KIP1 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH202838.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
ScCD00013151
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3336nts
Open reading frame: 1 to 3336

ATGGCTCGTTCTTCCTTGCCCAACCGCCGCACCGCCCAGTTCGAAGCGAACAAGAGGAGG
ACCATTGCACATGCTCCATCTCCAAGTCTTTCAAATGGGATGCACACTCTAACGCCGCCC
ACCTGTAACAATGGTGCTGCCACTTCAGACTCCAATATACATGTATATGTAAGGTGCAGA
TCGCGTAATAAGCGAGAAATAGAGGAAAAAAGTAGTGTAGTTATATCTACACTAGGCCCA
CAAGGGAAAGAAATCATTCTGTCCAACGGTTCTCACCAATCGTATTCGTCCTCGAAGAAA
ACTTACCAATTTGATCAGGTGTTCGGCGCAGAATCTGACCAGGAAACAGTGTTTAATGCC
ACTGCAAAAAACTACATTAAGGAAATGTTGCACGGGTACAATTGTACAATATTTGCATAC
GGTCAAACGGGAACAGGTAAAACCTACACTATGTCTGGCGATATAAATATTCTCGGTGAT
GTGCAATCTACCGATAATCTATTATTAGGAGAGCATGCAGGTATCATACCACGGGTTCTG
GTCGATTTGTTTAAAGAATTGAGCTCCTTAAATAAAGAGTACTCCGTAAAAATATCCTTT
TTAGAGTTGTACAATGAAAATTTGAAAGATCTGCTCTCTGATAGTGAGGACGATGATCCT
GCAGTCAACGATCCCCAGAGGCAGATTCGTATTTTTGACAATAACAACAATAATTCATCC
ATCATGGTCAAGGGGATGCAGGAAATCTTTATTAACTCTGCACACGAAGGCTTGAATTTG
CTAATGCAGGGTTCGTTAAAAAGGAAAGTGGCCGCTACTAAATGCAACGATCTTTCATCA
AGGTCTCACACCGTCTTTACAATCACAACAAACATAGTTGAGCAAGATAGCAAAGACCAT
GGACAAAACAAAAATTTTGTTAAAATTGGCAAATTGAATTTGGTGGATTTGGCAGGCAGT
GAAAACATCAACAGATCGGGTGCGGAGAATAAAAGGGCTCAAGAAGCTGGCCTAATAAAC
AAATCGCTGCTAACACTAGGCCGTGTTATCAACGCACTCGTTGATCATTCTAACCATATA
CCTTACAGAGAATCTAAGCTAACAAGATTGCTACAAGACTCTTTAGGTGGTATGACGAAA
ACATGCATTATCGCAACTATATCACCTGCGAAAATATCCATGGAAGAGACTGCAAGTACG
CTAGAATATGCAACGAGAGCCAAATCAATTAAGAATACTCCACAAGTAAATCAGTCTTTA
TCGAAGGATACATGTCTCAAAGACTACATTCAAGAGATTGAAAAATTAAGAAATGATTTG
AAAAATTCAAGAAACAAACAAGGTATATTTATAACTCAAGATCAGTTGGACCTTTACGAG
AGCAATTCTATCTTGATTGATGAGCAAAATCTAAAAATACATAACCTGCGAGAACAAATT
AAAAAATTCAAAGAAAACTACCTGAACCAATTAGATATCAATAATCTTTTACAGTCTGAA
AAGGAAAAACTAATTGCCATAATACAGAATTTTAATGTCGATTTTTCTAACTTTTACTCG
GAAATCCAAAAAATTCACCATACTAATCTCGAACTAATGAATGAAGTCATACAACAGAGA
GATTTTTCACTAGAAAATTCTCAAAAACAGTATAATACGAACCAGAACATGCAATTAAAA
ATCTCTCAACAAGTTTTACAGACTTTGAACACTTTACAGGGCTCTTTAAATAATTATAAC
TCTAAATGTTCCGAAGTTATCAAAGGCGTCACCGAAGAACTAACCAGGAACGTAAATACC
CATAAGGCGAAACACGATTCTACTCTCAAATCGTTATTAAACATTACTACTAACTTATTG
ATGAATCAGATGAACGAACTGGTGCGTAGTATTTCGACTTCATTGGAAATATTTCAGAGT
GATTCTACTTCTCACTATCGTAAAGATTTGAATGAAATCTACCAATCACATCAACAATTT
CTAAAAAATTTACAAAACGATATTAAAAGCTGTCTTGATTCGATAGGCAGTTCAATTCTA
ACTTCCATAAACGAAATATCGCAAAATTGCACCACTAACTTGAATAGTATGAATGTTTTA
ATAGAAAACCAGCAGTCAGGATCATCGAAATTAATTAAAGAGCAAGATTTAGAAATAAAA
AAACTGAAAAACGATCTGATCAATGAGCGCAGGATTTCTAACCAATTCAACCAACAGTTG
GCTGAAATGAAGCGATATTTTCAGGATCACGTTTCCAGGACGCGTAGTGAATTCCACGAC
GAACTTAACAAATGTATCGATAACCTAAAAGATAAACAATCTAAGTTGGATCAAGATATC
TGGCAGAAGACGGCCTCTATTTTCAACGAAACAGATATCGTAGTTAATAAAATTCATTCC
GACTCAATAGCATCCCTCGCTCATAATGCTGAAAACACTTTGAAAACGGTTTCTCAGAAC
AATGAAAGCTTTACTAACGATTTAATCAGTCTATCACGCGGAATGAACATGGACATATCC
TCCAAACTGAGAAGTTTGCCCATCAATGAATTTTTAAACAAGATATCACAAACCATTTGT
GAAACCTGTGGCGATGATAACACAATCGCATCAAATCCAGTATTGACCTCTATTAAAAAA
TTTCAAAATATAATTTGTTCAGACATTGCCCTAACAAATGAGAAGATCATGTCATTAATA
GATGAAATACAATCACAAATTGAAACCATATCTAATGAAAACAATATCAATTTGATTGCA
ATAAATGAAAATTTTAATTCTTTGTGCAATTTTATATTAACTGATTACGATGAGAATATT
ATGCAAATCTCAAAAACACAAGATGAGGTGCTTTCTGAACATTGCGAGAAGCTACAATCA
CTGAAAATACTGGGTATGGACATTTTCACTGCTCACAGCATAGAAAAACCCCTTCATGAG
CATACAAGACCTGAAGCGTCAGTAATCAAGGCTTTACCCTTATTGGATTATCCAAAACAA
TTTCAGATTTATAGGGATGCTGAAAATAAGAGCAAAGACGACACATCTAATTCTCGTACT
TGTATACCAAACTTGTCAACTAATGAAAATTTTCCTCTTTCACAATTCAGTCCAAAAACC
CCAGTGCCAGTGCCTGATCAACCTCTACCAAAAGTTCTTATACCGAAAAGCATAAACTCG
GCCAAGTCCAATAGATCAAAGACCTTACCAAATACAGAGGGTACTGGACGAGAATCGCAG
AACAATTTGAAGAGAAGATTTACCACCGAGCCAATATTGAAGGGAGAAGAAACTGAAAAT
AATGACATACTGCAAAATAAAAAACTTCATCAATAG

Protein sequence predicted based on nucleotide sequence

MARSSLPNRRTAQFEANKRRTIAHAPSPSLSNGMHTLTPPTCNNGAATSDSNIHVYVRCR
SRNKREIEEKSSVVISTLGPQGKEIILSNGSHQSYSSSKKTYQFDQVFGAESDQETVFNA
TAKNYIKEMLHGYNCTIFAYGQTGTGKTYTMSGDINILGDVQSTDNLLLGEHAGIIPRVL
VDLFKELSSLNKEYSVKISFLELYNENLKDLLSDSEDDDPAVNDPQRQIRIFDNNNNNSS
IMVKGMQEIFINSAHEGLNLLMQGSLKRKVAATKCNDLSSRSHTVFTITTNIVEQDSKDH
GQNKNFVKIGKLNLVDLAGSENINRSGAENKRAQEAGLINKSLLTLGRVINALVDHSNHI
PYRESKLTRLLQDSLGGMTKTCIIATISPAKISMEETASTLEYATRAKSIKNTPQVNQSL
SKDTCLKDYIQEIEKLRNDLKNSRNKQGIFITQDQLDLYESNSILIDEQNLKIHNLREQI
KKFKENYLNQLDINNLLQSEKEKLIAIIQNFNVDFSNFYSEIQKIHHTNLELMNEVIQQR
DFSLENSQKQYNTNQNMQLKISQQVLQTLNTLQGSLNNYNSKCSEVIKGVTEELTRNVNT
HKAKHDSTLKSLLNITTNLLMNQMNELVRSISTSLEIFQSDSTSHYRKDLNEIYQSHQQF
LKNLQNDIKSCLDSIGSSILTSINEISQNCTTNLNSMNVLIENQQSGSSKLIKEQDLEIK
KLKNDLINERRISNQFNQQLAEMKRYFQDHVSRTRSEFHDELNKCIDNLKDKQSKLDQDI
WQKTASIFNETDIVVNKIHSDSIASLAHNAENTLKTVSQNNESFTNDLISLSRGMNMDIS
SKLRSLPINEFLNKISQTICETCGDDNTIASNPVLTSIKKFQNIICSDIALTNEKIMSLI
DEIQSQIETISNENNINLIAINENFNSLCNFILTDYDENIMQISKTQDEVLSEHCEKLQS
LKILGMDIFTAHSIEKPLHEHTRPEASVIKALPLLDYPKQFQIYRDAENKSKDDTSNSRT
CIPNLSTNENFPLSQFSPKTPVPVPDQPLPKVLIPKSINSAKSNRSKTLPNTEGTGRESQ
NNLKRRFTTEPILKGEETENNDILQNKKLHQ*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 3336
Mutations:
None
Discrepancy:
a676c,K226Q
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable