ALA1 (S. cerevisiae ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH202967.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
ScCD00013235
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2877nts
Open reading frame: 1 to 2877

ATGACGATCGGTGATAAGCAAAAATGGACCGCTACTAATGTCCGTAATACCTTTCTAGAC
TATTTCAAATCTAAAGAACACAAGTTTGTCAAATCCTCTCCAGTAGTTCCATTTGATGAT
CCAACTTTACTTTTCGCTAATGCCGGTATGAACCAATACAAGCCTATCTTTTTAGGTACT
GTCGATCCAGCTTCCGATTTCTACACCTTGAAAAGGGCTTACAACTCTCAAAAGTGTATC
AGAGCTGGTGGTAAACACAACGATTTAGAAGATGTCGGTAAGGATTCTTATCATCATACC
TTTTTTGAAATGCTGGGTAACTGGTCGTTTGGCGACTATTTCAAGAAGGAAGCTATTACT
TACTCATGGACTTTGTTGACTGAAGTATACGGCATTCCAAAGGATAGATTATACGTTACC
TATTTTGAAGGTGATGAAAAGTTAGGCTTGGAACCTGATACTGAAGCCCGTGAACTATGG
AAAAATGTTGGTGTTCCCGATGACCATATATTACCTGGTAATGCAAAGGACAACTTTTGG
GAAATGGGTGACCAAGGCCCATGTGGTCCTTGTTCTGAAATTCACTACGATAGAATAGGT
GGTAGAAATGCTGCTTCCTTGGTTAATATGGACGACCCTGATGTCTTGGAAGTTTGGAAT
TTGGTTTTCATTCAATTCAACAGAGAACAAGACGGGTCTTTGAAGCCTTTACCTGCCAAG
CATATCGACACAGGTATGGGGTTCGAAAGATTGGTTTCTGTTCTGCAAGATGTTAGATCT
AACTATGATACCGATGTTTTCACACCTTTGTTCGAGCGTATCCAAGAAATCACTTCAGTT
AGACCATATTCTGGCAATTTTGGTGAGAATGACAAGGACGGTATCGATACTGCCTACAGA
GTTTTAGCTGATCATGTCCGTACATTGACTTTTGCCTTAGCTGACGGTGGCGTTCCAAAC
AATGAAGGTAGAGGATATGTTTTGAGACGCATTCTAAGAAGAGGTGCCCGTTACGCCCGT
AAATACATGAATTACCCAATCGGTAACTTTTTCTCCACTTTGGCTCCAACTTTAATTTCT
CAGGTTCAAGATATCTTTCCCGAATTAGCCAAGGATCCTGCATTCCTCTTTGAAATCTTG
GATGAAGAAGAAGCTTCTTTCGCTAAGACCTTGGATCGTGGTGAAAGACTATTTGAAAAA
TATGCTTCTGCTGCTTCTAAGACTGAATCTAAGACTTTAGACGGTAAACAAGTTTGGAGA
CTTTACGACACTTATGGTTTCCCAGTCGACTTGACTGAATTGATGGCTGAAGAACAAGGC
TTGAAGATTGATGGACCCGGTTTCGAAAAGGCCAAACAAGAGTCATACGAAGCATCCAAA
AGAGGTGGTAAGAAGGACCAAAGTGATTTGATTAAGTTAAACGTCCATGAATTATCCGAG
TTGAACGATGCTAAGGTGCCCAAGACTAATGATGAATTCAAATACGGCAGCGCCAACGTC
GAAGGTACCATTTTGAAACTGCATGACGGTACCAACTTTGTTGATGAGATCACTGAACCA
GGTAAGAAGTACGGTATTATTTTGGATAAAACATGTTTCTACGCCGAACAAGGTGGTCAA
GAATATGACACTGGTAAAATTGTTATTGATGACGCTGCTGAGTTTAATGTTGAAAATGTT
CAATTGTATAACGGTTTTGTTTTCCACACCGGGTCTTTAGAAGAAGGTAAGTTGTCTGTC
GGTGACAAGATTATCGCTTCATTCGATGAACTACGTCGTTTCCCTATTAAGAACAATCAT
ACTGGTACACATATCTTAAACTTTGCTCTGAAGGAAACTTTGGGTAATGATGTCGATCAA
AAGGGTTCTTTGGTTGCCCCAGAAAAATTGAGATTCGATTTCTCTCATAAAAAGGCTGTG
TCAAATGAAGAATTGAAAAAAGTTGAAGATATCTGTAATGAGCAAATTAAAGAAAACTTA
CAAGTGTTTTACAAGGAAATTCCATTGGACTTGGCCAAATCCATTGATGGTGTTCGTGCT
GTCTTTGGTGAGACTTACCCAGATCCAGTTCGTGTAGTTTCTGTTGGTAAGCCAATCGAA
GAATTGTTGGCCAACCCAGCTAACGAAGAGTGGACCAAGTATTCTATTGAATTTTGCGGT
GGTACCCATGTCAACAAGACAGGCGATATTAAATACTTCGTCATTTTAGAAGAGAGCGGT
ATTGCAAAGGGTATCAGAAGAATTGTTGCTGTTACTGGAACTGAAGCCTTTGAAGCTCAA
AGATTGGCTGAACAGTTTGCTGCTGATTTGGATGCTGCAGACAAGCTGCCGTTCTCTCCA
ATCAAAGAAAAGAAGTTGAAGGAACTTGGTGTCAAACTTGGTCAACTTTCAATTTCTGTT
ATCACAAAAAATGAACTGAAGCAGAAATTTAACAAAATTGAAAAAGCAGTGAAGGATGAA
GTTAAGTCAAGGGCCAAAAAAGAAAACAAACAAACTCTTGATGAAGTGAAGACATTTTTC
GAAACCAATGAGAATGCTCCATACCTAGTTAAATTCATTGATATTTCTCCAAATGCTAAA
GCTATCACTGAAGCGATCAACTACATGAAATCCAATGATTCTGTCAAAGACAAGTCAATC
TATTTATTGGCAGGTAATGACCCTGAAGGTCGTGTTGCTCACGGTTGTTACATCTCCAAT
GCTGCTTTAGCCAAGGGTATTGATGGTTCTGCGCTTGCCAAAAAGGTGTCCAGTATCATC
GGCGGTAAGGCTGGTGGTAAAGGTAATGTTTTCCAAGGTATGGGTGATAAACCAGCCGCT
ATAAAGGATGCAGTTGATGATTTGGAAAGTTTGTTCAAGGAGAAGCTTTCCATTTAG

Protein sequence predicted based on nucleotide sequence

MTIGDKQKWTATNVRNTFLDYFKSKEHKFVKSSPVVPFDDPTLLFANAGMNQYKPIFLGT
VDPASDFYTLKRAYNSQKCIRAGGKHNDLEDVGKDSYHHTFFEMLGNWSFGDYFKKEAIT
YSWTLLTEVYGIPKDRLYVTYFEGDEKLGLEPDTEARELWKNVGVPDDHILPGNAKDNFW
EMGDQGPCGPCSEIHYDRIGGRNAASLVNMDDPDVLEVWNLVFIQFNREQDGSLKPLPAK
HIDTGMGFERLVSVLQDVRSNYDTDVFTPLFERIQEITSVRPYSGNFGENDKDGIDTAYR
VLADHVRTLTFALADGGVPNNEGRGYVLRRILRRGARYARKYMNYPIGNFFSTLAPTLIS
QVQDIFPELAKDPAFLFEILDEEEASFAKTLDRGERLFEKYASAASKTESKTLDGKQVWR
LYDTYGFPVDLTELMAEEQGLKIDGPGFEKAKQESYEASKRGGKKDQSDLIKLNVHELSE
LNDAKVPKTNDEFKYGSANVEGTILKLHDGTNFVDEITEPGKKYGIILDKTCFYAEQGGQ
EYDTGKIVIDDAAEFNVENVQLYNGFVFHTGSLEEGKLSVGDKIIASFDELRRFPIKNNH
TGTHILNFALKETLGNDVDQKGSLVAPEKLRFDFSHKKAVSNEELKKVEDICNEQIKENL
QVFYKEIPLDLAKSIDGVRAVFGETYPDPVRVVSVGKPIEELLANPANEEWTKYSIEFCG
GTHVNKTGDIKYFVILEESGIAKGIRRIVAVTGTEAFEAQRLAEQFAADLDAADKLPFSP
IKEKKLKELGVKLGQLSISVITKNELKQKFNKIEKAVKDEVKSRAKKENKQTLDEVKTFF
ETNENAPYLVKFIDISPNAKAITEAINYMKSNDSVKDKSIYLLAGNDPEGRVAHGCYISN
AALAKGIDGSALAKKVSSIIGGKAGGKGNVFQGMGDKPAAIKDAVDDLESLFKEKLSI*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 2877
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable