PHO81 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH202858.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
ScCD00013160
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3537nts
Open reading frame: 1 to 3537

ATGAAATTCGGCAAGTATTTGGAAGCCAGGCAGTTGGAACTGGCGGAGTACAACAGCCAT
TTTATTGATTATAAGGCTCTAAAGAAACTTATCAAACAATTGGCCATTCCCACGCTAAAG
GCTAGTTCAGATTTAGATTTGCATTTAACATTAGATGACATCGACGAAAAAATTATACAT
CAGAGACTGCAAGAAAATAAAGCTGCCTTTTTTTTTAAACTAGAGAGGGAGCTGGAAAAA
GTAAATGGTTATTATTTGGCGAGAGAGTCCGATTTAAGAATAAAATTCAATATATTACAC
TCAAAATATAAAGATTATAAGATAAATGGTAAGCTAAACTCAAACCAAGCTACCTCATTT
AAAAACTTATATGCGGCTTTCAAAAAGTTTCAAAAGGATCTTAGAAATTTGGAGCAGTAT
GTTGAGTTAAATAAAACGGGATTTTCAAAAGCTCTGAAGAAATGGGACAAGAGATCTCAA
TCTCACGATAAAGATTTTTACCTTGCTACTGTTGTTTCCATTCAACCAATTTTTACCAGA
GATGGGCCACTAAAATTAAACGATGAAACTTTACATATTCTCTTAGAGCTGAATGATATT
GACAACAACAATAGAAGGGCAGACATACAATCCAGTACGTTTACTAATGACGATGATGAC
GACAATAATACCAGCAACAATAATAAACACAATAATAATAATAACAATAATAATAACAAC
AACAACAACAACAATAATAATAACATATTACACAATAATTATGAATTAACAACATCAAAG
ATTTCGGAAAACCAACTTGAACATCTTTTTCAAGCATCTTCCTCCTCCTTGGACATGGAG
ATGGAAATCGAAAACTGGTATAAGGAAATTTTGAATATAGCCACGGTGAAAGATGTTCAA
AGAAAGCATGCACTACTAAGAAACTTCAGAGAAACCAAGATATTCACATATCTGCTACAA
AATTCCTCAGAATCTTTTCATAAAAATGTCTTTTCTTTGTTGAAGGAGTGCTTAACGACG
CTGTTTTTATTACTGGTGGCTAGTCCTTTGGACGATAACTCTCTGCATATTTTCTATAAA
AGCAATCAGGATCATATTGATCTGTCATATTGTGACGAAGATGATCAAGTATTTTCACGA
AAAAACGTATTTCACGAGGCTGCAAGTTGTCCAGAAAAATCACGCTTATTTATTCTAGAT
GAGGCTTTAACAACATCAAAGTTATCTAAAGAAACAGTACAAAAACTACTGAATGCCCAA
GATATACATTCGCGGGTGCCGCTACATTATGCTGCAGAGCTAGGAAAACTGGAATTTGTT
CACTCGTTGCTAATCACCAATCTTTTAGAGGACGTTGATCCCATTGATAGTGATTCTAAG
ACACCCTTGGTTTTGGCTATCACTAACAACCACATAGATGTTGTTAGGGATCTGCTGACT
ATTGGTGGTGCGAACGCATCACCCATTGAAAAGCCAATTCTGGATTATAGTAAGAATGTA
ATCAGCTCTACTAAAGTCCAGTTCGATCCTTTAAATGTTGCTTGTAAATTTAATAATCAT
GACGCCGCCAAGTTACTTTTAGAAATTCGGAGTAAGCAAAATGCTGATAATGCCAAGAAC
AAAAGTTCACAACACTTGTGTCAACCGCTTTTCAAAAAGAACTCAACAGGTTTATGCACT
CTTCATATAGTGGCAAAAATTGGCGGCGATCCACAATTAATTCAACTATTGATACGTTAT
GGGGCGGATCCCAACGAAATTGATGGGTTTAATAAATGGACGCCCATTTTTTACGCTGTT
CGTTCAGGTCATTCTGAAGTTATTACTGAACTATTGAAACATAATGCACGTCTGGATATT
GAAGATGACAACGGCCATTCGCCACTTTTTTACGCCTTATGGGAGAGCCACGTTGATGTT
TTGAATGCACTTTTACAAAGACCATTAAATTTGCCATCTGCACCCCTGAATGAAATAAAT
TCGCAGTCTAGTACGCAACGCCTTAATACAATAGATTTAACCCCAAATGATGACAAATTT
GATTTAGACATTCAAGATAGCATTCCGGATTTTGCTTTACCACCGCCAATCATTCCACTA
AGGAAATATGGTCATAATTTTTTGGAGAAAAAAATTTTCATCAAATTAAAGTTGAGGCCA
GGTCTCGAGTCTATCAAGTTGACTCAGGATAACGGCATTATTATGTCATCATCACCAGGC
AGAATTACTTTATCCTCAAACTTACCTGAAATAATACCGCGAAATGTTATTTTACCTGTT
AGATCTGGCGAAATTAATAACTTTTGTAAGGATATCAGTGAAACAAATGATGAAGAAGAT
GATGATGAAATTAGTGAAGATCATGATGATGGAGAGATAATTTTCCAAGTAGATTCAATC
GACGATTTTTCAATGGATTTCGAGATATTTCCTTCATTTGGCACAAGGATAATTGCCAAA
ACAACGGCAATGCCATTCCTTTTCAAGAAGGTGGCAATAAATAGTATTGCAACCATGAAT
TTACCCTTATTCGACACAAGACTAAATAATATTGGATCTCTCACTTTGGATTACCAAATT
ATTTTTCCTTATCCGGGAAATCCACTGAAAATCATAAATTATGAGCCGTATTGGAAATCT
ACAGGAAGTGATTTAATGACCTCTAGTAAAGACGGTAATTTTGTTACTTCTTCATCGTTG
AATGGCAGTTTTATTAGTGTATTAGTTTGCGCTCTGAATGATGAAACCATAGTGGCTGCG
CCAAAACCATACGTTGAATTCAAAGGCACGAAGATTCTGTTAAATGATTTAACGAAAGAA
CAATTGGAAAAGGTAGTGGATTATGACTTCGGTAAAATTGATGGAAGCTTCGATGAAGTA
ACATTGAAACAATACTTATCTTCGAGAGTGGTGCCTCTCAGAAGCCTACTAGAAGTTATT
CCCGGGTCAGCCCAGCTGGTAATTCGTGTATATTTTCCTACAGATAAGGAAATTGACACA
ATTCCCATCAAAATATCGCCATTTATAAATATCAATCAATTCATTGATAAGCTTTTACTA
ATCATTTTCGAGCATGAACGTTTTTTGCGTCACAGCGGAAGTGGGAGTATGCGCCAAATA
GTTTTCAGTTCATGCAATTGGGAAGCTTGCTCGATCCTTAACTGGAAACAACCCAATTTT
CCGGTTTTGTTGCAAATGAAAAATCTACTCAGAGATTCAACCACAGGCAAATTTGTAGGT
GATACTCCTAATTGTTTAAAGGAACTGGCCGTAAATCCTCAAAAAATGTCGTATTTAAAC
ACGGAACTAATAAACATACATACAATGGTTCAATTTGCTATGAACAATAATTTGTTAGGT
GTAACGCTTCCATATGAAGTACTGAAGATATGCCCGTCGTTGGCTAGGATCATCAAACAA
AACGGACTACTGTTGATTGCATCGGTCGGGGAAAATGACCAAATACCAGCTGATGGAGGT
TACAGCGGGATCTACTACGCTTGTGAGTTGCTTTTTGAGAATAATATTGATATGTAG

Protein sequence predicted based on nucleotide sequence

MKFGKYLEARQLELAEYNSHFIDYKALKKLIKQLAIPTLKASSDLDLHLTLDDIDEKIIH
QRLQENKAAFFFKLERELEKVNGYYLARESDLRIKFNILHSKYKDYKINGKLNSNQATSF
KNLYAAFKKFQKDLRNLEQYVELNKTGFSKALKKWDKRSQSHDKDFYLATVVSIQPIFTR
DGPLKLNDETLHILLELNDIDNNNRRADIQSSTFTNDDDDDNNTSNNNKHNNNNNNNNNN
NNNNNNNNILHNNYELTTSKISENQLEHLFQASSSSLDMEMEIENWYKEILNIATVKDVQ
RKHALLRNFRETKIFTYLLQNSSESFHKNVFSLLKECLTTLFLLLVASPLDDNSLHIFYK
SNQDHIDLSYCDEDDQVFSRKNVFHEAASCPEKSRLFILDEALTTSKLSKETVQKLLNAQ
DIHSRVPLHYAAELGKLEFVHSLLITNLLEDVDPIDSDSKTPLVLAITNNHIDVVRDLLT
IGGANASPIEKPILDYSKNVISSTKVQFDPLNVACKFNNHDAAKLLLEIRSKQNADNAKN
KSSQHLCQPLFKKNSTGLCTLHIVAKIGGDPQLIQLLIRYGADPNEIDGFNKWTPIFYAV
RSGHSEVITELLKHNARLDIEDDNGHSPLFYALWESHVDVLNALLQRPLNLPSAPLNEIN
SQSSTQRLNTIDLTPNDDKFDLDIQDSIPDFALPPPIIPLRKYGHNFLEKKIFIKLKLRP
GLESIKLTQDNGIIMSSSPGRITLSSNLPEIIPRNVILPVRSGEINNFCKDISETNDEED
DDEISEDHDDGEIIFQVDSIDDFSMDFEIFPSFGTRIIAKTTAMPFLFKKVAINSIATMN
LPLFDTRLNNIGSLTLDYQIIFPYPGNPLKIINYEPYWKSTGSDLMTSSKDGNFVTSSSL
NGSFISVLVCALNDETIVAAPKPYVEFKGTKILLNDLTKEQLEKVVDYDFGKIDGSFDEV
TLKQYLSSRVVPLRSLLEVIPGSAQLVIRVYFPTDKEIDTIPIKISPFININQFIDKLLL
IIFEHERFLRHSGSGSMRQIVFSSCNWEACSILNWKQPNFPVLLQMKNLLRDSTTGKFVG
DTPNCLKELAVNPQKMSYLNTELINIHTMVQFAMNNNLLGVTLPYEVLKICPSLARIIKQ
NGLLLIASVGENDQIPADGGYSGIYYACELLFENNIDM*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 3537
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable