AMS1 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH202830.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
ScCD00013145
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3252nts
Open reading frame: 1 to 3252

ATGTCATCTGAGGATATCATTTATGATCCTCAGTTCAAACCTGTTCAGGGAATCTACGAG
AATAGATTAAGACAATTTATTGATACTGGTGGGGATTACCATGACTTAAATTTACCCAAG
TTTTATGATAAGAAGCGCATTTCATTAGATCATGATCACGTTAAGGTTTGGTGGTACCAG
GTTTCTTTTGAACGTGGATCTTCCCCTGTTTCCCCTGATAAGAGGCCATCTTGGAAATCT
ATTATTGAACGTGACAAGAAGGGTGAATTGGAGTTTAGAGAAGCGAACATAAATCAGCCC
TTTGGACCCAGTTGGTCTACCACTTGGTTCAAGGTGAAAATCTCTTTGCCTGAAGATTGG
GTTAAATCAAACGAGCAACTACTTTTCCAATGGGATTGTTCTAACGAAGGAATTGTCATA
GACCCAAAGACTCTAATCCCTGTGACTGCTTTTTCAGGTGGTGAAAGGACTGAATATGTT
TTACCGAAAACATCGGATGGGAAACATTTCTTTTACATCGAAGCTGGTAATAATGGTATG
TTTGGCTGCGGTGCAGGATCCACTATAAATCCACCAGATGATAATAGATTTTTTCATTTG
AGAAAAGCTGATATCGTTTGGCCCGATCTAGATGCTCGTGCATTGTATATCGATTTTTGG
ATGCTAGGGGATGCTGCAAGAGAGCTACCTGGAGACTCCTGGCAAAAACACCAGGCAAGA
CAACTAGGCAATGCAGTTATGAATCTTTTTGACCCGAATGATCGTTCAAGTGTTCGTAAA
TGTCGTGAATTACTACAAAGGGAATACTTTGACTCCTTTTTAGAAAGCAGTAAGGTTTAC
GAACAAGGTGAATCTCAAGTTTTAACAAATGTTTATGGTATCGGCAACTGTCATATAGAT
ACAGCTTGGCTATGGCCATTCGCAGAAACAAGAAGGAAAATTGTGAGATCTTGGTCTTCC
CAATGTACTTTAATGGATCGCTTCCCAGAGTATAAATTTGTTGCTTCACAAGCCCAACAA
TTTAAATGGTTATTAGAAGATCATCCCGAATTTTTCAATAAAGTGTTAATTCCAAAAATT
CAGCAATCTCAGTTTTTTGCTGTTGGTGGTACATGGGTTGAGAATGATACCAATATTCCC
TCAGGGGAATCGCTGGCAAGGCAATTCTTTTTTGGTCAAAGGTTTTTCTTGAAGCATTTT
GGTCTCAAGTCAAAGATATTTTGGTTACCTGACACCTTTGGTTATTCTTCGCAAATGCCA
CAACTTTGCCGCTTATCTGGTATTGATAAATTTTTGACTCAAAAGCTTTCCTGGAATAAC
ATCAACAGTTTTCCGCATAGCACGTTTAACTGGGCAGGGATTGACGGTTCTCAATTATTG
ACTCACATGCCTCCTGGTAATACGTATACAGCAGATTCACACTTCGGTGATGTTTTGCGT
ACTGCCAAGCAGAATAAGACACCAGAATATTACGGCTCCGGTTTAATGTTGTACGGAAAG
GGTGATGGTGGTGGAGGACCAACTGAGGAAATGCTGCAAAAAATGAGGCGTATTAGATCC
ATGAATAACAGAAATGGTAATGTTATTCCAAAGTTACAAGTTGGTATAACGGTTGATGAA
TTTTATGATGACATTTTGAAAAGGACTAACCAAGGCCATGATTTACCCACATGGAGTGGT
GAATTGTATTTCGAATTTCATAGGGGGACATACACCAGTCAAGCTCAGACCAAAAAATTA
ATGAGGTTGTCCGAAATTAAACTACATGATTTGGAATGGATAGCTGCCAAAACATCAGTT
TTATATCCCGATTCGTATAAATACCCTTCCAAACAGATTAACGAACTTTGGGAGAATGTT
TTGTTATGTCAATTTCACGATGTTCTACCAGGATCATGTATTGAAATGGTATATAAGTAT
GAGGCTGTCCCTATGTTGCACAACGTTGTGAAAGAATGTACTTCCTTAATAGACAAAACC
GTTCAATTTCTTCAGAGTCAGAGTAAGGCAGATTTGGTTGAGATGAGAACATTAACTTGG
TCAAAACCAGAAAAGGTTTCTGAGGAGTGCTCGTTGAACGGTAGTTACACGTCCTCTGTA
ACCGGGTACGATGACTATATAGTCCTTGCCAATGGGAAGTTGAAAGTTATCATTTGCAAA
AAGACTGGGGTGATCACAAGCATTACGGATGAAACTTTGGGGGTAGAATACCTGGACACG
GAACATGGCAGAAACAAATTGGGCGCCAACCAGTTTGTTATTTATGATGATAAACCTTTG
GGCTGGCAGGCTTGGGATACTGAACTTTATTCCGTCAACCAATACAAGTATGTAACAAAG
CCGAAGAAAGTTCAAGTTTCCTGCAACACAAAAGAGAAATGTGCTGTTGAGGTCATTTTT
CAAATTTCCGAAAAATGCAAAATCAAATCAGTGATATCACTCAATGCAACTGCGGTCACT
GATGCTAAATTAAGTAAAGTAGACATTTCTACGACGGTAGAAAACTGGGATGCTAGAAAC
AAATTTTTAAAAGTTGAATTTCCCGTTAATATTCGGAACGATTTTGCTTCGTATGAAACT
CAATTCGGGATTACCAAAAGACCAACACATTATAATACCTCATGGGACGTCGCTAAATTT
GAGGTTTGCCATCACAAATTTGCTGATTATTCTGAATACAGCAAGGGTGTTTCAATTTTG
AACGATTGCAAATATGGGTTTTCCACACACGGTAACTTGATGAGATTATCATTATTAAGG
TCACCAAAGGCTCCGGATGCGCATGCTGATATGGGTACTCATGAGATAAAATATGCTATC
TATCCACATAGAGGAGCACTATCAAGCGACACTGTTAAGCTCGCTCACGAATTCAATTAT
TGCTTCAAATACAAACTCCCCAAAGATATTGGCATGAATTTTGATGACATCATAAGCATT
TCCGGAGATGAGAATGTCATTTTGTCTAACATAAAAAGAGGCGAAGATGATAGTGCTGTC
AAGTCCAACTATTCATTAAATCCAAGGGATGAACAAAGTATTGTGGTAAGGGTTTACGAA
TCTCTTGGCGGGGAATCTTTTGCTTCACTAAATACCACACTAAATTTGAAGCGTATTGAG
AAGGTCGATAACTTGGAGATGAAAGTTTATAAGAGTTTGACAGCAACGCGAGACGAATCA
AATCATGCAATAAACAGAATTCCCATTAAATTGAGACCTTTTGAGATTGCCTCATTCAGG
TTGTATTTCTAG

Protein sequence predicted based on nucleotide sequence

MSSEDIIYDPQFKPVQGIYENRLRQFIDTGGDYHDLNLPKFYDKKRISLDHDHVKVWWYQ
VSFERGSSPVSPDKRPSWKSIIERDKKGELEFREANINQPFGPSWSTTWFKVKISLPEDW
VKSNEQLLFQWDCSNEGIVIDPKTLIPVTAFSGGERTEYVLPKTSDGKHFFYIEAGNNGM
FGCGAGSTINPPDDNRFFHLRKADIVWPDLDARALYIDFWMLGDAARELPGDSWQKHQAR
QLGNAVMNLFDPNDRSSVRKCRELLQREYFDSFLESSKVYEQGESQVLTNVYGIGNCHID
TAWLWPFAETRRKIVRSWSSQCTLMDRFPEYKFVASQAQQFKWLLEDHPEFFNKVLIPKI
QQSQFFAVGGTWVENDTNIPSGESLARQFFFGQRFFLKHFGLKSKIFWLPDTFGYSSQMP
QLCRLSGIDKFLTQKLSWNNINSFPHSTFNWAGIDGSQLLTHMPPGNTYTADSHFGDVLR
TAKQNKTPEYYGSGLMLYGKGDGGGGPTEEMLQKMRRIRSMNNRNGNVIPKLQVGITVDE
FYDDILKRTNQGHDLPTWSGELYFEFHRGTYTSQAQTKKLMRLSEIKLHDLEWIAAKTSV
LYPDSYKYPSKQINELWENVLLCQFHDVLPGSCIEMVYKYEAVPMLHNVVKECTSLIDKT
VQFLQSQSKADLVEMRTLTWSKPEKVSEECSLNGSYTSSVTGYDDYIVLANGKLKVIICK
KTGVITSITDETLGVEYLDTEHGRNKLGANQFVIYDDKPLGWQAWDTELYSVNQYKYVTK
PKKVQVSCNTKEKCAVEVIFQISEKCKIKSVISLNATAVTDAKLSKVDISTTVENWDARN
KFLKVEFPVNIRNDFASYETQFGITKRPTHYNTSWDVAKFEVCHHKFADYSEYSKGVSIL
NDCKYGFSTHGNLMRLSLLRSPKAPDAHADMGTHEIKYAIYPHRGALSSDTVKLAHEFNY
CFKYKLPKDIGMNFDDIISISGDENVILSNIKRGEDDSAVKSNYSLNPRDEQSIVVRVYE
SLGGESFASLNTTLNLKRIEKVDNLEMKVYKSLTATRDESNHAINRIPIKLRPFEIASFR
LYF*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 3252
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable