
TCTGGTCGTGCAGGTCGTCGTGGTTTAGATGATCGTGGTATTGTTATTATGATGATTGAT
GAAAAAATGGAACCTCAAGTTGCTAAAGGAATGGTTAAGGGCCAAGCAGATAGGCTAGAC
TCGGCTTTTCACTTAGGATATAATATGATTTTGAACTTAATGAGAGTTGAAGGTATCTCT
CCGGAGTTTATGTTGGAGCATTCTTTCTTCCAATTTCAAAACGTTATTTCAGTACCAGTT
ATGGAAAAGAAACTTGCTGAACTGAAAAAAGACTTTGATGGCATCGAAGTCGAAGATGAA
GAAAATGTTAAAGAATACCATGAGATTGAGCAGGCTATCAAAGGTTACCGTGAAGATGTT
CGTCAAGTTGTCACCCATCCGGCAAACGCCTTAAGTTTCCTACAACCAGGCAGATTAGTT
GAAATCTCTGTCAATGGTAAGGATAACTATGGCTGGGGTGCTGTTGTCGATTTTGCTAAA
AGAATTAACAAACGCAACCCAAGTGCTGTCTATACAGACCATGAATCCTATATTGTCAAT
GTGGTCGTCAATACCATGTATATAGACTCTCCAGTCAACTTGTTGAAACCTTTCAACCCT
ACCTTACCAGAAGGGATTCGCCCAGCGGAAGAAGGTGAAAAAAGCATATGTGCTGTTATT
CCCATAACTTTGGATTCAATCAAGTCGATTGGTAATTTGAGACTATATATGCCCAAAGAT
ATTAGAGCCAGCGGTCAAAAAGAAACTGTTGGGAAGTCTTTAAGGGAGGTTAATCGGAGG
TTCCCGGATGGTATTCCCGTGCTGGATCCTGTTAAAAACATGAAGATCGAAGATGAGGAC
TTTTTAAAGCTAATGAAGAAGATTGACGTTTTAAACACAAAGTTATCCTCCAATCCCTTA
ACCAATTCCATGAGACTAGAAGAACTATATGGTAAATATAGTAGAAAACACGATTTACAT
GAAGATATGAAACAATTGAAACGCAAAATTTCAGAATCACAAGCCGTAATCCAACTGGAC
GATCTTCGTCGCCGTAAAAGAGTTTTGCGCCGTTTAGGATTTTGTACTCCTAATGACATT
ATTGAACTGAAAGGTAGAGTTGCATGTGAAATATCTAGTGGTGATGAACTGTTACTAACA
GAATTGATCTTCAATGGTAATTTCAATGAGTTGAAAACGGAACAAGCAGCAGCATTATTA
TCATGCTTTGCATTCCAAGAACGCTGTAAAGAAGCGCCTAGATTGAAACCAGAGCTTGCC
GAACCTTTGAAGGCTATGAGAGAAATTGCAGCAAAGATCGCTAAGATAATGAAGGATTCT
AAAATTGAAGTTGTAGAAAAGGACTACGTTGAAAGCTTCAGACATGAACTAATGGAAGTT
GTTTACGAATGGTGTAGAGGAGCTACTTTTACGCAAATCTGTAAAATGACCGACGTTTAC
GAAGGTTCGTTGATCAGAATGTTCAAGAGATTAGAGGAATTGGTGAAGGAGCTGGTAGAC
GTCGCCAATACCATTGGTAACTCTTCACTTAAGGAGAAGATGGAAGCTGTCTTGAAATTA
ATTCATAGAGATATCGTATCTGCGGGTTCTTTGTATTTATAG

Protein sequence predicted based on nucleotide sequence

MDSTDLFDVFEETPVELPTDSNGEKNADTNVGDTPDHTQDKKHGLEEEKEEHEENNSENK
KIKSNKSKTEDKNKKVVVPVLADSFEQEASREVDASKGLTNSETLQVEQDGKVRLSHQVR
HQVALPPNYDYTPIAEHKRVNEARTYPFTLDPFQDTAISCIDRGESVLVSAHTSAGKTVV
AEYAIAQSLKNKQRVIYTSPIKALSNQKYRELLAEFGDVGLMTGDITINPDAGCLVMTTE
ILRSMLYRGSEVMREVAWVIFDEVHYMRDKERGVVWEETIILLPDKVRYVFLSATIPNAM
EFAEWICKIHSQPCHIVYTNFRPTPLQHYLFPAHGDGIYLVVDEKSTFREENFQKAMASI
SNQIGDDPNSTDSRGKKGQTYKGGSAKGDAKGDIYKIVKMIWKKKYNPVIVFSFSKRDCE
ELALKMSKLDFNSDDEKEALTKIFNNAIALLPETDRELPQIKHILPLLRRGIGIHHSGLL
PILKEVIEILFQEGFLKVLFATETFSIGLNMPAKTVVFTSVRKWDGQQFRWVSGGEYIQM
SGRAGRRGLDDRGIVIMMIDEKMEPQVAKGMVKGQADRLDSAFHLGYNMILNLMRVEGIS
PEFMLEHSFFQFQNVISVPVMEKKLAELKKDFDGIEVEDEENVKEYHEIEQAIKGYREDV
RQVVTHPANALSFLQPGRLVEISVNGKDNYGWGAVVDFAKRINKRNPSAVYTDHESYIVN
VVVNTMYIDSPVNLLKPFNPTLPEGIRPAEEGEKSICAVIPITLDSIKSIGNLRLYMPKD
IRASGQKETVGKSLREVNRRFPDGIPVLDPVKNMKIEDEDFLKLMKKIDVLNTKLSSNPL
TNSMRLEELYGKYSRKHDLHEDMKQLKRKISESQAVIQLDDLRRRKRVLRRLGFCTPNDI
IELKGRVACEISSGDELLLTELIFNGNFNELKTEQAAALLSCFAFQERCKEAPRLKPELA
EPLKAMREIAAKIAKIMKDSKIEVVEKDYVESFRHELMEVVYEWCRGATFTQICKMTDVY
EGSLIRMFKRLEELVKELVDVANTIGNSSLKEKMEAVLKLIHRDIVSAGSLYL*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 3222
Mutations:
None
Discrepancy:
c2797a,P933T; t3204g
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable