Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
FLH202973.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
ScCD00013239
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3795nts
Open reading frame: 1 to 3795
ATGGTGTTATATAAAAGGAAACCTATATTACTTCCTGATCCAAAACCTTTACCATTGGAT
TTGAATGTACAAGTATGGCATATTGAAGAGACTGGAGAATGGTTTTCTAGTTACGAAGAA
TTTTTAGAAAGGTTTGATTTCTACACACGCCACCATTTTACATGCGAAATCACTGGTACT
TCTTGCCTGACTTTTTTCCAAGCTCTGGATAGTGAGGAGACACAATTCAAATACGTCGAA
GACAGATTCCCATTAAAGTTGAGAGAACCAGTTGCAAGATTTTTACATTTTAACGGAATA
AGAAGGTTAGATGCATTGGTGGAGAAAGTTTACGCAAGATTTAAAAATGACTTTTTCCCA
GGAGAAGTTGTTTATCTACGTAAACAGAAAGACTCTTCTACCACAAGCTCTAATTCCCAA
CAAAGTACTCCTCAACCTGATGATATGGTCGAAATCAACAGTGTTGGTAATCCTGGCTTA
CCGCAGTACCAATACCAAAGACGTTATGTTATCAAAGAAAAGGTCCAATTTAACGCAACG
ATAAATCCAGAGAGCAGGGAAATTGTGATGCCCGCTCATACTAAATACATGCTGATAGAA
GAAGCAGCTTCCAGTAATAAATCCTTCATTGTTGATCAAGGACAAATTTACAGGGACCGT
TCCACTTTCACTAAGCACTTAATCAAATGTTTTTTCAAGATTACTTTACAGAGGGCATCT
TCGAAAATGGGTGCACCTTGGTGTGTCAAACCGGAATATTTAGCGATGTATGGTTTAACG
ATGGAATGGCCAAAAGATATGTTAAAATATAAAGAAGATGAACCTGTCGTAGCAAGACGT
TCCAACAGTGCCAATGTTTCATCCCCAGAAAGTGAAAAGAATAAACGTCAAAGTAAATCA
TCGGGTAAAAGTAACACCTCAAACGATGCTTCTAACAAAAAAGAAACCAAGAAAAAAAGA
AAGCCAACCGAAGTAAATGATTCTGAGAATAATTCTTCGGAAGAAGACAAGAAGAAGGGT
CAAAATGTCACATCAGAAACACATTCTAAAAAAAGGAAAAAGGAAGCCAATGAGGAACCG
AACACGGAAAACGTCGAAAGCGTTCCTACACCAGCTAATGCGGAGCCTCAGGCTGTAACA
ATAACGAGCATAATGGATGACCTAGCTTTGCCATATCAACATCCACCGAACATCTTTCCA
AACTTAACGTATTATAATGAAAAATTAGAATGTATTTCATTAGGTTCTACAAAACTTTCC
AGACCTTTTGATTCTTTTGGAAAGCTATTACAAGCCTATCAGTTTCTGAATACGTTTGGT
TCGAAAATTTGTCTCTCTCATTTTAGCCTTGACCAATTCATTACTTCTTTGAAATGTACA
GACCCATATGAGTTAAAGGGCGAAGTTGTTTTAGTGAACATAAGAACGCAAACGTCTAAA
GAACAAGAAATTGAAAATAACGGCCTGCCGATGAAAAACAAAGCAGAGACTACTACTGAG
GAAGACTCTGAAAACCCAAGTGACTGGCAAAGAAATTCATTTATTCGTGATATGATAATG
AAAAGAAATTCCGACAAGGTTGAGTACAAGATTGTTCACGATGACCCAGCATCCGATGAT
ATACTTGATAATATCAATCATAATGGGTCTGCTCTTCTCATTGAAGTTTTTACTGCGCTC
TTACGTTTGTTCATCAACGAAGAAGGCGATTGGAGTTGCATCGTCGTTGAAAACTGGATA
ATAGACGACAAGGGAGTCCTTATGGAGAGAAAAGATGAAAGAGGGGAGGGGGAGGCGAAG
CAAAAACGAAATGCTCACGGATACTTTTTACAAGATAAGGAAAAGATCGACAACCTAAAG
GACACATTAAAGGAAAACGCAACAGAAGTTCAAAAAGAATCAGACGCTAAAAACGAAACA
AATTCAGAATCTGATTCTAAATCTGATTCTGACAGTGAAGAACGAGATCCTAAATTAGAA
AAGTGTTTGAACTACCGCAATGTTAACTGGATAGAACGCTTGACGAAAAGGCAATTTAAT
AACAGTTACTGGCTAATAATTTTACTGGGAGTCTTAGAAGATTGTAGACATCTTCCCATG
TATACAGAGTTTATTGATTCTTTCATAGAAAAGATCATACCCAAAGATATATCAGCTACT
CAATTGCCCAAACAGCTTTGGAGAAATTTCTGTCGAAAGCTGTCGTTCAGTGACAAAGTC
AATGCGCTGTGGATACTTGTAGATCTCGTATCGCATTTTTCTCCAGATATCAAAGCTGCT
GTAGATGATTCCATGGAACTATGTGGTCAAATTCGTAGCGAAAGATTCAAAGTTGCGAGA
GAGTTGAAAACGGAGGCAGCCGTCTTAAGTAATCTTCAAGGCGACCTGCAGGCAATTCAA
GAGAAGTTAAATAAAACAGATGAAAACACACCATCAGCCGATGGTGCCGATAAAAAAGAT
GATTCCGAATCAAATAGTGAACCAATTGATCTTATTATCATTGAGAAAAAGCAAAAGTTA
ATTGAAGAGCAGGATAAAAAGGTACAAGCTTTACAATCAGATAAAAATTTCCTCGATAAT
TGTCTATTTGAAAACGATCTACAAAGATTAAAGCCGTTGGGATTGGATCGATACGGGAAC
AGGTATTTTTGGCTGGATCACAATGGTGTTCCATTTCCTCAATATCCTGCTGGTATGAAT
GAGACGCCGAAAAGTAACAATAGTTTGAGTTATCACTCCGGTCGTTTATTAATTCAGGGA
CCTAAAGCATCATCGGCGAAATTCTTTTTGAACGTCAGTGATGAGCAATTGAGCAATTGG
CAAAAAATCAGAAATTCCGAAGGAATATCCGAGGCAACTAGGGAAGTATTTGGTATCTCC
AAAACTAGTTCCGGATCTTATAATTACGTAGAAAATGGAATAGAGGTCGAACTATTGGAC
AGTAATGATCGCGTAAACCCACTTATAGAGCTGACACCTATTCAAAAGAAAATTATGGAT
GAAACACCTTCAAGGTTGCTACTATCACCAGATCAATGGTATTGCATTGACAAGTTAGAG
GACTTGTCAAGAATAATGGACTGGTTAGATAATTGGGGTCGCAAAGAGCATGATCTCTTG
AGGCAAATCAGACCCATAATGGAACGAATTAAAAGTTCCCTTAGCTTGCGCGACCATGCG
CTGTCACTGACAGCATTCACCAAAAATGAAGAAAAGTTGCTAAAGGAGTTAGAAAATAAT
GAATTTACCGAAAACGAATTGAACGTTGATAGTATGGATGTTGATGATAAAAATAGCGGT
GTTAAAAGTGAGGTTGATGTACAGGTTGATGCCGAAGAGAAGAGAGAAGCTGTGATAGAC
GAAAAGTTGGAGGTGATTGCAGACGAACTCATGAAGCTAGATGATAGCTCGAAAACACGC
AATGTTCTTAATAGAATACAAGAATTAGAAGATCAGAGAGATGAACTCTTGGAACAGAAA
AAATCCATAATAAATTCGCAGAGGCCAGGCGCGAGGATTTTAGCCCGTTCAGAAAGGAAA
AGAACCAAAATCTCCCGTGGTAATAAGGTGAATAAGCAGATTGAAATATTGACTGACTTA
GTCAATTATAGACATTTTAAAGCTATGGAAGATGTGATAGCTTGGAAAAACGTTTTAGCA
AATTCGATATGGGGTTCCTCACTTCGCAAAAATGCATCTGGTAATAAAAGGAGTGGTGTC
ATTGAAACAGTAGACGATAAATTAAAAGATATAGTGGGCCAAACCTCAAGAACAGTAACA
CCTGCCCCAAATTAG
Protein sequence predicted based on nucleotide sequence
MVLYKRKPILLPDPKPLPLDLNVQVWHIEETGEWFSSYEEFLERFDFYTRHHFTCEITGT SCLTFFQALDSEETQFKYVEDRFPLKLREPVARFLHFNGIRRLDALVEKVYARFKNDFFP GEVVYLRKQKDSSTTSSNSQQSTPQPDDMVEINSVGNPGLPQYQYQRRYVIKEKVQFNAT INPESREIVMPAHTKYMLIEEAASSNKSFIVDQGQIYRDRSTFTKHLIKCFFKITLQRAS SKMGAPWCVKPEYLAMYGLTMEWPKDMLKYKEDEPVVARRSNSANVSSPESEKNKRQSKS SGKSNTSNDASNKKETKKKRKPTEVNDSENNSSEEDKKKGQNVTSETHSKKRKKEANEEP NTENVESVPTPANAEPQAVTITSIMDDLALPYQHPPNIFPNLTYYNEKLECISLGSTKLS RPFDSFGKLLQAYQFLNTFGSKICLSHFSLDQFITSLKCTDPYELKGEVVLVNIRTQTSK EQEIENNGLPMKNKAETTTEEDSENPSDWQRNSFIRDMIMKRNSDKVEYKIVHDDPASDD ILDNINHNGSALLIEVFTALLRLFINEEGDWSCIVVENWIIDDKGVLMERKDERGEGEAK QKRNAHGYFLQDKEKIDNLKDTLKENATEVQKESDAKNETNSESDSKSDSDSEERDPKLE KCLNYRNVNWIERLTKRQFNNSYWLIILLGVLEDCRHLPMYTEFIDSFIEKIIPKDISAT QLPKQLWRNFCRKLSFSDKVNALWILVDLVSHFSPDIKAAVDDSMELCGQIRSERFKVAR ELKTEAAVLSNLQGDLQAIQEKLNKTDENTPSADGADKKDDSESNSEPIDLIIIEKKQKL IEEQDKKVQALQSDKNFLDNCLFENDLQRLKPLGLDRYGNRYFWLDHNGVPFPQYPAGMN ETPKSNNSLSYHSGRLLIQGPKASSAKFFLNVSDEQLSNWQKIRNSEGISEATREVFGIS KTSSGSYNYVENGIEVELLDSNDRVNPLIELTPIQKKIMDETPSRLLLSPDQWYCIDKLE DLSRIMDWLDNWGRKEHDLLRQIRPIMERIKSSLSLRDHALSLTAFTKNEEKLLKELENN EFTENELNVDSMDVDDKNSGVKSEVDVQVDAEEKREAVIDEKLEVIADELMKLDDSSKTR NVLNRIQELEDQRDELLEQKKSIINSQRPGARILARSERKRTKISRGNKVNKQIEILTDL VNYRHFKAMEDVIAWKNVLANSIWGSSLRKNASGNKRSGVIETVDDKLKDIVGQTSRTVT PAPN*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
3795
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
