UBP15 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH202878.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
ScCD00013172
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3693nts
Open reading frame: 1 to 3693

ATGAGCTCTGAAGACGAATTGGGAAGTATCGGTACTGTGTTTCCCGGAAGTCCCATAGAT
AAGAGCATTGGGAGTATTCTCCCACAATTTGATGAAGAAGTGGAGACTTTGCTGGAAGAT
AGCTTCACGTGGAACATTCCTGACTGGAACGAGTTAACAAACCCGAAATACAATTCGCCC
AGGTTTAGAATTGGTGATTTCGAATGGGACATTCTATTATTCCCTCAGGGAAACCATAAT
AAAGGTGTTGCGGTATATCTGGAACCTCATCCGGAAGAAAAATTAGATGAGACTACGGGA
GAGATGGTGCCAGTTGATCCGGACTGGTATTGTTGTGCTCAGTTTGCCATTGGTATATCT
AGACCTGGTAATGGTGACACCATCAATTTAATTAACAAATCGCATCACCGATTCAACGCT
CTAGATACAGACTGGGGATTTGCAAATTTGATAGATTTGAACAACTTGAAACATCCCTCA
AAAGGAAGACCGCTTTCGTTCTTAAACGAAGGGACCTTGAACATAACAGCGTATGTGCGC
ATATTGAAGGATCCTACTGGTGTCTTGTGGCATAATTTCCTGAATTATGACTCGAAGAAA
GTCACGGGCTATGTTGGCTTCCGAAATCAGGGTGCCACATGCTATTTGAATTCGTTATTG
CAATCTTACTTCTTTACAAAATACTTCAGAAAACTGGTTTACGAAATACCCACCGAACAT
GAAAGTCCAAACAACAGTGTCCCATTGGCTTTGCAAAGAGCTTTCTACCAACTACAAGTA
TCCGATATACCATTAGACACTTTGGAACTTACCAGGTCATTCGGTTGGGACACTGCGGAA
TCCTTCACTCAACACGATGTGCAGGAATTGAATAGGATATTAATGGACAGGCTGGAAAAT
AATATGAAGGGAACACCCGTGGAGGGGAAGTTAAATGAAATATTTGTCGGAAAAATGAAA
AGTTATATTAAATGTATCAATGTTGATTATGAATCTGCTAGAGTGGAGGACTTTTGGGAT
CTGCAACTGAATGTCAAAAATTTCAAGAATTTACAAGAATCTTTTGACAATTACATTGAG
ATGGAACTAATGAATGGTGAAAATCAGTATGCTGCTCAAGACTATGGTTTGCAAGATGCT
CAAAAAGGTGTTATATTCGAATCATTTCCGCCAGTTTTACATCTGCAGTTGAAAAGATTT
GAATACGATTTTAATTATGATCAGATGGTAAAGGTTAACGATAAATATGAATTTCCTGAA
ACAATTGACTTGTCGCCCTTCGTTGATAAAGACGTATTAAAGAAAACGTTAGACTCGGAG
AACAAGGATAAGAATCCTTATGTTTATAACTTACATGGTGTGCTAGTTCATTCTGGTGAT
ATATCTACCGGCCACTACTATACTCTGATTAAACCTGGTGTTGAAGATCAATGGTACAGA
TTTGATGATGAAAGGGTTTGGAGGGTCACAAAGAAACAAGTTTTCCAAGAAAATTTTGGA
TGTGATAGGTTGCCTGATGAAAAAGTTCGTACAATGACGAGAGGTGAGTATCAGAACTAT
ATCATTCAACGCCATACAAGTGCTTATATGCTTGTTTATATACGTCAAGAACAAGAGGAG
GATTTGCTGCGTCCAGTTTTGGAATCTGATGTTCCTAAGCATGTAATCACAAGAGTAAGA
GAAGAAATCAAAGAACGGGAAACAAAAGAAAAGGAAATTAGAGAAGCACACCTGTATGTC
ACGCTCAGATTGCACTCAATCAAAGAGTTTATTCATTATGAAGGATTTGACTATTTCGCA
CATGATGGGTTTAGGTTATTTGCAGAGGAGCTCAATGACTCTGGTTTGCAGCAAATCAAC
TTAAAAGTCTTGAGAACAACTAAGTTATCGGATATTTTTGCAAGCATTAAGGAAACTATG
AATATTCCACAAGAAAGGGACGTCAAATATTGGAAAATGGATTATCGCAGAAACAGCACT
TTGCGTTTAACACAACCCATAAATTTTGAATCCGTAAACATCACTTTGCAGGAGGCATTA
AAAAAGGAGAAGAAAAGGACTATGCAAACGCAATATGGAGAGGAAGGTGTTGCTAGTACT
GAGGAAGACGACAAGGCACTTTTAGAAACTGTATCATTCTTAGATCTTTTCATTGAGGAG
CCCTATCTAGAGTTACAATTTTTGAACAAGCTGAAGGAAGCTTCTTTAATCTCAAAGGCC
CAGCTGGATGATGAGCTGATATCAACTATAAGAACGAATCTTCCAGAACTAACAAAGGGA
GGAATCGAGCCTGTTTTTGCCACCGACAATAAATCTAATTTGCTATTCGTCAAGAGCTAC
GACCCACATACCCAAAAGCTATTGGGATTTGGCCATTTTGCCGTAAATCAATTGCAACAG
CTATCGGATATATCTGCAATTATTGAGGACAGCATTTCTTCCAACGAAAAGCTGACTTTC
TACGAAGAAGTCCAACCTGGTACCATAAATGAAATATATATGAAGGAGACAATATATGAT
GCTGATATAGACACGGGTGATATAGTTTCATTTGAGGTTCCAGGTGCTGTTTTGCCAGAT
ACTTTTCCAGTTTACGCAACAATCAAAGATTTTTACTCATATCTGAGGTATCGTGTAAAA
TTGAAGTTTTCTAAATTTGACGGTTCAAGTGAGGAATATGGAGTTAGTAATGAAATCCCA
GAGAGCTTCGAATTCTGGATATCCGCTTATGCACCTTATGATGACCTTGCTAGAATGGTG
TCGAAATATGCGCACGTTAAGCCAGAATATCTAAAGATTATTGCCCTTTATTCTAATGGA
AGATTTGTGTTAAAATCAACCTCACTTTTGAATGATTACCTTTTGAAAGATTTCAATTGC
GATCAAATACCGCCTTTTGCATTTGAAGTATTGTCTGTGCCTTTGAAAGAATTGGAGCGT
TTGAGGCCAATTAAATTATATTGGCTAAAAAATAGTTATATTCATTATCAATGTTTCGAA
TTCGAAGTCGCAAACGATTATACAGAATCTCAATTTCTTGAAAAAGTGCAACACAAGATC
GGCTTTACCGATGAAGAAAAGGAGAACATCTTACTCTGGACCAACACAAATTTCCAGTTT
CAGGGCTTATTATCGGACCAAAACACCTTCAAAGATGTGAGTAAACATTCTTTACTCTTT
GGTAGAATTTTACCCGAGGAGTCTAAATTGTTCAAAGAATTAAATCGTCTAGAAAACGTA
CAAACATCTTCATTGGAGGATTTTATGGATGATGAAAACGCGACTGACAGACCAATGGAC
GATGAGCAAGATCTAGGTATGGCGATAGAACATTCTGAAGATATGAAAGGGCGTATAGTG
GTTGTTCAACAATATTTCAAAGACCTTGAGAATAGACACGGAATATCCTTCTTATTTAAT
TTAATACCAGACGAAACATTCCCAAAGACAAAAGATCGTCTTCATGCAAAATTTGGTCTC
GGACAAAAAGAATTTTCAAAGATCAAATTAAGTATTGGTTACTCCACTGAAGAAGGTACG
GTGTTTAGAAGTTTGCAAGGATTTTCGGACGAAGAATTGGACAAAGTTATATTGTATGAC
ATTATGTCCAACTTAGATTATATCTACATGGATCATCCGGATAGATTGAGATCGCATTCT
TCCTATGATAGACCAATGATCATTAAAAACTAG

Protein sequence predicted based on nucleotide sequence

MSSEDELGSIGTVFPGSPIDKSIGSILPQFDEEVETLLEDSFTWNIPDWNELTNPKYNSP
RFRIGDFEWDILLFPQGNHNKGVAVYLEPHPEEKLDETTGEMVPVDPDWYCCAQFAIGIS
RPGNGDTINLINKSHHRFNALDTDWGFANLIDLNNLKHPSKGRPLSFLNEGTLNITAYVR
ILKDPTGVLWHNFLNYDSKKVTGYVGFRNQGATCYLNSLLQSYFFTKYFRKLVYEIPTEH
ESPNNSVPLALQRAFYQLQVSDIPLDTLELTRSFGWDTAESFTQHDVQELNRILMDRLEN
NMKGTPVEGKLNEIFVGKMKSYIKCINVDYESARVEDFWDLQLNVKNFKNLQESFDNYIE
MELMNGENQYAAQDYGLQDAQKGVIFESFPPVLHLQLKRFEYDFNYDQMVKVNDKYEFPE
TIDLSPFVDKDVLKKTLDSENKDKNPYVYNLHGVLVHSGDISTGHYYTLIKPGVEDQWYR
FDDERVWRVTKKQVFQENFGCDRLPDEKVRTMTRGEYQNYIIQRHTSAYMLVYIRQEQEE
DLLRPVLESDVPKHVITRVREEIKERETKEKEIREAHLYVTLRLHSIKEFIHYEGFDYFA
HDGFRLFAEELNDSGLQQINLKVLRTTKLSDIFASIKETMNIPQERDVKYWKMDYRRNST
LRLTQPINFESVNITLQEALKKEKKRTMQTQYGEEGVASTEEDDKALLETVSFLDLFIEE
PYLELQFLNKLKEASLISKAQLDDELISTIRTNLPELTKGGIEPVFATDNKSNLLFVKSY
DPHTQKLLGFGHFAVNQLQQLSDISAIIEDSISSNEKLTFYEEVQPGTINEIYMKETIYD
ADIDTGDIVSFEVPGAVLPDTFPVYATIKDFYSYLRYRVKLKFSKFDGSSEEYGVSNEIP
ESFEFWISAYAPYDDLARMVSKYAHVKPEYLKIIALYSNGRFVLKSTSLLNDYLLKDFNC
DQIPPFAFEVLSVPLKELERLRPIKLYWLKNSYIHYQCFEFEVANDYTESQFLEKVQHKI
GFTDEEKENILLWTNTNFQFQGLLSDQNTFKDVSKHSLLFGRILPEESKLFKELNRLENV
QTSSLEDFMDDENATDRPMDDEQDLGMAIEHSEDMKGRIVVVQQYFKDLENRHGISFLFN
LIPDETFPKTKDRLHAKFGLGQKEFSKIKLSIGYSTEEGTVFRSLQGFSDEELDKVILYD
IMSNLDYIYMDHPDRLRSHSSYDRPMIIKN*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 3693
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable